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Acridine Orange, 10 mg/mL in Water (High Purity) (AO)

Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm).The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies.

Product Attributes

Probe cellular localization

Nucleus & cytoplasm

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

No-wash staining

Cell permeability

Membrane permeant

Colors

Green/Red

Excitation/Emission

500/526 nm (DNA), 460/650 nm (RNA)

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Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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10 mL
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Product Description

Acridine orange (AO) stains dsDNA green (525 nm) and RNA or single stranded DNA red (650 nm). The dye is membrane-permeant and its nucleic acid binding property has been used for cell-cycle studies.2,3 Acridine orange has also been used for the detection of microorganisms in cerebrospinal fluid and other clinical specimens.We offer a highly purified form of acridine orange while most of the other commercially available grades of AO are either in zinc chloride complex form or of low purity.

  • λExEm = 500/526 nm (DNA), 460/650 nm (RNA)
  • Yellow solution
  • Store at 4°C and protect from light
  • C17H20ClN3
  • MW: 301.82

References

1. Cytometry 12(4), 330 (1991).

2. Methods Cell Biol 33, 285 (1990).

3. J Cell Physiol 143(2), 279 (1990).

4. J. Clin. Microbiol 14(2), 201 (1981).

 

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