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DNA Quantitation Kits
AccuBlue® Broad Range dsDNA Standards, Set of Nine

AccuBlue® Broad Range dsDNA Standards, Set of Nine

Stand-alone set of the nine double-stranded DNA standards provided with the AccuBlue® Broad Range dsDNA Quantitation Kit (31007). Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL, 0.5 mL each.

DNA Quantitation

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PI-31006C, 31007C Download ( pdf )
SDS-31006C-31007C Download ( pdf )
DNA Quantitation Flyer Download ( pdf )
Molecular Biology Brochure Download ( pdf )

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TECHNOLOGY HIGHLIGHT
DNA Quantitation Kits Precise quantitation of purified dsDNA samples across a wide range of concentrations.
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Product Description

Stand-alone set of the nine double-stranded DNA standards provided with the AccuBlue® Broad Range dsDNA Quantitation Kit (31007). Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 2, 6.25, 12.5, 25, 50, 100, 150, and 200 ng/uL, 0.5 mL each.

Other DNA Quantitation Kits:

DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)		Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

References

1. PLoS ONE (2013), doi:10.1371/journal.pone.0080613

2. J Thromb Thrombolytic (2014), doi: 10.1007/s11239-014-1137-7

3. Molecular Ecology (2015), doi: 10.1111/mec.13167

 

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Support & Faq

DNA Quantitation Kits
What are the differences between the DNA quantitation kits? Which should I choose?

AccuGreen™ kits are designed for use with the Qubit® fluorometer. All of the other kits are designed for use with 96-well fluorescence plate readers. Our quantitation kits vary in the concentration ranges of dsDNA that they are able to detect. Some of the kits also have different fluorescence excitation/emission. Visit the DNA Quantitation Kit Technology Page or consult the table below to make your selection.

DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)		Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

Category: DNA Quantitation Kits

← FAQs
What instruments can I use with your DNA quantitation kits? Do they work on Qubit®?

The AccuClear® and AccuBlue® DNA Quantitation kits are designed for use with fluorescence 96-well plate readers. AccuClear®, AccuBlue® NextGen and AccuBlue® High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). AccuBlue® Broad Range requires an instrument equipped to read blue fluorescence emission (Ex/Em 350/460 nm).

These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor™-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kits, which are designed for use on that instrument.

The AccuGreen™ High Sensitivity DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA High Sensitivity assay, and is a direct replacement for the Qubit® dsDNA HS Assay kit.

The AccuGreen™ Broad Range DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA Broad Range assay, and is a replacement for the Qubit® dsDNA BR Assay kit.

Category: DNA Quantitation Kits

← FAQs
Which kits are the best alternatives for ThermoFishers Quant-iT™ and Qubit® kits?

For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2.5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format.

Also see our AccuClear® Ultra High Sensitivity dsDNA Quantation Kit, which is a great all-purpose choice: it has high sensitivity with a broad linear detection range (30 pg-250 ng dsDNA).

If you own a Qubit® fluorometer, we recommend our AccuGreen™ kits, which were designed for use with the Qubit® fluorometer.  AccuGreen™ High Sensitivity dsDNA Quantitation kit is a direct replacement for the Qubit® dsDNA HS Assay kit. AccuGreen™ Broad Range dsDNA Quantitation Kit is a replacement for the Qubit® dsDNA BR Assay kit.

Note that the lower limits of kit sensitivity may depend on the detection instrument used.

Category: DNA Quantitation Kits

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Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Please request expedited shipping in the Order Notes section if desired. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.