PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)
A kit designed to perform viability PCR on Listeria monocytogenes. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the hly gene.
Wishlist updated! View wishlist
Product Description
This kit is designed to perform viability PCR on Listeria monocytogenes. It contains a viability PCR dye (PMA or PMAxx™) and primers to amplify the hly gene.
Features
- Selectively detect viable Listeria monocytogenes using qPCR
- Contains primers to amplify the hly gene
- Works even in complex samples or mixed cultures
- Sufficient reagents to treat 80 bacterial cultures with PMA or PMAxx™
- Sufficient reagents for 200 PCR reactions
Kit Components
- PMA dye or PMAxx™ dye
- PMA Enhancer for Gram Negative Bacteria
- Forget-Me-Not™ EvaGreen® qPCR Master Mix
- ROX reference dye
- hly primer mix, 5 uM
About this kit
Listeria monocytogenes is a food-borne pathogen that can cause digestive illness and fetal harm. The gene hly encodes the Listeria Lysin O (LLO) protein that is specific for Listeria. PMA-PCR using these primers for viability PCR in Listeria has been published. In addition, these primers have been validated at Biotium for real-time qPCR using Forget-Me-Not™ EvaGreen® qPCR Master Mix.
To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.
Strain-Specific Bacterial Viability PCR Kits
| Bacteria Strain | Gene name | Kit with PMA (catalog #) | Kit with PMAxx™ (catalog #) |
|---|---|---|---|
| Salmonella enterica | invA | 31033 | 31033-X |
| Mycobacterium tuberculosis | groEL2 | 31034 | N/A |
| Staphylococcus aureus | nuc | 31035 | N/A |
| MRSA | mecA | 31036 | N/A |
| E. coli O157:H7 | Z3276 | 31037 | 31037-X |
| E. coli | uidA | 31050 | 31050-X |
| Listeria monocytogenes | hly | 31051 | 31051-X |
| Legionella pneumophila | mip | 31053 | N/A |
Don’t see your favorite strain? Let us know at techsupport@biotium.com.
References
Download PMA and PMAxx™ References and a list of PMA and PMAxx™ Validated Bacterial Strains.