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AccuBlue® High Sensitivity dsDNA Quantitation Solution

The AccuBlue®  High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The solution is intended for use on a fluorecent plate reader in a 96-well microplate format.

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Product Description

The AccuBlue®  High Sensitivity dsDNA Quantitation Solution offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The Solution provides sufficient reagents to perform 1000 assays based on a 96-well microplate format. This item is a standalone fluorescent DNA quantitation solution, and is intended for customers who wish to use their own DNA standards. We also sell the complete AccuBlue® High Sensitivity Quantitation Kit that includes a set of standards (catalog no. 31006).

The assay is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers. Note that while the assay is spectrally compatible with Qubit® fluorometers from Thermo Fisher, it may not be compatible with the high sensitivity dsDNA program on all Qubit® models (see our DNA Quantitation FAQs for more information). For the Qubit® fluorometer we recommend our AccuGreen™ High Sensitivity Kit (catalog no. 31066), which was specially formulated for that instrument.

Features

  • linear detection range from 0.2 – 100 ng
  • Safer than PicoGreen® or Quant-iT® dsDNA detection kits: the high sensitivity reagent is impermeable to cell membranes

Components

  • AccuBlue® High Sensitivity dsDNA Quantitation Solution, 1X
  • AccuBlue® High Sensitivity Enhancer, 100X

All DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)
Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.

References

1. BMC Oral Health (2014), doi: 10.1186/1472-6831-14-157
2. Mitochondrial DNA Part B (2018), doi: 10.1080/23802359.2018.1473737

 

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