Cholera Toxin Subunit B CF® Dye Conjugates
Binds GM1 ganglioside in lipid rafts and is used as a retrograde neuronal tracer for live imaging or on fixed cells.
Cholera toxin B subunit is used for tract-tracing in neurological research, targeting GM1 ganglioside binding and retrograde transport.
- Choice of 11 bright & photostable CF® Dyes from green to near-infrared
- Fluorescent lipid raft markers and retrograde neuronal tracers for live imaging or on fixed cells
Cholera toxin is the symptom-causing toxin produced by the bacteria Vibrio cholerae during cholera infection. The toxin is composed of two subunits, A and B. Subunit A is the toxic enzymatic subunit present in one copy per toxin. Cholera toxin subunit B (CT-B) is the receptor binding subunit that is found as a pentamer in each toxin and is relatively non-toxic, making it useful for cell biological studies. CT-B has been used as a neuronal tracer and has also been shown to bind to GM1 gangliosides that are found in lipid rafts on the surface of mammalian cells. Therefore, fluorescently labeled conjugates of CT-B have been used as lipid raft markers and endocytic tracers for live imaging or on fixed cells.
Superior CF® Dyes
Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.
Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.
CF® Dye Cholera Toxin Conjugates
|Conjugation||Ex/Em||Size||Catalog No.||Dye Features|
|CF®405M||408/452 nm||100 ug||00068||CF®405M Features|
|CF®488A||490/515 nm||100 ug||00070||CF®488A Features|
|CF®532||527/558 nm||100 ug||00074||CF®532 Features|
|CF®543||541/560 nm||100 ug||00075||CF®543 Features|
|CF®568||562/583 nm||100 ug||00071||CF®568 Features|
|CF®594||593/614 nm||100 ug||00072||CF®594 Features|
|CF®633||630/650 nm||100 ug||00077||CF®633 Features|
|CF®640R||642/662 nm||100 ug||00073||CF®640R Features|
|CF®647||650/665 nm||100 ug||00069||CF®647 Features|
|CF®660R||663/682 nm||100 ug||00078||CF®660R Features|
|CF®680R||680/701 nm||100 ug||00079||CF®680R Features|
CF®568 Cholera Toxin (00071)
Nature (2016) 529, 408–412. https://doi.org/10.1038/nature16516
Nature (2017) 546, 492–497. https://doi.org/10.1038/nature22818
Download a list of CF® dye references.