Nuclear Counterstains

Thiazole Green, which is structurally identical to SYBR® Green I Nucleic Acid Gel Stain, is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose, polyacrylamide gels and qPCR.

Thiazole Orange Homodimer (TOhD), also known as TOTO®-1, is a cell-impermeant, high-affinity, fluorogenic green nucleic acid stain.

Thiazole orange (TO) is commonly used in reticulocyte analysis to stain residual RNA of blood cells, to stain DNA in agarose gels and capillary electrophoresis. Thiazole Orange stains the yeast nucleus, and is also able to stain bacteria and mammalian cells.

Thiazole Red, also known as TO-PRO®-3, is a cell-impermeant, high-affinity far-red nucleic acid stain.

Thiazole red homodimer (TRhD), also known as TOTO®-3, is a cell-impermeant, high-affinity, far-red fluorogenic nucleic acid stain.

TO Iodide, also known as TO-PRO®-1, is a green fluorescent, cell-impermeant nucleic acid stain that can be used to stain dead or fixed cells.

DAPI is a popular blue fluorescent DNA dye. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement.

A high purity grade, red fluorescent nucleic acid dye for detecting nucleic acids in solution or for selectively staining dead cells with damaged plasma membranes.

Unique hardset antifade that quenches lipofuscin autofluorescence. Choose with mounting medium with DAPI, far-red NucSpot® 640, or without nuclear stain.

Biotium’s hardset antifade mounting medium with NucSpot® 640 far-red nuclear counterstain.

Oxazole Blue, also known as PO-PRO™-1, is a blue-fluorescent, cell-impermeant nucleic acid stain that can be used to stain dead or fixed cells.

7-AAD (7-aminoactinomycin D) is a membrane-impermeant fluorescent DNA binding dye that is useful for live/dead discrimination and cell cycle profiling by flow cytometry.

Hoechst dyes are cell membrane-permeant, minor groove-binding blue fluorescent DNA stains. Hoechst dyes are widely used in cell cycle and apoptosis studies as nuclear counterstains.

An improved alternative to 7-AAD showing less bleed-through fluorescence in the PE-Texas Red® channel. Ideal for selective detection of dead cells by flow cytometry.

A membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a mixed live-dead population and also used as a counterstain in multicolor fluorescent assays.