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Cholera Toxin Subunit B CF® Dye Conjugates

Binds GM1 ganglioside in lipid rafts and is used as a retrograde neuronal tracer for live imaging or on fixed cells.

Product Attributes

Probe cellular localization

Membrane/cell surface

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

Real-time imaging

Detection method/readout

Fluorescence microscopy, Flow cytometry

Cell permeability

Membrane impermeant

Fixation options

Fix after staining (formaldehyde), Fix before staining (formaldehyde)

Toxin

Cholera toxin

Colors

Blue, Green, Orange, Red, Far-red, Near-infrared

Conjugation
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Size
Catalog #
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Qty
100 ug
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Product Description

Cholera toxin B subunit is used for tract-tracing in neurological research, targeting GM1 ganglioside binding and retrograde transport.

  • Choice of 11 bright & photostable CF® Dyes from green to near-infrared
  • Fluorescent lipid raft markers and retrograde neuronal tracers for live imaging or on fixed cells

Cholera toxin is the symptom-causing toxin produced by the bacteria Vibrio cholerae during cholera infection. The toxin is composed of two subunits, A and B. Subunit A is the toxic enzymatic subunit present in one copy per toxin. Cholera toxin subunit B (CT-B) is the receptor binding subunit that is found as a pentamer in each toxin and is relatively non-toxic, making it useful for cell biological studies. CT-B has been used as a neuronal tracer and has also been shown to bind to GM1 gangliosides that are found in lipid rafts on the surface of mammalian cells. Therefore, fluorescently labeled conjugates of CT-B have been used as lipid raft markers and endocytic tracers for live imaging or on fixed cells.

Superior CF® Dyes

Biotium’s next-generation CF® Dyes were designed to be highly water-soluble with advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Learn more about CF® Dyes.

Note: Conjugates of blue-fluorescent dyes like CF®350, CF®405S and CF®405M are not recommended for detecting low abundance targets and may be challenging to use in tissue specimens. Blue dyes have lower fluorescence and photostability, and cells and tissue have high autofluorescence in blue wavelengths, resulting in lower signal to noise compared to other colors.

CF® Dye Cholera Toxin Conjugates

ConjugationEx/EmSizeCatalog No.Dye Features
CF®405M408/452 nm100 ug00068CF®405M Features
CF®488A490/515 nm100 ug00070CF®488A Features
CF®532527/558 nm100 ug00074CF®532 Features
CF®543541/560 nm100 ug00075CF®543 Features
CF®568562/583 nm100 ug00071CF®568 Features
CF®594593/614 nm100 ug00072CF®594 Features
CF®633630/650 nm100 ug00077CF®633 Features
CF®640R642/662 nm100 ug00073CF®640R Features
CF®647650/665 nm100 ug00069CF®647 Features
CF®660R663/682 nm100 ug00078CF®660R Features
CF®680R680/701 nm100 ug00079CF®680R Features
CF is a registered trademark of Biotium, Inc. Alexa Fluor, Texas Red, and DyLight are registered trademarks of Thermo Fisher Scientific.

References

CF®568 Cholera Toxin (00071)
Nature (2016) 529, 408–412. https://doi.org/10.1038/nature16516
Nature (2017) 546, 492–497. https://doi.org/10.1038/nature22818

Download a list of CF® dye references.

 

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