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AccuBlue® Broad Range dsDNA Quantitation Solution

The AccuBlue® Broad Range dsDNA Quantitation Kit is designed for use with fluorescent plate readers in a 96-well microplate format. The AccuBlue® Broad Range Kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.

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Product Description

The AccuBlue® Broad Range dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants.  The solution provides a linear detection range from 2 – 2000 ng and is compatible with fluorescence microplate readers. This item is a standalone fluorescent DNA quantitation solution, and does not include DNA standards. It is intended for users who wish to use their own DNA standards. We also sell the complete AccuBlue® Broad Range Quantitation Kit that includes a set of standards (catalog no. 31007).

For broad range DNA quantitation using the Qubit® fluorometer from Thermo Fisher, we recommend using our AccuGreen Broad Range dsDNA Quantitation Kit (catalog no. 31069).

For high-sensitivity DNA quantitation using a fluorescent plate reader, we recommend using our AccuClear® or AccuBlue® NextGen dsDNA Quantitation Kits, which provide unprecedented sensitivity, accuracy and wide linear range. See the table below for details on our full line of dsDNA quantitation kits.

Features

  • Broad linear detection range, from 2-2000 ng
  • Blue fluorescence emission (Ex/Em: 350/460 nm)

Components

  • AccuBlue® Broad Range Dye, 100X
  • AccuBlue® Broad Range Buffer, 1X
  • AccuBlue® Broad Range Enhancer, 100X

DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)
Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.

References

1. PLoS ONE (2013), doi:10.1371/journal.pone.0080613

2. J Thromb Thrombolytic (2014), doi: 10.1007/s11239-014-1137-7

3. Molecular Ecology (2015), doi: 10.1111/mec.13167

4. Chem. Res. Toxicol. (2018), doi: 10.1021/acs.chemrestox.8b00050

 

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