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What dyes can be used to label membranes, to outline cells, in FFPE tissue sections?

The CF® Dye WGA Conjugates can be used to label the cell surface or plasma membrane in FFPE sections, however as these are tissue sections, the lectins would label glycoproteins on all cell membranes, external (plasma membrane) as well as internal (organelle membranes). WGA staining can also be tissue- and cell-type dependent i.e. it is based on the expression pattern of glycoproteins on the cell membranes.

Other cell surface membrane stains such as the lipophilic CellBrite™ dyes are not suitable for membrane staining in FFPE samples as membrane lipids are extracted during the dewaxing and rehydration process. The fixable membrane stains, CellBrite™ fix and MemBrite™ fix are for live cell staining only. In fixed sections, they would indiscriminately react with target proteins throughout the cell, staining the entire cell.

Alternatively, immunostaining using cell surface-specific antibodies could be done.

To find the right dye for your workflow, see our Comparison of Membrane & Cell Surface Stains, or download our Membrane & Surface Stains Brochure.

 

Category: CellBrite™ & MemBrite™ Membrane & Cell Surface Stains

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