Thiol-Reactive Dyes
Maleimide reacts with thiol groups to form thioether-coupled products. The reaction can take place at pH 7 in the presence of amines. At neutral pH, the maleimide group does not react with histidine or arginine. Maleimide labeling can be a useful alternative to succinimidyl ester labeling in cases where modification of lysines alters antibody binding affinity or other biological activity of the target protein. We offer more than 20 bright and photostable CF® Dye Maleimides.
Methanethiosulfonate (MTS) is a fast-reacting and highly selective thiol-reactive functional group. We offer CF® Dye MTS with a selection of popular colors. We also offer MTS thiol-modifying and cross-linking agents.
We also offer many classic thiol-reactive dyes and biotin, as well as reducing agents commonly used in thiol conjugation protocols.
MTS-BCN
MTS-BCN is a fast-reacting and highly selective thiol-reactive label for attaching BCN to thiol groups on biomolecules. BCN, an alternative to DIBO and DBCO, reacts with azide to form 1,2,3-triazole by copper free 1,3-dipolar Huisgen cycloaddition.
CPMTS
Biotium developed CPMTS as an alternative to the widely used thiol-reactive coumarin dye CPM (#91010).
ROX maleimide, 5 and 6 isomers
5(6)-ROX maleimide has C6-linker that enhances the fluorescence after the dye is conjugated to proteins. The product is a direct replacement of 5(6)-carboxy-X-rhodamine C5-maleimide (91041).
N-(1,10-Phenanthrolin-5-yl)iodoacetamide
N-(1,10-Phenanthrolin-5-yl)iodoacetamide has been used to label thiols to confer the metal-binding properties of the phenanthroline chelator. For example, completing of the chelator with copper can be used to selectively cleave RNA or DNA in the presence of hydrogen peroxide.
Tetramethylrhodamine maleimide, 5 and 6 isomers
5(6)-Carboxytetramethylrhodamine maleimide has C6-linker that enhances the fluorescence after the dye is conjugated to proteins. The product is a direct replacement for 5(6)-carboxytetramethylrhodamine C5-maleimide (91040).
MTS-6-TAMRA
MTS-6-TAMRA (6-carboxytetramethylrhodamine methanethiosulfonate) is a fast-reacting and highly selective thiol-reactive label that permits one to attach the popular fluorescent dye TAMRA to a thiol group.
MTS-5-TAMRA
MTS-5-TAMRA (5-carboxytetramethylrhodamine methanethiosulfonate) is a fast-reacting and highly selective thiol-reactive label that permits one to attach the popular fluorescent dye TAMRA to a thiol group.
DTT
DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.
N-(6-Aminohexyl)maleimide, trifluoroacetic acid salt
N-(6-Aminohexyl)maleimide is a cross-linking reagent that can react with an amine-reactive group and a thiol group.
TCEP
TCEP hydrochloride is an odorless reducing agent that is more stable and effective than dithiothreitol (DTT) or 2-Mercaptoethanol. TCEP hydrochloride is soluble in water to 310 grams per liter.
ABD-F
ABD-F is a useful reagent for the sensitive and specific detection of thiols both by TLC and HPLC (1,2). When combined with a reducing agent such as tributylphosphine, it can also be used for the determination of disulfides in peptides and proteins (3,4).
MTS-Sulforhodamine 101 (Texas Red®)
MTS-sulforhodamine 101 is a fast-reacting and highly selective thiol-reactive rhodamine dye.
3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate
3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.
BisEA
BisEA (Bis-(2-(Methanethiosulfonato)ethyl)amine, hydrochloride) is a positively charged MTS cross-linker.
3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate
3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.
MTS-Rhodamine 6G
MTS-Rhodamine 6G is a fast-reacting and highly selective thiol-reactive rhodamine dye.