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LipidSpot™ Lipid Droplet Stains

LipidSpot™ dyes rapidly stain lipid droplets in live cells or fixed cells, with no wash step required. Available with green or red/far-red fluorescence.

Product Attributes

Dye

LipidSpot™ 488, LipidSpot™ 610

Cellular localization

Lipid droplets

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Fixation options

Fix after staining (formaldehyde), Permeabilize after staining, Fix before staining (formaldehyde)

Colors

Green, Far-red

Concentration

1000X in DMSO

Storage Conditions

Store at 2 to 8 °C, Protect from light

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Technology

Dye
Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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125 uL
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Product Description

LipidSpot™ stains are fluorescent dyes that rapidly stain lipid droplets in live or fixed cells, with no wash step and minimal background.

  • Rapidly and specifically stain lipid droplets
  • Minimal background, no wash required
  • Stain live or fixed cells, or fix and permeabilize after staining
  • Available with green or red/far-red fluorescence
  • LipidSpot™ 488 validated in super-resolution imaging by SIM
  • Supplied at 1000X in DMSO

Intracellular lipid droplets are cytoplasmic organelles involved in the storage and regulation of triglycerides and cholesterol esters. LipidSpot™ dyes are fluorogenic neutral lipid stains that rapidly accumulate in lipid droplets, where they become brightly fluorescent. The dyes can be used to stain lipid droplets in both live and fixed cells, with no wash step required. Cells also can be fixed and permeabilized after staining. LipidSpot™ stains show minimal background staining of cellular membranes or other organelles, unlike traditional dyes like Nile Red.

Find the Right Stain for Your Application

LipidSpot™ 488 has excitation around 430 nm, and can be excited equally well at 405 nm or 488 nm. In cells, it stains lipid droplets with bright green fluorescence detectable in the FITC channel. LipidSpot™ 488 has been validated in super-resolution imaging by SIM (Ref. 3).

LipidSpot™ 610 has excitation/emission at ~592/638 nm in cells; it is optimally detected in the Texas Red® channel, but is also bright in the Cy®3 and far-red Cy®5 channels. Therefore, we don’t recommend pairing LipidSpot™ 610 with other red or far-red probes.

In yeast, LipidSpot™ 488 stains intracellular membranes, but LipidSpot™ 610 does not. In bacteria, both LipidSpot™ dyes can stain gram-positive but not gram-negative strains. See our Cellular Stains Table for more information on how our dyes stain various organisms.

LipidSpot™ Lipid Droplet Stains

LipidSpot™ StainAbs/EmDetection channelCatalog no.Size (1000X
in DMSO)
LipidSpot™ 488427/585 nm
(in vegetable oil or cells)
FITC, GFP70065-T20 uL
70065125 uL
LipidSpot™ 610610/663 nm (in vegetable oil)
~592/638 nm (in cells)
Texas Red®
or Cy®5
70069-T20 uL
70069125 uL
Cy Dye is a registered trademark of GE Healthcare; Texas Red is a registered trademark of Thermo Fisher Scientific.

References

1. Acta Histochem (2019) 121(5), 611-618. doi:10.1016/j.acthis.2019.04.012
2. Cells (2019) 8(2), 182. doi:10.3390/cells8020182
3. Light Sci Apps (2020) 9, 11. https://doi.org/10.1038/s41377-020-0249-4
4. Nat Microbiol (2020). https://doi.org/10.1038/s41564-020-0701-5

 

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