AccuGreen™ kits are designed for use with the Qubit® fluorometer. All of the other kits are designed for use with 96-well fluorescence plate readers. Our quantitation kits vary in the concentration ranges of dsDNA that they are able to detect. Some of the kits also have different fluorescence excitation/emission. Visit the DNA Quantitation Kit Technology Page or consult the table below to make your selection.
The AccuBlue® Broad Range RNA Quantitation is optimized for use with the Qubit® fluorometer using the pre-programmed RNA Broad Range program. It also can be used with fluorescence microplate readers equipped for detecting far-red fluorescence (Ex/Em 630/660 nm). The kit has a wide linear range of 5-1000 ng RNA, spanning those of both the Quant-iT™ RNA and Quant-iT™ RNA Broad Range Kits from Thermo Fisher Scientific.
The AccuClear® and AccuBlue® DNA Quantitation Kits are designed for use with fluorescence 96-well plate readers. AccuClear®, AccuBlue® NextGen and AccuBlue® High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor®-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kits, which are designed for use on that instrument.
For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2.5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format.
The AccuBlue® Broad Range RNA Quantitation has a wide linear range of 5-1000 ng RNA, spanning those of both the Quant-iT™ RNA and Quant-iT™ RNA Broad Range Kits from Thermo Fisher Scientific. It can be used with fluorescence microplate readers equipped for detecting far-red fluorescence (Ex/Em 630/660 nm), or with the Qubit® RNA Broad Range program.
The AccuGreen™ High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples. The kit provides a linear detection range from 0.1 – 100 ng and is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher Scientific. The AccuGreen™ High Sensitivity assay is also available without a DNA standard (31068), for those that wish to use their own standard.
Designed specifically for Thermo’s Qubit® fluorometer
Linear range: 0.1-100 ng dsDNA
Sample concentration range: 5 pg/uL to 100 ng/uL
Green fluorescence emission
Replaces Qubit® dsDNA HS Assay Kit for cost savings
AccuGreen™ Dye, 200X
DNA Quantitation Buffer, 20X Concentrate*
AccuGreen™ Standard 1 (0 ng/uL)
AccuGreen™ Standard 2 (10 ng/uL)
*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.
AccuGreen™ for the Qubit® fluorometer
The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher Scientific.
The linear range of the AccuGreen™ assay is 0.1 to 100 ng of DNA per tube. DNA samples with concentrations between 5 pg/uL and 100 ng/uL may be quantified using sample volumes between 1 and 20 uL (for example, 1 uL of 100 ng/uL is 100 ng total, and 20 uL of 5 pg/uL is 0.1 ng total, which will both fall within the linear range of the assay). If you use the most common sample volume of 10 uL, the starting sample concentration should be 10 pg/uL to 10 ng/uL.
Fluorescence-Based dsDNA Quantitation
AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.
Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.
Fluorescence microplate reader
or Qubit® fluorometer
The widest linear range of available RNA quantitation kits
Exceptional accuracy, sensitivity, and high RNA selectivity
* Standard assay volume is 200 uL
** AccuBlue® NextGen limit of detection is in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit is a registered trademark of Thermo Fisher Scientific.