Cytosolic Tracers
Biotium offers an array of fluorescent cytosolic tracers for various cell types and applications:
ViaFluor® SE Cell Proliferation Dyes are non-toxic and can be used to stain cells for long-term, fixable cell tracing for flow or microscopy. CFDA and Calcein AM are membrane-permeant esterase substrates with green fluorescence. CF® Dye-labeled molecules such as Dextran and BSA can be used to observe endocytosis. Polar tracers used to trace neurons and cell junctions include CF® Dye-labeled Biocytin, Hydrazide, and Fluoro-Gold™.
See our full selection of cytosolic tracers on our Cellular Stains page.
ViaFluor® SE Cell Proliferation Kits
30068-T, 30068, 30086-T, 30086, - 30050View allHide
Fixable cytoplasmic stains for monitoring cell division by flow cytometry. The dyes can also be used to track cell populations in co-culture.
CF® Dye Hydrazide
96063, 96064, 92136, 92151, 92152, 92153, 92154, 92156, 92157, 92158, 92183, 96024, - 96025View allHide
Fluorescent CF® dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.
Bovine Serum Albumin CF® Dye Conjugates
20289, 20290, 20291, - 20292View allHide
Bright and photostable fluorescent BSA conjugates for applications in which well-defined molecular weight fluid phase tracers are needed. These applications include studying the rate of endocytosis and exocytosis and following vesicle trafficking.
CF® Dye Dextran 10,000 MW, Anionic and Fixable
80110, 80111, 80112, 80113, 80114, 80115, 80116, 80118, 80119, 80120, - 80121View allHide
CF® Dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10,000 MW, and contains a formaldehyde-fixable free-amine group.
CF® Dye Dextran 150,000 MW, Anionic and Fixable
80124, 80131, 80132, - 80133View allHide
CF® Dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group; available with a selection of CF® Dyes.
CF® Dye Dextran 250,000 MW, Anionic and Fixable
80125, 80134, 80135, - 80136View allHide
This dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group; available with a selection of CF® dyes.
CF® Dye Dextran 3,500 MW, Anionic and Fixable
CF® Dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 3,500 MW, and contains a formaldehyde-fixable free-amine group.
CF® Dye Dextran 40,000 MW, Anionic and Fixable
80122, 80126, 80127, - 80128View allHide
This dextran is 40,000 MW, and contains a formaldehyde-fixable free-amine group; available with a selection of CF® dyes.
CF® Dye Dextran 70,000 MW, Anionic and Fixable
80138, 80117, 80141, 80129, 80130, - 80123View allHide
This dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group; available with a selection of CF® Dyes.
CF®DI Hydrazide
92164, 92165, 92166, 92184, - 92185View allHide
CF®DI dye hydrazides react with carbonyl moieties of aldehyde or ketone groups. They are size- and charge-matched for detection of protein carbonylation by fluorescence two-dimensional differential gel electrophoresis (2D-DIGE).
5-(and 6)-CFDA
5(6)-CFDA (full name: 5-(and-6)-Carboxyfluorescein diacetate) is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein (see #51013 ).
5-(and-6)-Carboxy-2′,7′- dichlorofluorescein
5-(and-6)-carboxy-2′,7′-dichlorofluorescein is similar to carboxyfluorescein (51013), but has lower pKa. It is useful for detecting pH in more acidic environment.
5-(and-6)-Carboxyfluorescein
5-(and-6)-FAM (full name: 5-(and-6)-Carboxyfluorescein mixed isomer) has a pKa of 6.5 and can be used as a pH indicator.
5-Carboxy-2′,7′-dichlorofluorescein (single isomer)
This dye can be used as a low pH indicator similar to 51015, and is also useful as a general green fluorescent label.
5-Carboxy-2′,7′-dichlorosulfonefluorescein
The pKa of 5-carboxy-2′,7′-dichlorosulfonefluorescein is near 4 and thus the dye is potentially an excellent pH indicator for acidic organelles.
5-CFDA (5-Carboxyfluorescein diacetate, single isomer)
5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).