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DiO, also called DiOC18(3), is a green fluorescent, lipophilic carbocyanine dye that is widely used as a lipophilic tracer.

Product Attributes

CAS number


Cellular localization

Membrane/cell surface, Membrane/vesicular

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Co-cultures, Extended staining (several days to weeks)

Fixation options

Permeabilize before staining, Fix after staining (formaldehyde), Fix before staining (formaldehyde)




484/501 nm

Catalog #
50 mg
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Product Description

DiO, also called DiOC18(3), is a green fluorescent, lipophilic carbocyanine dye that is widely used as a lipophilic tracer. This membrane dye has applications similar to those of DiI (#60010). However, DiO has green fluorescent emission and the lateral diffusion rate on the membranes is generally slower than that of DiI. DiO and DiI are often used together in dual color studies.

  • λEx/λEm (MeOH) = 484/501 nm
  • ε (MeOH) = 150,000
  • Yellow orange solid soluble (with heating) at 2 mM in DMF, 1 mM in DMSO, or 2 mM in 1:1 DMSO:ethanol
  • Store at 4°C and protect from light, especially in solution
  • C53H85ClN2O6
  • MW: 882
  • Cas number: 34215-57-1
  • Cas name: Benzoxazolium, 3-octadecyl-2-[3-(3-octadecyl-2(3H)-benzoxazolylidene)-1-propenyl]-, perchlorate


Note: DiO has low solubility, a tendency to form aggregates and slow lateral diffusion rate. See our DiO alternative Neuro-DiO (60015), which is more soluble in membranes and does not form non-fluorescent aggregates. We also offer CellBrite™ Green (30021), a ready-to-use Neuro-DiO solution for cell membrane labeling, and Neuro-DiO in Vegetable Oil (60019) for microinjection studies.


1. Biochemistry 19, 6050 (1980)
2. J. Immunology 127, 893 (1981)
3. Trends Neurosci 9, 333 (1989)
4. J Cell Biol 103, 171 (1986)


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