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Neuro-DiO

Biotium developed Neuro-DiO as a replacement for the green fluorescent DiO, which has been found to be difficult to use for neurons and cell suspension due to low solubility, tendency to form aggregates and slow lateral diffusion rate.

Product Attributes

Cellular localization

Membrane/cell surface, Membrane/vesicular

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Co-cultures, Extended staining (several days to weeks)

Fixation options

Permeabilize before staining, Fix after staining (formaldehyde), Fix before staining (formaldehyde)

Colors

Green

Excitation/Emission

484/501 nm

Size
Catalog #
price
Qty
25 mg
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Product Description

Biotium developed Neuro-DiO as a replacement for the green fluorescent DiO, which has been found to be difficult to use for neurons and cell suspension due to low solubility, tendency to form aggregates and slow lateral diffusion rate. Neuro-DiO and DiO have nearly identical absorption and emission spectra, but the former has better solubility in membranes and does not form nonfluorescent aggregates, which also tend to slow down the dye diffusion rate in membranes. We also offer Neuro-DiO in Vegetable Oil for microinjection studies, and in our CellBrite™ Green Cytoplasmic Membrane Dye, a ready-to-use dye solution for cell labeling.

  • λExEm = 484/501 nm
  • Yellow-orange solid soluble at 1-2 mM (with heating) in DMF, DMSO, or ethanol
  • Soluble at 1-2 mM in vegetable oil with heating and sonication
  • Store at 4°C, protect from light
  • C67H105ClN2O5S
  • MW: 1086

 

References

n/a

 

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