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AccuBlue® Broad Range dsDNA Quantitation Solution

A fluorescent dsDNA quantitation solution with a linear range of 2-2000 ng DNA. Designed for use in a fluorescence microplate reader.

Product Attributes


350/460 nm (with DNA)

Storage Conditions

Store at 2 to 8 °C

Catalog #
1500 assays
Trial size (200 assays)
Clear selection
(Please select quantity above )

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Product Description

The AccuBlue® Broad Range dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants.  The assay provides a linear detection range from 2 – 2000 ng and is designed for use with fluorescence microplate readers. This solution does not come with a DNA standard; see the AccuBlue® Broad Range Kit (31007) which contains a set of calf thymus DNA standards.


  • Highly specific for dsDNA
  • Broad linear detection range, from 2-2000 ng
  • Designed for fluorescence microplate readers
  • Blue fluorescence emission

Kit Components

  • AccuBlue® Broad Range Dye, 100X
  • AccuBlue® Broad Range Buffer, 1X
  • AccuBlue® Broad Range Enhancer, 100X

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA & RNA Quantitation Kits

or RNA
Detection range
(in assay)*
Dye Ex/Em (nm) Suggested instrument Features
High Sensitivity DNA
DNA 0.1-100 ng 502/523 Qubit® fluorometer Compare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range DNA DNA 2-1000 ng 500/530 Qubit® fluorometer Compare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen DNA DNA 1-3000 pg** 468/507 Fluorescence microplate reader Most sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
AccuClear® Ultra High Sensitivity DNA DNA 0.03-250 ng 468/507 Fluorescence microplate reader Versatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity DNA DNA 0.2-100 ng 485/530 Fluorescence microplate reader Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range DNA DNA 2-2000 ng 350/460 Fluorescence microplate reader Broad linear range with blue fluorescence
AccuBlue® Broad Range RNA RNA 5-1000 ng 650/670 Fluorescence microplate reader
or Qubit® fluorometer
The widest linear range of available RNA quantitation kits
Exceptional accuracy, sensitivity, and high RNA selectivity
* Standard assay volume is 200 uL
** AccuBlue® NextGen limit of detection is in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit is a registered trademark of Thermo Fisher Scientific.


1. PLoS ONE (2013), Dec 6;8(12) e80613 doi:10.1371/journal.pone.0080613
2. Am J Med Genet B Neuro Genet (2013) 162(B)(4)404-12 doi:10.1002/ajmg.b.32157
3. PLoS ONE (2015), Feb 6;10(2) e0117792 doi:10.1371/journal.pone.0117792
4. PLoS ONE (2015), Jul 6;10(7):e0132137 doi:10.1371/journal.pone.0132137
5. Biotechniques (2018) 64:59-68 doi:10.4155/btn-2017-0102


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