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Can cells be fixed after CellBrite membrane staining? Can CellBrite membrane stains be used to stain cells or tissues after they are fixed?

Cells can be fixed with formaldehyde after labeling with CellBrite dyes. Lipophilic carbocyanine dyes like the CellBrite dyes have also been used to stain cells or tissues after formaldehyde fixation. Permeabilization of cells with detergents or solvents, or mounting medium containing glycerol may adversely affect staining. Permeabilization with digitonin (10 ug/mL to 1 mg/mL) has been reported to be compatible with lipophilic carbocyanine dye staining.

Category: CellBrite™ Cytoplasmic Membrane Stains

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