Cell & Organelle Stains

This dye can be used as a low pH indicator similar to 51015, and is also useful as a general green fluorescent label.

The pKa of 5-carboxy-2′,7′-dichlorosulfonefluorescein is near 4 and thus the dye is potentially an excellent pH indicator for acidic organelles.

5-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 5-CFDA is hydrolyzed by intracellular esterases to 5-carboxyfluorescein (see 50015).

6-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 6-CFDA is hydrolyzed by intracellular esterases to 6-carboxyfluorescein.

7-AAD (7-aminoactinomycin D) is a membrane-impermeant fluorescent DNA binding dye that is useful for live/dead discrimination and cell cycle profiling by flow cytometry.

Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm). The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies.

Actinomycin D is a nonfluorescent GC-selective intercalator. Similar to 7-AAD (cat. no. 40038), actinomycin D has also been used for chromosome banding studies.

ADVASEP-7 is a sulfonated β-cyclodextrin derivative that has been reported to reduce background fluorescence when using SynaptoGreen C4 (also called FM1-43, catalog no. 70020) to stain brain slices.

AM1-43 (equivalent to FM®1-43FX) is a fixable, activity-dependent fluorescent nerve terminal probe. AM1-43 functions similarly similarly to SynaptoGreen C4 (catalog # 70020), but with the additional property of being fixable.

AM1-44 is a version of AM1-43 (catalog no. 70024) with improved fixability. The dye can also be used as a general probe for following endocytosis.

AM2-10 is a fixable analog of SynaptoGreen™ C2.

AM3-25 is a lipophilic styryl dye that has an amine group at the hydrophilic head. The amine group reacts with formaldehyde or glutaldehyde to make the dye fixable.

AM4-64 is a fixable, activity-dependent fluorescent nerve terminal probe. AM4-64 functions similarly similarly as SynaptoRed™ C2 (#70021) (equivalent to FM®4-64), but with the additional property of being fixable.

AM4-65 is a fixable red fluorescent nerve terminal probe. The dye is spectrally identical to SynaptoRed™ C2 (equivalent to FM®4-64), but it has an additional amine group at the hydrophilic end of the molecule, making the dye fixable with formaldehyde or gluteraldehyde.

A fixable red fluorescent nerve terminal probe that is spectrally identical to SynaptoRed™ C2 (also called FM®4-64).

An improved and more soluble formulation of the JC-1 dye used for ratiometric detection of mitochondrial potential changes.