MemBrite™ Fix Cell Surface Staining Kits provide a convenient way to visualize cell boundaries in multicolor staining experiments. Staining is rapid and uniform, with a wide choice of colors. MemBrite™ Fix dyes covalently label the surface of live cells. The staining withstands fixation and permeabilization for subsequent immunofluorescence staining.
Several MemBrite™ Fix dyes have been validated in super-resolution imaging applications or 2-photon microscopy. MemBrite™ Fix-ST dyes are recommended for super-resolution imaging by STORM. MemBrite™ Fix or MemBrite™ Fix-ST dyes can be used for standard microscopy applications; however, MemBrite™ Fix dyes are generally more photostable than MemBrite™ Fix-ST dyes. See the table below for more information.
MemBrite™ Fix Cell Surface Staining Kits
| Catalog number | Size1 | Dye2 | Spectrally similar to | Specialized applications |
|---|---|---|---|---|
| 30092-T | 100 reactions | MemBrite™ Fix 405/430 | Alexa Fluor® 405 CellBrite™ Blue | SIM |
| 30092 | 500 reactions | |||
| 30093-T | 100 reactions | MemBrite™ Fix 488/515 | FITC Alexa Fluor® 488 DiO CellBrite™ Green | STED TIRF 2-photon microscopy |
| 30093 | 500 reactions | |||
| 30094-T | 100 reactions | MemBrite™ Fix 543/560 | TAMRA Cy®3 Alexa Fluor® 546 DiI CellBrite™ Orange | N/A |
| 30094 | 500 reactions | |||
| 30095-T | 100 reactions | MemBrite™ Fix 568/580 | Alexa Fluor® 568 Rhodamine Red DiI CellBrite™ Orange | SIM STORM TIRF |
| 30095 | 500 reactions | |||
| 30096-T | 100 reactions | MemBrite™ Fix 594/615 | Texas Red® Alexa Fluor® 594 | 2-photon microscopy |
| 30096 | 500 reactions | |||
| 30097-T | 100 reactions | MemBrite™ Fix 640/660 | Cy®5, Alexa Fluor® 647 DiD CellBrite™ Red | FLImP SIM TIRF |
| 30097 | 500 reactions | |||
| 30098-T | 100 reactions | MemBrite™ Fix 660/680 | Alexa Fluor® 660 | N/A |
| 30098 | 500 reactions | |||
| 30099-T | 100 reactions | MemBrite™ Fix 680/700 | Cy®5.5 Alexa Fluor® 680 IRDye® 680LT CellBrite™ NIR 680 | STORM3 Single-molecule imaging STED 2-photon microscopy |
| 30099 | 500 reactions | |||
| 30101-T | 100 reactions | MemBrite™ Fix-ST 650/665 | Cy®5 Alexa Fluor® 647 DiD CellBrite™ Red | STORM |
| 30101 | 500 reactions | |||
| 30102-T | 100 reactions | MemBrite™ Fix-ST 667/685 | Alexa Fluor® 660 | STORM |
| 30102 | 500 reactions | |||
| 30103-T | 100 reactions | MemBrite™ Fix-ST 681/698 | Cy®5.5 Alexa Fluor® 680 IRDye® 680LT CellBrite™ NIR 680 | Single-molecule imaging STORM |
| 30103 | 500 reactions | |||
| 30104-T | 100 reactions | MemBrite™ Fix-ST 755/777 | Alexa Fluor® 750 Cy®7 DyLight® 750 | STORM |
| 30104 | 500 reactions |
1. Kit size based on 200 uL reaction volume, actual number of reactions will depends on staining volume used.
2. MemBrite™ Fix dyes are named for their Abs/Em maxima. Download the Product Information Sheet for spectra.
3. MemBrite™ Fix-ST 681/698 dye is reported to have better performance in STORM imaging than MemBrite™ Fix 680/700 dye.
Alexa Fluor & Texas Red are registered trademarks of Thermo Fisher Scientific; Cy Dye is a registered trademark of GE Healthcare; IRDye is a registered trademark of LI-COR Bioscience.