Protein Detection & Analysis

The TrueBlack® WB Blocking Buffer Kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).

GloMelt™ dye can be used to detect protein unfolding or measure thermal stability by performing a thermal shift assay, also called Protein Thermal Shift™, differential scanning fluorimetry, or Thermofluor assay.

Mix-n-Stain™ Total Protein Prestain Kits are designed for highly linear protein quantitation on SDS-PAGE gels and western blot membranes via protein labeling with near-infrared CF® dyes. The labeling shows superior linearity for western normalization compared to housekeeping proteins.

One-Step Blue® is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing.

One-Step Lumitein™ Protein Gel Stain is a ready-to-use luminescent protein gel stain. It is a dramatically improved version of our original Lumitein™ protein gel stain for convenience and safety.

One-Step Lumitein™ UV Protein Gel Stain is a ready-to-use luminescent protein gel stain optimized for UV-based fluorescence imaging systems.

Peacock™ Plus Prestained Protein Marker is a visible three-color protein ladder with 12 bands ranging from 8 kDa to 245 kDa.

Peacock™ Prestained Protein Marker is a visible three-color protein ladder with 10 bands ranging from 10 to 180 kDa.

10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

AccuOrange™ Protein Quantitation Kit is a highly sensitive fluorescence-based assay for quantitating purified protein samples in 96-well format (excitation/emission = 480/598 nm). The detection range of the assay is 0.1-15 ug/mL protein.

Nonfat dry milk is commonly added to blocking buffers minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Milk can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.

Fish gelatin is commonly used in blocking solutions to minimize background noise in immunodetection procedures such as Western blotting and immunostaining. Because this gelatin is derived from the skin of cold water fish it does not cross-react with mammalian antibodies.

Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. It can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. The stain combines excellent sensitivity, exceptional user-friendliness and compatibility with common instruments and downstream analysis.

Lumitein™ is a luminescent dye designed for detecting proteins in SDS polyacrylamide (SDS-PAGE) gels. It can also be used to detect proteins in native PAGE gels after an additional SDS incubation step. The stain combines excellent sensitivity, exceptional user-friendliness and compatibility with common instruments and downstream analysis.

Ponceau S is used for the detection of proteins on PVDF and nitrocellulose membranes with a limit of detection of about 250 ng of transferred protein. The dye rapidly stains proteins on membranes pink or light red for easy visual inspection of protein transfer.

Tween® 20 (polysorbate 20) is a polysorbate surfactant commonly used as a blocking and washing agent in Western blotting and other immunostaining assays. Tween® 20 is typically included in blocking buffers and washing buffers at a concentration of 0.1% to reduce non-specific antibody binding.