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TMRE, 2 mM in DMSO

TMRE (Tetramethylrhodamine ethyl ester, perchlorate) is used to quantify changes in mitochondrial membrane potential in live cells.

Product Attributes

Cellular localization

Mitochondria

CAS number

115532-52-0

For live or fixed cells

For live/intact cells

Assay type/options

No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Apoptosis/viability marker

Mitochondrial potential

Potential dependence

Mitochondrial potential-dependent

Colors

Red

Excitation/Emission

549/574 nm

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Product Description

TMRE (Tetramethylrhodamine ethyl ester, perchlorate) is a cell permeable, cationic, red-orange fluorescent dye that accumulates active mitochondria. It is used to detect changes in mitochondrial membrane potential of live cells via microplate fluorometry, fluorescence microscopy and flow cytometry. Inactive or depolarized mitochondria have decreased membrane potential and thus fail to retain the TMRE dye.

  • Red-orange fluorescence, λExEm (MeOH) = 549/574 nm
  • Suitable for staining mitochondria in live cells only. It does not work with fixed cells
  • TMRE is used for quantitative measurement of membrane potential using the Nernst equation
  • Store the solution at 4°C and protect from light
  • C26H27ClIN2O7
  • MWt: 515

TMRE and TMRM (70017) are preferred dyes for quantitative measurements of membrane potentials using the Nernst equation. TMRE can be used to separate viable and non-apoptotic cells from cell sorting samples (1). TMRE staining is reversible and does not affect cell proliferation and viability. TMRE is also available in solid form (70016).

 

References

1. J Histochem Cytochem doi: 10.1369/0022155413520404

 

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