ExoBrite™ EV Total RNA Isolation Kit

Obtaining high yields of quality RNA from extracellular vesicles (EVs) is a challenge due the dilute nature of EV samples and low RNA content within EVs. In addition, short non-coding RNAs within EVs, such as miRNAs, are commonly studied as biomarkers and often require specialized methods for efficient extraction. The ExoBrite™ EV Total RNA Isolation Kit was designed to address these challenges by offering an optimized and easy-to-use kit for total RNA isolation, including mRNA and miRNA, from purified EVs. The isolated EV RNA can then be used for downstream analysis such as qPCR or RNAseq.

The RNA isolation procedure is a simple column purification method that takes as little as 20 minutes and requires no phenol/chloroform or ethanol precipitation steps. The kit is estimated to recover
~10-20 ng of RNA from 1×10¹⁰ SEC-enriched EVs. The amount of RNA recovered will depend primarily on EV number and quality, which may be affected by purification method, storage conditions, and number of freeze thaws. An optional, but recommended, DNase treatment step is used to remove contaminating DNA.

See below our full catalog of ExoBrite™ stains and antibodies developed for EV analysis by flow cytometry, western blotting, or super-resolution imaging.

Full List of ExoBrite™ Products for EV Research