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Dilinoleyl DiO

Dilinoleyl DiO (1,1′-dilinoleyl-3,3′-oxacarbocynanine perchlorate), also known as FAST DiO™, has identical absorption and emission spectra as DiO (#60011).

Product Attributes

Cellular localization

Membrane/cell surface, Membrane/vesicular

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Co-cultures, Extended staining (several days to weeks)

Fixation options

Permeabilize before staining, Fix after staining (formaldehyde), Fix before staining (formaldehyde)

Colors

Green

Excitation/Emission

484/499 nm

Size
Catalog #
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Qty
5 mg
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Product Description

Dilinoleyl DiO (1,1′-dilinoleyl-3,3′-oxacarbocynanine perchlorate), also known as FAST DiO™, has identical absorption and emission spectra as DiO (#60011). However, due to its unsaturated hydrocarbon chains, dilinoleyl DiO has a much faster lateral diffusion rate in cell membranes than DiO.

  • λExEm (MeOH) = 484/499 nm
  • ε = 140,000
  • Yellow orange solid soluble in ethanol, DMF or DMSO
  • Store at -20°C
  • C53H77ClN2O6
  • MW: 873.65

Fast DiO is a trademark of Thermo Fisher Scientific.

References

1. Neuron 17, 91 (1996).

 

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