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Can cells be fixed after CellBrite™ membrane staining? Can CellBrite™ membrane stains be used to stain cells or tissues after they are fixed?

Cells can be fixed with formaldehyde after labeling with original CellBrite™ or CellBrite™ NIR dyes. Lipophilic carbocyanine dyes like the CellBrite™ and CellBrite™ dyes have also been used to stain cells or tissues after formaldehyde fixation. Permeabilization of cells with detergents or solvents, or mounting medium containing glycerol may adversely affect staining. Permeabilization with digitonin (10 ug/mL to 1 mg/mL) has been reported to be compatible with lipophilic carbocyanine dye staining. We’ve seen good results when formaldehyde-fixed cells are permeabilized before staining with CellBrite™ dyes.

CellBrite™ Fix Membrane Stains and MemBrite™ Fix Cell Surface Stains belong to a new class of membrane dyes designed to covalently label the cell surface. They can withstand fixation and permeabilization, or fixation with alcohol after labeling of live cells. CellBrite™ Fix and MemBrite™ Fix cannot be used to label the plasma membranes of fixed cells or tissues (the dyes label the cytoplasm in fixed cells).

To find the right dye for your workflow, see our Comparison of Membrane & Cell Surface Stains, or download our Membrane & Surface Stains Brochure.

Category: CellBrite™ & MemBrite™ Membrane & Cell Surface Stains

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