Membrane Potential & Nerve Terminal Dyes
SynaptoGreen™ C2 (Equivalent to FM®2-10)
70044, - 70045View allHide
SynaptoGreen™ C2 is more water soluble than SynaptoGreen™ C4 and thus has a faster de-staining rate than the latter.
AM4-65
AM4-65 is a fixable red fluorescent nerve terminal probe. The dye is spectrally identical to SynaptoRed™ C2 (equivalent to FM®4-64), but it has an additional amine group at the hydrophilic end of the molecule, making the dye fixable with formaldehyde or gluteraldehyde.
SynaptoRed™ C1
70040, - 70041View allHide
SynaptoRed™ C1 is similar to the widely used red fluorescent nerve terminal dye SynaptoRed™ C2 (also called FM®4-64) except that the lipophilic tail of SynaptoRed™ C1 is one carbon shorter than that of the SynaptoRed™ C2.
AM1-44
AM1-44 is a version of AM1-43 (catalog no. 70024) with improved fixability. The dye can also be used as a general probe for following endocytosis.
AM2-10
AM2-10 is a fixable analog of SynaptoGreen™ C2.
SCAS
SCAS (4-Sulfonato calix[8]arene, sodium salt) is a quencher developed by Biotium to reduce background fluorescence when using our fixable AM dyes, SynaptoGreen and SynaptoRed dyes. Unlike ADVASEP-7, SCAS dramatically lowers background fluorescence as soon as it is added to the preparation without the need for repeated wash steps.
SynaptoRed™ C2M (Equivalent to FM®5-95)
70019, - 70028View allHide
SynaptoRed™ C2M is a red fluorescent probe used to follow synaptic vesicles.
ADVASEP-7
ADVASEP-7 is a sulfonated β-cyclodextrin derivative that has been reported to reduce background fluorescence when using SynaptoGreen C4 (also called FM1-43, catalog no. 70020) to stain brain slices.
AM1-43 (Equivalent to FM®1-43FX)
AM1-43 (equivalent to FM®1-43FX) is a fixable, activity-dependent fluorescent nerve terminal probe. AM1-43 functions similarly similarly to SynaptoGreen C4 (catalog # 70020), but with the additional property of being fixable.
AM4-64
AM4-64 is a fixable, activity-dependent fluorescent nerve terminal probe. AM4-64 functions similarly similarly as SynaptoRed™ C2 (#70021) (equivalent to FM®4-64), but with the additional property of being fixable.
SynaptoGreen™ C3
70023, - 70026View allHide
SynaptoGreen™ C3 is similar to SynaptoGreen™ C4 (70020), except that the lipophilic tail is one carbon shorter and also the hydrophilic end is a trimethylammonium group.
SynaptoGreen™ C4 (Equivalent to FM®1-43)
70020, - 70022View allHide
A widely used green fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the red fluorescent dye SynaptoRed™ (70021), synapses or neuromuscular junctions can be imaged independently in two colors.
SynaptoRed™ C2 (Equivalent to FM®4-64)
70021, - 70027View allHide
A widely used red fluorescent dye for following synaptic activity by staining synaptic vesicles at the synapse or neuromuscular junctions. When used in combination with the green fluorescent dye SynaptoGreen™ (70020), synapses or neuromuscular junctions can be imaged independently in two colors. SynaptoRed™ has also been used to study endocytosis in Dictyostelia.
DiIC1(5) (1,1′-dimethyl-3,3,3′,3′-tetramethylindodicarbocyanine, iodide)
The carbocyanine dye DiIC1(5) has been used to measure mitochondrial membrane potential in apoptotic cells. Loss of membrane potential in apoptotic cells is reflected by a decrease of fluorescence signal in the infrared channel.
TMRE
TMRE (Tetramethylrhodamine ethyl ester, perchlorate) and TMRM (70017) are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation.
DiOC16(3)
DiOC16(3) is a green fluorescent lipophilic cyanine dye with two C16 hydrocarbon chains that can be used to stain cytoplasmic membranes.