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Hoechst

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains.

Product Attributes

CAS number

23491-45-4, 23491-52-3

Cellular localization

Nucleus

For live or fixed cells

For fixed cells, For live/intact cells

Assay type/options

DNA content/cell cycle (flow cytometry), Long term staining (24-72h), No-wash staining, Real-time imaging

Cell permeability

Membrane permeant

Apoptosis/viability marker

All cell stain

Fixation options

Fix after staining (formaldehyde), Fix after staining (methanol), Permeabilize after staining, Fix before staining (methanol), Fix before staining (formaldehyde)

Colors

Blue

Excitation/Emission

350/461 nm (with DNA), 352/461 nm (with DNA)

Dye
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Catalog #
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10 mL (10 mg/mL)
100 mg
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Product Description

Hoechst Products

ProductCatalog NumberMWUnit SizeλEx/λEmFormat
Hoechst 33258, 10 mg/mL in Water40044533.8810 mL352/461 nmYellow solution
Hoechst 33258, pentahydrate40045623.96100 mg352/461 nmYellow solid
Hoechst 33342, 10 mg/mL in Water40046561.9310 mL350/461 nmYellow solution
Hoechst 33342, trihydrochloride trihydrate40047615.98100 mg350/461 nmYellow solid

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. These dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains. Biotium offers Hoechst 33258 and Hoechst 33342 dyes in both solution or powder forms. Both dyes are spectrally similar but Hoechst 33528 is slightly more water soluble than Hoechst 33342. The dyes can be used to stain live or fixed cells in buffer or medium at 1 ug/mL, with no wash step required.

Hoechst can also be used to stain live bacteria (gram-positive and gram-negative), but in live yeast the staining is weak and not nuclear. See our Cellular Stains Table for more information on how our dyes stain various organisms.

Hoechst 33258, pentahydrate

 

Hoechst 33342, trihydrochloride trihydrate

Learn more about Hoechst and our other novel nuclear stains. We also offer DAPI nuclear stains in powder or solution.

Having trouble with your experiment? See our section on troubleshooting tips for fluorescent staining.

References

n/a

 

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