Support & Resources

+1

PMA Real-Time PCR Bacterial Viability Kit – E. coli O157:H7 (Z3276)

A kit designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the Z3276 gene.

Product Attributes

Viability dye

PMA, PMAxx™

Storage Conditions

Store at -10 to -35 °C

Viability dye
Size
Catalog #
price
Qty
200 assays
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

This kit is designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMA or PMAxx™) and primers to amplify the Z3276 gene.

Features

  • Selectively detect viable E. coli O157:H7 using qPCR
  • Contains primers to amplify the Z3276 gene
  • Works even in complex samples or mixed cultures
  • Sufficient reagents to treat 80 bacterial cultures with PMA or PMAxx™
  • Sufficient reagents for 200 PCR reactions

Kit Components

  • PMA dye or PMAxx™ dye
  • PMA Enhancer for Gram Negative Bacteria
  • Forget-Me-Not™ EvaGreen® qPCR Master Mix
  • ROX reference dye
  • Z3276 primer mix, 5 uM

About this kit

E. coli O157:H7 is a pathogenic strain of Escherichia coli bacteria. Often a food-borne pathogen, it can cause digestive illness. PMA-based viability PCR to detect E. coli O157:H7 has been reported using the primers provided in the kit, and ORF Z3276 was shown to be specific for strain O157:H7 . These primers have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix. For pan-E. coli primers to detect other strains of E. coli besides O157:H7, please see kit 31050.

To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.

Strain-Specific Bacterial Viability PCR Kits

Bacteria StrainGene nameKit with PMA (catalog #)Kit with PMAxx™ (catalog #)
Salmonella entericainvA3103331033-X
Mycobacterium tuberculosisgroEL231034N/A
Staphylococcus aureusnuc31035N/A
MRSAmecA31036N/A
E. coli O157:H7Z32763103731037-X
E. coliuidA3105031050-X
Listeria monocytogeneshly3105131051-X
Legionella pneumophilamip31053N/A

Don’t see your favorite strain? Let us know at techsupport@biotium.com.

PMAxx technology is covered by granted and/or pending US and international patents.

References

Find a list of PMA and PMAxx™ references and a list of validated bacterial strains under Supporting Documents.

 

You may also like…