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AccuGreen™ Broad Range dsDNA Quantitation Kit

DNA quantitation kit designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL.

Product Attributes

Excitation/Emission

500/530 nm (with DNA)

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500 assays
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Product Description

The AccuGreen™ Broad Range dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ kit is specific for dsDNA, and can quantify DNA samples in the range of 0.2-100 ng/uL. AccuGreen™ Broad Range Quantitation Solution also is available separately (catalog no. 31070) for customers who wish to use their own DNA standards.

For a high sensitivity assay to quantify DNA in the range of 0.01-10 ng/uL on the Qubit® fluorometer, see the AccuGreen™ High Sensitivity dsDNA Quantitation Kit (catalog no. 31066).

For broad range DNA quantitation using a fluorescent plate reader, we recommend using our AccuBlue® Broad Range dsDNA Quantitation Kit (catalog no. 31007). See the table below for details on our full line of dsDNA quantitation kits.

Features

  • Optimized for use with the Qubit® Fluorometer
  • Uses the pre-programmed Qubit® dsDNA Broad Range assay setting
  • Linear range in assay: 2-1000 ng dsDNA
  • Green fluorescence (Ex/Em 500/530 nm)

Components

  • AccuGreen™ Broad Range DNA Quantitation Solution, 1X
  • AccuGreen™ Standard 1 (0 ng/uL)
  • AccuGreen™ BR Standard 2 (100 ng/uL)
A genomic DNA sample was quantified using both the Qubit® BR kit and the AccuGreen™ BR kit. A nearly identical result was obtained with each kit.

All DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)
Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.

 

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