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MemBrite™ Fix Cell Surface Stains

Options for super-resolution imaging

MemBrite™ Fix Cell Surface Staining Kits provide a convenient way to visualize cell boundaries in multicolor staining experiments. Staining is rapid and uniform, with a wide choice of colors. MemBrite™ Fix dyes covalently label the surface of live cells. The staining withstands fixation and permeabilization for subsequent immunofluorescence staining.

Several MemBrite™ Fix dyes have been validated in super-resolution imaging applications or 2-photon microscopy. MemBrite™ Fix-ST dyes are recommended for super-resolution imaging by STORM. MemBrite™ Fix or MemBrite™ Fix-ST dyes can be used for standard microscopy applications; however, MemBrite™ Fix dyes are generally more photostable than MemBrite™ Fix-ST dyes. See the table below for more information.

MemBrite™ Fix Cell Surface Staining Kits

Catalog numberSize1Dye2Spectrally
similar to
Specialized
applications
30092-T100 reactionsMemBrite™ Fix 405/430Alexa Fluor® 405
CellBrite™ Blue
SIM
30092500 reactions
30093-T100 reactionsMemBrite™ Fix 488/515FITC
Alexa Fluor® 488
DiO
CellBrite™ Green
STED
TIRF
2-photon microscopy
30093500 reactions
30094-T100 reactionsMemBrite™ Fix 543/560TAMRA
Cy®3
Alexa Fluor® 546
DiI
CellBrite™ Orange
N/A
30094500 reactions
30095-T100 reactionsMemBrite™ Fix 568/580Alexa Fluor® 568
Rhodamine Red
DiI
CellBrite™ Orange
SIM
STORM
TIRF
30095500 reactions
30096-T100 reactionsMemBrite™ Fix 594/615Texas Red®
Alexa Fluor® 594
2-photon
microscopy
30096500 reactions
30097-T100 reactionsMemBrite™ Fix 640/660Cy®5,
Alexa Fluor® 647
DiD
CellBrite™ Red
FLImP
SIM
TIRF
30097500 reactions
30098-T100 reactionsMemBrite™ Fix 660/680Alexa Fluor® 660N/A
30098500 reactions
30099-T100 reactionsMemBrite™ Fix 680/700Cy®5.5
Alexa Fluor® 680
IRDye® 680LT
CellBrite™ NIR 680
STORM3
Single-molecule imaging
STED
2-photon microscopy
30099500 reactions
30101-T100 reactionsMemBrite™ Fix-ST 650/665Cy®5
Alexa Fluor® 647
DiD
CellBrite™ Red
STORM
30101500 reactions
30102-T100 reactionsMemBrite™ Fix-ST 667/685Alexa Fluor® 660STORM
30102500 reactions
30103-T100 reactionsMemBrite™ Fix-ST 681/698Cy®5.5
Alexa Fluor® 680
IRDye® 680LT
CellBrite™ NIR 680
Single-molecule imaging
STORM
30103500 reactions
30104-T100 reactionsMemBrite™ Fix-ST 755/777Alexa Fluor® 750
Cy®7
DyLight® 750
STORM
30104500 reactions
FLImP: Fluorophore localization imaging with photobleaching; SIM: Structured illumination microscopy; STED: Stimulated emission depletion; STORM: Stochastical optical reconstruction microscopy; TIRF: Total internal reflection fluorescence

1. Kit size based on 200 uL reaction volume, actual number of reactions will depends on staining volume used.
2. MemBrite™ Fix dyes are named for their Abs/Em maxima. Download the Product Information Sheet for spectra.
3. MemBrite™ Fix-ST 681/698 dye is reported to have better performance in STORM imaging than MemBrite™ Fix 680/700 dye.

Alexa Fluor & Texas Red are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of GE Healthcare; IRDye is a registered trademark of LI-COR Bioscience.

MemBrite™ Fix Cell Surface Staining Kits

Sizes: 100 labelings, 500 labelingsCatalog #: 30092, 30092-T, 30093, 30093-T, 30094, 30094-T, 30095, 30095-T, 30096, 30096-T, 30097, 30097-T, 30098, 30098-T, 30099, 30099-T, 30101, 30101-T, 30102, 30102-T, 30103, 30103-T, 30104, - 30104-TView allHide

Cell Surface & Membrane Dyes

Classic membrane and membrane potential dyes, as well as unique dyes for covalent labeling of the cell surface.

Cell & Organelle Stains

We offer a wide selection of fluorescent organelle stains and other probes for live or fixed cells.