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Can cells be fixed after CellBrite™ membrane staining? Can CellBrite™ membrane stains be used to stain cells or tissues after they are fixed?

Cells can be fixed with formaldehyde after labeling with original CellBrite™ or CellBrite™ NIRhttps://biotium.com/product/cellbrite-nir-cytoplasmic-membrane-dye/ dyes. Lipophilic carbocyanine dyes like the CellBrite™ and CellBrite™ dyes have also been used to stain cells or tissues after formaldehyde fixation. Permeabilization of cells with detergents or solvents, or mounting medium containing glycerol may adversely affect staining. Permeabilization with digitonin (10 ug/mL to 1 mg/mL) has been reported to be compatible with lipophilic carbocyanine dye staining.

CellBrite™ Fix Membrane Stains are a new class of membrane dyes that covalently label the cell surface. They can withstand fixation and permeabilization, or fixation with alcohol after labeling of live cells. CellBrite™ Fix cannot be used to label the plasma membranes of fixed cells or tissues (the dyes label intracellular membranes in fixed cells).

Category: CellBrite™ Cytoplasmic Membrane Stains

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