Nucleic Acid Binding Dyes

Thiazole Orange Homodimer (TOhD), also known as TOTO®-1, is a cell-impermeant, high-affinity, fluorogenic green nucleic acid stain.

Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells, to stain DNA in agarose gels and capillary electrophoresis. Thiazole Orange stains the yeast nucleus, and is also able to stain bacteria and mammalian cells.

The amine reactive succinimidyl ester of AO (acridine orange) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugates with green fluoroscence are able to complex with nucleic acids, resulting in green fluorescence nucleic acid conjugate adduct.

The amine reactive succinimidyl ester of MB (methylene blue) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugate will have a blue color and be able to complex with nucleic acids.

The amine reactive succinimidyl ester of TO (thiazole orange) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugates are expected to be essentially nonfluorescent until they are able to complex with nucleic acids, resulting in green fluorescence.

The amine reactive succinimidyl ester of EB (ethidium bromide) can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugates are expected to be essentially nonfluorescent until they are able to complex with nucleic acids, resulting in red fluorescence.

Viability PCR dye, a new and improved version of the popular viability dye propidium monoazide (PMA).

A patented green fluorescent nucleic acid dye with features that are ideal for applications such as qPCR, HRM®, LAMP, and digital PCR. Highly concentrated for customers with specialized applications requiring a concentrated dye solution.

Actinomycin D is a nonfluorescent GC-selective intercalator. Similar to 7-AAD (cat. no. 40038), actinomycin D has also been used for chromosome banding studies.

A far-red cell membrane-impermeant nuclear dye with greater nuclear specificity than Draq7™. Ideal for fixed cell nuclear counterstaining with minimal cytoplasmic RNA staining.

A red fluorescent dead cell stain for bacteria and mammalian cells

A far-red live cell nuclear stain similar to Draq5™.

A red fluorescent dead cell stain for bacteria and mammalian cells that is brighter than Ethidium Homodimer I.

Ethidium bromide (EtBr) is a widely used nucleic acid gel stain although it is also a known potent mutagen. EtBr is an intercalating DNA-binding dye with little sequence preference.

Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm). The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies.

A high purity grade, red fluorescent nucleic acid dye for detecting nucleic acids in solution or for selectively staining dead cells with damaged plasma membranes.