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AccuBlue® High Sensitivity dsDNA Quantitation Kit with DNA Standards

AccuBlue® High Sensitivity is a non-mutagenic fluorescent dsDNA quantitation assay for fluorescence microplate reader. The kit offers sensitive and selective detection of purified dsDNA samples with excellent linearity.

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Product Description

AccuBlue® High Sensitivity dsDNA Quantitation Assay is a safer fluorescent dsDNA quantitation assay for microplate reader. The assay uses non-toxic, non-mutagenic EvaGreen® dye (download the EvaGreen Safety Report for more information). Featuring our unique AccuBlue® High Sensitivity Enhancer, the assay provides highly sensitive and selective detection of purified dsDNA samples with excellent linearity. The kits include calf thymus dsDNA standards, and provide sufficient reagents to perform 200 assays (trial size) or 1000 assays (regular size) based on a 96-well microplate format. AccuBlue® High Sensitivity solution components also are available without standards (catalog no. 31008), for customers who wish to use their own DNA standards.

The assay is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers. Note that while the assay is spectrally compatible with Qubit® fluorometers from Thermo Fisher, it may not be compatible with the high sensitivity dsDNA program on all Qubit® models (see our DNA Quantitation FAQs for more information). For the Qubit® we recommend our AccuGreen™ High Sensitivity Kit (catalog no. 31066), which was specially formulated for that instrument.

Features

  • Linear detection range from 0.2 – 100 ng
  • Non-toxic, non-mutagenic EvaGreen® dye – safer than PicoGreen® or Quant-iT® kits
  • AccuBlue® High Sensitivity Enhancer provides high sensitivity and excellent linearity

Kit Components

200 Assays (Trial Size)

  • AccuBlue® High Sensitivity dsDNA Quantitation Solution, 1X
  • AccuBlue® High Sensitivity Enhancer, 100X
  • AccuBlue® dsDNA Standard, 10 ng/uL

1000 Assays

  • AccuBlue® High Sensitivity dsDNA Quantitation Solution, 1X
  • AccuBlue® High Sensitivity Enhancer, 100X
  • AccuBlue® dsDNA Standards, set of 8

 

DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)
Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.

References

1. Conservation Genet Resour(2012), doi: 10.1007/s12686-011-9450-3

2. Parasites & Vectors (2012), doi:10.1186/1756-3305-5-59

3. Journal of Chromatography A (2010), doi:10.1016/j.chroma.2013.01.065

4. PLoS ONE (2013), doi:10.1371/journal.pone.0054743

5. Journal of Chromatography A (2013), http://dx.doi.org/doi:10.1016/j.chroma.2013.11.039

6. Journal of Biomechanics (2013), http://dx.doi.org/10.1016/j.jbiomech.2013.10.036

7. Peer J (2013), http://dx.doi.org/10.7717/peerj.203

8. Journal of Biomechanics (2014), http://dx.doi.org/10.1016/j.jbiomech.2013.10.036

9. Analytical Biochemistry (2014),  http://dx.doi.org/10.1016/j.ab.2014.03.005

10. J. Bacteriol. (2014), doi:10.1128/JB.01493-14

11. The International Journal of Biochemistry & Cell Biology (2014), doi: 10.1016/j.biocel.2014.04.026

12. BMC Oral Health (2014), doi: 10.1186/1472-6831-14-157

13. Applications in Plant Sciences (2015), http://dx.doi.org/10.3732/apps.1400077

14. Journal of Biotechnology (2015), http://dx.doi.org/10.1016/j.jbiotec.2015.04.006

15. Conserv Genet (2015), doi: 10.1007/s10592-015-0784-3

16. Am J Physiol Regul Integr Comp Physiol (2015), doi:10.1152/ajpregu.00421.2015

17. Marine Genomics (2015), doi: 10.1016/j.margen.2015.10.010

18. Drury et al. BMC Genomics (2016), doi: 10.1186/s12864-016-2583-8

19. Mitochondrial DNA Part B Resources (2016), http://dx.doi.org/10.1080/23802359.2016.1149787

20. Antonie van Leeuwenhoek (2017), doi: 10.1007/s10482-017-0925-4

21. Mitochondrial DNA Part B (2018), doi: 10.1080/23802359.2018.1473737

 

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Support & Faq

DNA Quantitation Kits

AccuGreen™ kits are designed for use with the Qubit® fluorometer. All of the other kits are designed for use with 96-well fluorescence plate readers. Our quantitation kits vary in the concentration ranges of dsDNA that they are able to detect. Some of the kits also have different fluorescence excitation/emission. Visit the DNA Quantitation Kit Technology Page or consult the table below to make your selection.

DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)
Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

Category: DNA Quantitation Kits

← FAQs

The AccuClear® and AccuBlue® DNA Quantitation kits are designed for use with fluorescence 96-well plate readers. AccuClear®, AccuBlue® NextGen and AccuBlue® High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). AccuBlue® Broad Range requires an instrument equipped to read blue fluorescence emission (Ex/Em 350/460 nm).

These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor™-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kits, which are designed for use on that instrument.

The AccuGreen™ High Sensitivity DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA High Sensitivity assay, and is a direct replacement for the Qubit® dsDNA HS Assay kit.

The AccuGreen™ Broad Range DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA Broad Range assay, and is a replacement for the Qubit® dsDNA BR Assay kit.

Category: DNA Quantitation Kits

← FAQs

For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2.5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format.

Also see our AccuClear® Ultra High Sensitivity dsDNA Quantation Kit, which is a great all-purpose choice: it has high sensitivity with a broad linear detection range (30 pg-250 ng dsDNA).

If you own a Qubit® fluorometer, we recommend our AccuGreen™ kits, which were designed for use with the Qubit® fluorometer.  AccuGreen™ High Sensitivity dsDNA Quantitation kit is a direct replacement for the Qubit® dsDNA HS Assay kit. AccuGreen™ Broad Range dsDNA Quantitation Kit is a replacement for the Qubit® dsDNA BR Assay kit.

Note that the lower limits of kit sensitivity may depend on the detection instrument used.

Category: DNA Quantitation Kits

← FAQs

← FAQs

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Please request expedited shipping in the Order Notes section if desired. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.