Western Blotting Reagents

Buffer system optimized for fluorescence-based western provides superior specificity, sensitivity, and background suppression.

A solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific antibody binding in immuno-detection procedures such as western blotting and cell staining.

Can be used as a stabilizing agent for proteins and enzymes, or added to blocking buffers to minimize non-specific antibody binding in immuno-detection procedures such as western blotting and cell staining.

Can be used as a stabilizing agent for proteins and enzymes, or added to blocking buffers to minimize non-specific antibody binding in immuno-detection procedures such as western blotting and cell staining.

DAB reacts with HRP in the presence of peroxide to yield an insoluble brown-colored product at locations where peroxidase-conjugated antibodies are bound to samples.

A blocking agent commonly used to minimize background noise in immunodetection procedures such as western blotting and immunostaining.

Gelatin from fish skin that can be added to blocking buffers to minimize non-specific antibody binding in immuno-detection procedures such as western blotting and cell staining.

A red visible dye that rapidly stains proteins on western blotting membranes for easy visual inspection of protein transfer.

A polysorbate surfactant commonly used as a blocking and washing agent in Western blotting and other immunostaining assays.

Suitable for use in molecular biology applications that require high quality, ultra-pure water.

A convenient 4X sample loading buffer for protein gel electrophoresis. The loading buffer is optimized for use with denaturing SDS-PAGE.

Kits for reversible protein visualization and quantitation on protein gels and western blot membranes by labeling with near-infrared CF® dyes.

A pink derivative of BCIP that produces a pink colored (λ_max 540 nm) precipitate instead of the dark blue precipitate of BCIP. BCIP and derivatives are the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins and are compatible with a variety of applications.

NBT and BCIP PINK are widely used together as a chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications, such as immunohistochemistry, westerns, and in situ hybridization. This kit contains 100 mg each of BCIP Pink (10006) and the precipitation inducer NBT (10008).

A red derivative of BCIP that produces a red (λ_max 565 nm) colored precipitate instead of the blue/violet precipitate of BCIP. BCIP and derivatives are the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins and are compatible with a variety of applications.

A red derivative of BCIP that produces a red (λ_max 565 nm) colored precipitate instead of the dark blue precipitate of BCIP. BCIP and derivatives are the most widely used chromogenic phosphatase substrate for the detection of alkaline phosphatase labeled proteins in a variety of applications.