Reactive CF® Dyes, Other Reactive Dyes & Biotinylation Reagents

CF® Dyes with a tetrazine group react with cyclooctene (TCO, methylcyclopropene) labeled molecules via copper-free click chemistry.

Fluorescent CF® Dye tyramides are used for tyramide signal amplification (TSA) for increasing immunofluorescence sensitivity in multicolor immunocytochemistry (ICC), immunohistochemistry (IHC), or in situ hybridization (ISH).

Easily label SNAP-tag®, CLIP-tag™, HaloTag®, or other ligands with fluorescent dyes, no purification needed. Cell membrane-permeant and impermeant dye options for intracellular or surface targets.

Kits for labeling & purification of near-IR antibody conjugates for small animal in vivo imaging, featuring Biotium’s industry-leading near-IR CF® Dyes.

Biotin with methyltetrazine reacts with cyclooctene (TCO, methylcyclopropene) labeled molecule via copper free click chemistry.

Biotin with TCO (trans-cyclooctene) reacts with tetrazine labeled molecule via copper free click chemistry.

CF®DI Aminooxy derivatives are useful for fluorescently labeling aldehyde/ketone groups on biomolecules such as polysaccharides, glycoproteins or antibodies to generate size- and charge-matched conjugates.

CF®DI dye hydrazides react with carbonyl moieties of aldehyde or ketone groups. They are size- and charge-matched for detection of protein carbonylation by fluorescence two-dimensional differential gel electrophoresis (2D-DIGE).

Cyanine 555 and Cyanine 647 alkynes can be used for the analysis of O-GlcNAcylated proteins by 2D glyco-DIGE.

Cyanine dyes with an aminooxy reactive group are useful for fluorescently labeling aldehyde or ketone groups on target molecules such as polysaccharids, glycoproteins or antibodies.

Biotium offers high quality amine-reactive succinimidyl ester (SE) dyes used to label proteins, oligonucleotides, and nucleic acids. We offer Cyanine 555, Cyanine 647, Cyanine 680, and Cyanine 750 as high quality succinimidyl esters.

MB (methylene blue) acid can be conjugated to peptides, proteins, drugs, polymeric materials and biomolecules with primary amine groups.  The conjugate will have a blue color and be able to complex with nucleic acids.

CF® Dye with dibenzocyclooctyne (i.e., DBCO, ADIBO) reacts with azide via copper-free click chemistry by strain-promoted azide-alkyne cycloaddition (SPAAC).

A DNP moiety containing a PEG linker and an NHS ester terminal group which can be used for labeling biomolecules with a DNP hapten tag. The NHS ester will react with primary amines to form a stable amide bond while the hydrophilic PEG linker improves the solubility of the labeled bioconjugate.

Label your antibody with one of our CF® Dyes specialized for STORM super-resolution microscopy. Labeling takes just 30 minutes without a purification step, and yields an antibody conjugate with a low degree of labeling optimal for STORM.