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AccuBlue High Sensitivity dsDNA Quantitation Kit with DNA Standards

The AccuBlue™  High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The assay provides a  with sufficient reagents to perform 1000 assays based on a 96-well microplate format.

AccuGreen High Sensitivity dsDNA Quantitation Solution
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31006, - 31006-TView allHide

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Trial size, 1,000 assays

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Product Description

The AccuBlue™  High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The assay provides a  with sufficient reagents to perform 1000 assays based on a 96-well microplate format. AccuBlue™ High Sensitivity Quantitation Solution also is available separately (Catalog #31008) for customers who wish to use their own DNA standards.

The assay is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers. Note that while the assay is spectrally compatible with Qubit® fluorometers from Thermo Fisher, it may not be compatible with the high sensitivity dsDNA program on all Qubit® models (see our DNA Quantitation FAQs for more information). For the Qubit® we recommend our AccuGreen™ High Sensitivity Kit (cat. no. 31066), which was specially formulated for that instrument.

Features

  • Linear detection range from 0.2 – 100 ng
  • λExEm 485/530 nm
  • Safer than PicoGreen® or Quant-iT® dsDNA detection kits: the high sensitivity reagent is impermeable to cell membranes

Kit sizes

  • The 1000 assay size kit includes AccuBlue™ High Sensitivity Quantitation Solution components and a set of 8 pre-diluted calf thymus dsDNA standards.
  • The trial size kit includes one DNA standard solution with instructions for preparing a set of standards by serial dilution.

Other DNA Quantitation Kits:

dsDNA Quantitation KitLinear detection range (dsDNA in assay)Ex/Em (nm)Features
AccuBlue™ NextGen1 pg*-3 ng468/507Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity.
AccuClear™ Ultra High Sensitivity30 pg-250 ng468/507This kit offers great sensitivity and the widest linear range.

AccuBlue™ High Sensitivity0.2-100 ng485/530Quantitation of dsDNA from 0.2-100 ng, with a safer, membrane impermeable DNA dye.
AccuGreen™ High Sensitivity0.1-100 ng502/523Quantitation of DNA using the Qubit® fluorometer from Thermo Fisher.
AccuBlue™ Broad Range2-2000 ng350/460For quantitation of high concentration DNA in a broad linear range with blue fluorescence. Assay can be extended to 4000 ng with minor loss of linearity.

AccuBlue™ technology is covered by granted U.S. and international patents.

References

1. Conservation Genet Resour(2012), doi: 10.1007/s12686-011-9450-3

2. Parasites & Vectors (2012), doi:10.1186/1756-3305-5-59

3. Journal of Chromatography A (2010), doi:10.1016/j.chroma.2013.01.065

4. PLoS ONE (2013), doi:10.1371/journal.pone.0054743

5. Journal of Chromatography A (2013), http://dx.doi.org/doi:10.1016/j.chroma.2013.11.039

6. Journal of Biomechanics (2013), http://dx.doi.org/10.1016/j.jbiomech.2013.10.036

7. Peer J (2013), http://dx.doi.org/10.7717/peerj.203

8. Journal of Biomechanics (2014), http://dx.doi.org/10.1016/j.jbiomech.2013.10.036

9. Analytical Biochemistry (2014),  http://dx.doi.org/10.1016/j.ab.2014.03.005

10. J. Bacteriol. (2014), doi:10.1128/JB.01493-14

11. The International Journal of Biochemistry & Cell Biology (2014), doi: 10.1016/j.biocel.2014.04.026

12. BMC Oral Health (2014), doi: 10.1186/1472-6831-14-157

13. Applications in Plant Sciences (2015), http://dx.doi.org/10.3732/apps.1400077

14. Journal of Biotechnology (2015), http://dx.doi.org/10.1016/j.jbiotec.2015.04.006

15. Conserv Genet (2015), doi: 10.1007/s10592-015-0784-3

16. Am J Physiol Regul Integr Comp Physiol (2015), doi:10.1152/ajpregu.00421.2015

17. Marine Genomics (2015), doi: 10.1016/j.margen.2015.10.010

18. Drury et al. BMC Genomics (2016), doi: 10.1186/s12864-016-2583-8

19. Mitochondrial DNA Part B Resources (2016), http://dx.doi.org/10.1080/23802359.2016.1149787

20. Antonie van Leeuwenhoek (2017), doi: 10.1007/s10482-017-0925-4

 

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    AccuBlue High Sensitivity dsDNA Quantitation Solution

    The AccuBlue™  High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The assay provides a  with sufficient reagents to perform 1000 assays based on a 96-well microplate format.

    AccuClear Ultra High Sensitivity dsDNA Quantitation Kit with 7 DNA Standards

    AccuClear™ Ultra High Sensitivity dsDNA Quantitation Solution utilizes a novel, next-generation DNA binding dye for highly sensitive and accurate quantitation of purified dsDNA samples across a broad range of DNA concentrations. The assay is linear between 0.03 ng and 250 ng of dsDNA per assay in microplate format making it the most versatile DNA quantitation assay.

    AccuBlue Broad Range dsDNA Quantitation Kit with DNA Standards

    The AccuBlue™ Broad Range dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants.  The kit provides a linear detection range from 2 - 2000 ng and is compatible with fluorescence microplate readers.

    AccuBlue NextGen dsDNA Quantitation Kit

    The AccuBlue™ NextGen dsDNA Quantitation Kit provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue™ NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA.

    AccuGreen High Sensitivity dsDNA Quantitation Kit

    The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ kit is specific for dsDNA, and can quantify DNA samples in the range of 0.01-10 ng/uL.

    RNase-Free Calf Thymus DNA, 1 mg/mL

    This double-stranded Calf Thymus DNA is free of RNase activity, and is useful in applications where RNase contamination needs to be avoided. It has been prepared using Biotium's proprietary method to irreversibly inactivate RNase activity, and tested to confirm that there is no RNAse activity remaining.

Support & Faq

DNA Quantitation Kits

AccuGreen™ kits are designed for use with the Qubit® fluorometer. All of the other kits are designed for use with 96-well fluorescence plate readers. Our quantitation kits vary in the concentration ranges of dsDNA that they are able to detect. Some of the kits also have different fluorescence excitation/emission. Please see the AccuBlue, AccuClear and AccuGreen Technology Highlights to choose the kit that best fits your application.

Category: DNA Quantitation Kits

← FAQs

The AccuClear™ and AccuBlue™ DNA Quantitation kits are designed for use with fluorescence 96-well plate readers. AccuClear™, AccuBlue™ NextGen and AccuBlue™ High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). AccuBlue™ Broad Range requires an instrument equipped to read blue fluorescence emission (Ex/Em 350/460 nm).

These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor™-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kit, which is designed for use on that instrument.

The AccuGreen™ High Sensitivity DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA High Sensitivity assay, and is a direct replacement for the Qubit® dsDNA HS Assay kit.

Category: DNA Quantitation Kits

← FAQs

For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue™ NextGen dsDNA Quantitation Kit (detection range 2.5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format.

Also see our AccuClear™ Ultra High Sensitivity dsDNA Quantation Kit, which is a great all-purpose choice: it has high sensitivity with a broad linear detection range (30 pg-250 ng dsDNA).

If you own a Qubit® fluorometer, we recommend our AccuGreen™ High Sensitivity dsDNA Quantitation kit, which is designed for use with the Qubit® fluorometer. It is a direct replacement for the Qubit® dsDNA HS Assay kit.

Note that the lower limits of kit sensitivity may depend on the detection instrument used.

Category: DNA Quantitation Kits

← FAQs

← FAQs

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.