Support & Resources

+1

NucSpot® 470 Nuclear Stain, 1000X in DMSO

NucSpot® 470 is cell membrane-impermeant, nuclear-specific green fluorescent DNA stain for the FITC channel.

Product Attributes

Cellular localization

Nucleus

For live or fixed cells

For fixed cells

Assay type/options

No-wash staining

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Fixation options

Permeabilize before staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Green

Storage Conditions

Store at -10 to -35 °C

Excitation/Emission

460/546 nm (with DNA)

NucSpot® 470 Features:

  • Nuclear-specific green counterstain for fixed cells
  • Selectively stains dead cells in live culture
  • No wash required
  • Abs/Em 460/546 nm for FITC channel
  • Excellent match for blue LED excitation sources
Size
Catalog #
price
Qty
20 uL
200 uL
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Protocols
SDS

Product Description

NucSpot® 470 is a cell membrane-impermeant green fluorescent DNA stain. It is virtually non-fluorescent in the absence of DNA, but fluoresces bright green upon DNA binding. While other green nucleic acid stains like TOTO®, TO-PRO®, or SYTOX® dyes stain both the nucleus and cytoplasm, NucSpot® 470 specifically stains the nucleus of fixed and permeabilized cells. It also can be used to selectively stain dead cells in living cultures.

NucSpot® 470 is nuclear-specific in dead or permeabilized yeast cells. It is dead cell specific in gram-negative bacteria. In gram-positive strains there may be live cell staining. See our Cellular Stains Table for more information on how our dyes stain various organisms.

NucSpot® 470 has green fluorescence (Ex/Em 460/546 nm) that can be imaged using standard settings for FITC. With excitation at 460 nm, it also is an excellent match for instruments with blue LED excitation sources.

Learn more about our full selection of Cellular Stains for the nucleus and other organelles.

TOTO, TO-PRO, and SYTOX are registered trademarks of Thermo Fisher Scientific.

 

 

You may also like…