Support

Quick links
Frequently asked questions - categories
Ask a question
Contact Us
+1

AccuBlue® Broad Range dsDNA Quantitation Kit with DNA Standards

The AccuBlue® Broad Range dsDNA Quantitation Kit is designed for use with fluorescent plate readers in a 96-well microplate format. The AccuBlue® Broad Range Kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.

Size
Catalog #
price
Qty
1500 assays
Trial size (200 assays)
Clear selection
Add to wishlist
ADD TO CART
(Please select quantity above )

Wishlist updated! View wishlist

Shipping, Handling & Tax (CA only) not included in price. Learn more

Tax is assessed in the state of California. All fees will be calculated during checkout based on your shipping information and are subject to change.

SPECIAL - Order 5 or more and receive 10% off.

Request a Bulk Quote

Product Description

The AccuBlue® Broad Range dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants.  The kit provides a linear detection range from 2 – 2000 ng and is compatible with fluorescence microplate readers. AccuBlue® Broad Range dsDNA Quantitation Solution also is available separately (catalog no. 31009) for customers who wish to use their own DNA standards.

For broad range DNA quantitation using the Qubit® fluorometer from Thermo Fisher, we recommend using our AccuGreen Broad Range dsDNA Quantitation Kit (catalog no. 31069).

For high-sensitivity DNA quantitation using a fluorescent plate reader, we recommend using our AccuClear® or AccuBlue® NextGen dsDNA Quantitation Kits, which provide unprecedented sensitivity, accuracy and wide linear range. See the table below for details on our full line of dsDNA quantitation kits.

Features

  • Broad linear detection range, from 2-2000 ng
  • Blue fluorescence emission (Ex/Em: 350/460 nm)

Kit Components

200 Assays (Trial Size)

  • AccuBlue® Broad Range Dye, 100X
  • AccuBlue® Broad Range Buffer, 1X
  • AccuBlue® Broad Range Enhancer, 100X
  • AccuBlue® dsDNA Standard, 200 ng/uL

1000 Assays

  • AccuBlue® Broad Range Dye, 100X
  • AccuBlue® Broad Range Buffer, 1X
  • AccuBlue® Broad Range Enhancer, 100X
  • AccuBlue® dsDNA Standards, set of 9

 

DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)
Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.

References

1. PLoS ONE (2013), doi:10.1371/journal.pone.0080613

2. J Thromb Thrombolytic (2014), doi: 10.1007/s11239-014-1137-7

3. PLoS ONE (2015), doi:10.1371/journal.pone.0117792

4. Molecular Ecology (2015), doi: 10.1111/mec.13167

5. PLoS ONE (2015), doi:10.1371/journal.pone.0132137

6. Chem. Res. Toxicol. (2018), doi: 10.1021/acs.chemrestox.8b00050

 

 

You may also like…