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Ethidium Homodimer I, 2 mM in DMSO

Ethidium Homodimer I, first developed by Dr. Le Pecq and his colleagues, is a high affinity fluorescent nucleic acid stain.

Product Attributes

CAS number

61926-22-5

Cellular localization

Nucleus & cytoplasm

Assay type/options

No-wash staining

Cell permeability

Membrane impermeant

Apoptosis/viability marker

Dead cell stain

Colors

Red

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Product Description

Ethidium Homodimer I, first developed by Dr. Le Pecq and his colleagues, is a high affinity fluorescent nucleic acid stain. It binds to both DNA and RNA in a sequence-independent manner and with a >30-fold fluorescence enhancement. The DNA binding of each Ethidium Homodimer covers four base pairs and is believed to occur by intercalation. Because the dye is highly positively charged, it cannot cross cell membranes to stain living cells. It is useful for detecting nucleic acids in solution, or for selectively staining dead cells with damaged plasma membranes. Biotium also Ethidium Homodimer I in solid form (catalog no. 40010).

  • λExEm (with DNA) = 528/617 nm
  • λAbs (in H2O, no DNA) = 493 nm
  • Red solution
  • Store at 4°C and protect from light
  • MW: 857

References

n/a

 

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