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AccuBlue® Broad Range dsDNA Quantitation Solution

A fluorescent dsDNA quantitation solution with a linear range of 2-2000 ng DNA. Designed for use in a fluorescence microplate reader.

Product Attributes


350/460 nm (with DNA)

Storage Conditions

Store at 2 to 8 °C

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
Catalog #
1500 assays
Trial size (200 assays)
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Product Description

The AccuBlue® Broad Range dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants.  The assay provides a linear detection range from 2 – 2000 ng and is designed for use with fluorescence microplate readers. This solution does not come with a DNA standard; see the AccuBlue® Broad Range Kit (31007) which contains a set of calf thymus DNA standards.


  • Highly specific for dsDNA
  • Broad linear detection range, from 2-2000 ng
  • Designed for fluorescence microplate readers
  • Blue fluorescence emission

Kit Components

  • AccuBlue® Broad Range Dye, 100X
  • AccuBlue® Broad Range Buffer, 1X
  • AccuBlue® Broad Range Enhancer, 100X

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA & RNA Quantitation Kits

Kit NameDNA or RNADye Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity DNADNA502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range DNADNA500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen DNADNA468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity DNADNA468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity DNADNA485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range DNADNA350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence
AccuBlue® Broad Range RNARNA650/670Fluorescent Plate Reader or Qubit® fluorometerThe widest linear range of available RNA quantitation kits, with exceptional accuracy, sensitivity, and high RNA selectivity
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit is a registered trademark of Thermo Fisher Scientific.


1. PLoS ONE (2013), Dec 6;8(12) e80613 doi:10.1371/journal.pone.0080613
2. Am J Med Genet B Neuro Genet (2013) 162(B)(4)404-12 doi:10.1002/ajmg.b.32157
3. PLoS ONE (2015), Feb 6;10(2) e0117792 doi:10.1371/journal.pone.0117792
4. PLoS ONE (2015), Jul 6;10(7):e0132137 doi:10.1371/journal.pone.0132137
5. Biotechniques (2018) 64:59-68 doi:10.4155/btn-2017-0102


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