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AccuBlue® High Sensitivity dsDNA Quantitation Kit with DNA Standards

A fluorescent dsDNA quantitation kit with a linear range of 0.2-100 ng DNA. Designed for use in a fluorescence microplate reader.

Product Attributes

Excitation/Emission

500/530 nm (with DNA)

Storage Conditions

Store at 2 to 8 °C

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Trial size (200 assays)
1,000 assays
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Product Description

The AccuBlue® High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with a linear detection range from 0.2 – 100 ng. The AccuBlue® High Sensitivity assay is also available without any DNA standards (31008), for those that wish to use their own standard.

Features

  • Highly specific for dsDNA
  • Uses the non-toxic, non-mutagenic EvaGreen® dye for safer handling and easy disposal
  • Linear detection range from 0.2-100 ng
  • Designed for fluorescence microplate readers
  • Green fluorescence emission

Kit Components

  • AccuBlue® High Sensitivity dsDNA Quantitation Solution, 1X
  • AccuBlue® High Sensitivity Enhancer, 100X
  • AccuBlue® dsDNA Standards, set of 8 (in 1000 assay kit)
  • AccuBlue® dsDNA Standard, 10 ng/uL (in trial size kit)

The AccuBlue® High Sensitivity dsDNA Quantitation Assay uses non-toxic, non-mutagenic EvaGreen® dye (download the EvaGreen Safety Report for more information), making it safer than PicoGreen® or Quant-iT® kits.

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)
Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and PicoGreen are registered trademarks of Thermo Fisher Scientific.

References

1. Conservation Genet Resour (2011), 3, 753-755 doi: 10.1007/s12686-011-9450-3
2. Parasites & Vectors (2012), 5:59 doi:10.1186/1756-3305-5-59
3. PLoS ONE (2013), 8(1) doi:10.1371/journal.pone.0054743
4. Journal of Chromatography A (2013), doi:10.1016/j.chroma.2013.11.039
5. Peer J (2013), Nov 19;1:e203 doi: 10.7717/peerj.203
6. J Biomech (2014), 47(2), 518-525 doi:10.1016/j.jbiomech.2013.10.036
7. Analytical Biochemistry (2014),  http://dx.doi.org/10.1016/j.ab.2014.03.005
8. J. Bacteriol. (2014), Jul;196(13)2355-66 doi:10.1128/JB.01493-14
9. Applications in Plant Sciences (2015), doi: 10.3732/apps.1400077
10. Conserv Genet (2016), 17, 337-353 doi: 10.1007/s10592-015-0784-3
11. Invertebrate Biology (2015), 134(3) pp 252-259 doi: 10.1111/ivb.12091
12. Clin Oral Invest (2016) Jan;21(1) 447-452 doi: 10.1007/s00784-016-1811-6
13. Pharm res (2018), 35, 188 doi 10.1007/s11095-018-2460-z

 

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