Monoclonal anti IgA Immunoglobulin (IA761)

BNUB0761-0
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Product Description

This MAb is specific to heavy chain of IgA and shows minimal cross-reaction with heavy chains of other immunoglobulins. It is reactive with all subclasses of Alpha heavy chain. Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through inter-chain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin A (IgA) is the main protein of the mucosal immune system. It is generated by B-cells in gut-associated lymphoid tissues. Daily production of IgA exceeds that of any of the other immunoglobulins.IgA exists mainly in dimers but can also exist as polymers or as monomers. Dimers and polymers contain a joining (J) chain that can be bound by the polymeric immunoglobulin receptor (pIgR) for transportation of the molecule to mucosal surfaces. The most common feature of plasmacytomas, and certain non-Hodgkin s lymphomas is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Additional Information

Conjugation

Purified with BSA, 0.2 mg/mL, Purified, BSA-free, 1 mg/mL, CF405S, CF405M, CF488A, CF543, CF555, CF568, CF594, CF640R, CF647, CF660R, CF680, CF680R, CF770, Biotin, R-PE, APC, Per-CP, Alkaline Phosphatase, HRP

Size

50 uL, 100 uL, 250 uL, 500 uL

Supplied As

Purified: 0.2 mg/mL in PBS/0.05% BSA/0.05% azide, Purified, BSA-free: 1 mg/mL in PBS without azide, Conjugates: 0.1 mg/mL in PBS/0.1% BSA/0.05% azide, HRP conjugates: 0.1 mg/mL in PBS/0.05% BSA

Antibody type

Primary

Host species

Mouse

Reactivity (target)

IgA Immunoglobulin (IA761)

Clonality

Monoclonal

Isotype

IgG1

Clone

IA761

Molecular weight

50-75 kDa

Synonyms

A2m Marker; Ig alpha 1 Chain C Region; Ig alpha 2 Chain C Region; IGHA1; IGHA2; Immunoglobulin Am1; Immunoglobulin Am2; Immunoglobulin Heavy Constant Alpha 1; Immunoglobulin Heavy Constant Alpha 2

Entrez gene ID

3493 (IGHA1), 3494 (IGHA2)

SwissProt

P01876 (IGHA1), P01877 (IGHA2)

Unigene

699841

Immunogen

Purified human alpha heavy chain

Species reactivity

Human

Applications

Flow cytometry, Immunofluorescence, Immunohistology (formalin)

Cellular localization

Cytoplasm, Cell Surface and Secreted

Application notes

Flow Cytometry 1-2 ug/million cells in 0.1 mL, Immunofluorescence 0.5-1.0 ug/mL 30 min RT, Immunohistology formalin-fixed 0.5-1.0 ug/mL 30 min RT, Optimal dilution for a specific application should be determined by user, Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Positive control

Daudi, 293T, Raji or hPBL cells. Tonsil or Spleen.

MSDS (PDF):MSDS Primary antibodies

Protocol (PDF): Protocols for antibody-based detection

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