PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (mecA)
A kit designed to perform viability PCR on MRSA. It contains the viability PCR dye PMA and primers to amplify the mecA gene.
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Product Description
This kit is designed to perform viability PCR on Methicillin-resistant Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the mecA gene.
Features
- Selectively detect viable Methicillin-resistant Staphylococcus aureus using qPCR
- Contains primers to amplify the mecA gene
- Works even in complex samples or mixed cultures
- Sufficient reagents to treat 80 bacterial cultures with PMA
- Sufficient reagents for 200 PCR reactions
Kit components
- PMA dye
- Forget-Me-Not™ EvaGreen® qPCR Master Mix
- ROX reference dye
- mecA primer mix, 5 uM
About this kit
Staphylococcus aureus is an anaerobic gram negative bacterium that can cause of a variety of infections in humans, and methicillin-resistant S. aureus is a rising cause of hospital-acquired infections. The mecA gene confers methicillin-resistance in S. aureus and other methicillin-resistant bacterial species. The mecA primers included in the kit have been used for detection of methicillin-resistant S. aureus, and have been validated for qPCR using Biotium’s Forget-Me-Not™ EvaGreen® qPCR Master Mix. Note that the mecA primers are not specific to S. aureus, because mecA is expressed by other methicillin-resistant bacteria. Biotium also offers the Staphylococcus aureus (nuc) PMA-PCR kit, which includes primers against the S. aureus-specific nuc gene, expressed by methicillin-susceptible and methicillin-resistant S. aureus strains.
To learn more about the advantages of determining bacterial viability using viability PCR, visit the Viability PCR Technology Page.
Strain-Specific Bacterial Viability PCR Kits
| Bacteria Strain | Gene name | Kit with PMA (catalog #) | Kit with PMAxx™ (catalog #) |
|---|---|---|---|
| Salmonella enterica | invA | 31033 | 31033-X |
| Mycobacterium tuberculosis | groEL2 | 31034 | N/A |
| Staphylococcus aureus | nuc | 31035 | N/A |
| MRSA | mecA | 31036 | N/A |
| E. coli O157:H7 | Z3276 | 31037 | 31037-X |
| E. coli | uidA | 31050 | 31050-X |
| Listeria monocytogenes | hly | 31051 | 31051-X |
| Legionella pneumophila | mip | 31053 | N/A |
Don’t see your favorite strain? Let us know at techsupport@biotium.com.
References
Download PMA and PMAxx™ References and a list of PMA and PMAxx™ Validated Bacterial Strains.