CF®543 Dye

CF®543 is an orange fluorescent dye with absorption and emission maxima at 541 and 560 nm, respectively (Figure 1). The dye is based on a new class of rhodamine dyes invented by Biotium scientists. Unlike traditional rhodamine dyes, CF®543 is not only bright and photostable but also highly water-soluble. As a result, CF®543 antibody conjugates retain excellent solubility and yield the brightest fluorescence among spectrally similar dyes like Alexa Fluor® 546 (Figure 2).

See products available with CF®543. Additional conjugates or reactive dyes may be added upon request; contact techsupport@biotium.com to inquire.

Figure 1. Normalized absorption and emission spectra of CF®543 goat anti-mouse conjugate in PBS.

CF®543 Technical Summary

Abs/Em maxima: 541/560 nm
Extinction coefficient: 100,000
Molecular weight: ~870
Laser line: 532 nm, 543, or 546 nm
Direct replacement for: Alexa Fluor® 546, Tetramethylrhodamine (TAMRA)

Figure 2. CF®543 yields much brighter antibody conjugates than Alexa Fluor® 546. Relative fluorescence of goat anti-mouse conjugates with varying degrees of lab... See More

Figure 3. HeLa cells stained with mouse anti-tubulin and CF®543 goat anti-mouse (microtubules, red), followed by CF®488A Mix-n-Stain™ labeled mouse anti-transfe... See More

Helpful Tools & Resources

Spectra Viewer

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.

Download Our CF® Dye eBook

Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.

CF® Dye Selection Tool

Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.

CF® Dye Reference Guide

Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.