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DiO, also called DiOC18(3), is a green fluorescent, lipophilic carbocyanine dye that is widely used as a lipophilic tracer.

Product Attributes

CAS number


Cellular localization

Membrane/cell surface, Membrane/vesicular

For live or fixed cells

For live/intact cells, For fixed cells

Assay type/options

Co-cultures, Extended staining (several days to weeks)

Fixation options

Permeabilize before staining, Fix after staining (formaldehyde), Fix before staining (formaldehyde)



Catalog #
50 mg
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Product Description

DiO, also called DiOC18(3), is a green fluorescent, lipophilic carbocyanine dye that is widely used as a lipophilic tracer. This membrane dye has applications similar to those of DiI (#60010). However, DiO has green fluorescent emission and the lateral diffusion rate on the membranes is generally slower than that of DiI. DiO and DiI are often used together in dual color studies. Also see our Neuro-DiO (#60015).

  • λEx/λEm (MeOH) = 484/501 nm
  • ε (MeOH) = 150,000
  • Yellow orange solid soluble in ethanol
  • Store at 4°C and protect from light, especially in solution
  • C53H85ClN2O6
  • MW: 882
  • Cas number: 34215-57-1
  • Cas name: Benzoxazolium, 3-octadecyl-2-[3-(3-octadecyl-2(3H)-benzoxazolylidene)-1-propenyl]-, perchlorate


1. Biochemistry 19, 6050 (1980)
2. J. Immunology 127, 893 (1981)
3. Trends Neurosci 9, 333 (1989)
4. J Cell Biol 103, 171 (1986)


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