Thiol-Reactive Dyes
Maleimide reacts with thiol groups to form thioether-coupled products. The reaction can take place at pH 7 in the presence of amines. At neutral pH, the maleimide group does not react with histidine or arginine. Maleimide labeling can be a useful alternative to succinimidyl ester labeling in cases where modification of lysines alters antibody binding affinity or other biological activity of the target protein. We offer more than 20 bright and photostable CF® Dye Maleimides.
Methanethiosulfonate (MTS) is a fast-reacting and highly selective thiol-reactive functional group. We offer CF® Dye MTS with a selection of popular colors. We also offer MTS thiol-modifying and cross-linking agents.
We also offer many classic thiol-reactive dyes and biotin, as well as reducing agents commonly used in thiol conjugation protocols.
CF® Dye MTS
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CF® dye MTS are fast-reacting, highly selective thiol-reactive derivatives for reversible labeling of thiol groups with fluorescent CF® dyes.
CF® Dye Maleimides
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CF® Dye Maleimides are thiol-reactive fluorescent dyes. Maleimides are commonly used to label proteins, peptides or other molecules containing free thiol groups.
3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate
3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.
3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate
3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.
3,6-Dioxaoctane-1,8-diyl dimethanethiosulfonate
3,6-dioxaoctane-1,8-diyl dimethanethiosulfonate is a water soluble MTS cross-linker.
ABD-F
ABD-F is a useful reagent for the sensitive and specific detection of thiols both by TLC and HPLC (1,2). When combined with a reducing agent such as tributylphosphine, it can also be used for the determination of disulfides in peptides and proteins (3,4).
AEAETS
AEAETS (2-Aminoethyl 2-aminoethanethiosulfonate dihydrochloride salt) is a doubly positively charged alkanethiosulfonate.
Biotin TS Ethylenediamine
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TS biotin ethylenediamine (Biotin ethylenediamine thiosulfate, sodium salt) is a highly selective thiol labeling probe. Similar to the MTS group, TS (thiosulfate) group is capable of reacting with thiols at nearly neutral pH.
BisEA
BisEA (Bis-(2-(Methanethiosulfonato)ethyl)amine, hydrochloride) is a positively charged MTS cross-linker.
Carboxyrhodamine 110-C6-Maleimide, 5 and 6 isomers
CR110-C6-maleimide (full name: 5(6)-Carboxyrhodamine 110-C6-maleimide) is a maleimide derivative of the green fluorescent dye carboxyrhodamine 110.
CFQ520 maleimide
The thiol-reactive quencher, CFQ520 maleimide, has a broad and intense absorption (approximately 520 nm) but no fluorescence, making it useful as an acceptor in fluorescence resonance energy transfer (FRET) applications.
CPM
CPM is a widely used blue fluorescent thiol-reactive dye that is essentially nonfluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step.
CPMTS
Biotium developed CPMTS as an alternative to the widely used thiol-reactive coumarin dye CPM (#91010).
DTT
DTT (dithiothreitol) is a reducing agent commonly used to reduce cystine disulfides to free thiols.
Eosin-5-maleimide
Eosin-5-maleimide is a good photosensitizer and can be used to selectively label thiols. It has a quantum yield of 0.
Fluorescein-5-maleimide
Fluorescein-5-maleimide is the most widely used green fluorescent thiol-reactive dye. The reaction requires very mild conditions and is highly specific.