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Nucleic Acid Quantitation & Extraction
DNA Quantitation Kits
AccuBlue® High Sensitivity dsDNA Standards, Set of Eight

AccuBlue® High Sensitivity dsDNA Standards, Set of Eight

Stand-alone set of the eight double-stranded DNA standards provided with the AccuBlue® High Sensitivity dsDNA Quantitation Kit (31006).

 

AccuBlue® High Sensitivity dsDNA Standards, Set of Eight

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Store at 2 to 8 °C

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DNA & RNA Quantitation Kits
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FAQs

DNA & RNA Quantitation Kits
The signal of the AccuBlue® NextGen samples is very low

Note that the optimal excitation and emission maxima of AccuBlue® NextGen are 468/507 nm which is different than the other AccuBlue® dyes. The gain settings on your fluorescence plate reader may need to be adjusted for optimal signal to background.


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My product was accidentally left out at room temperature or exposed to light. Is it ruined?

Most of our products are stable at room temperature for many days, so in all likelihood the product will still work just fine. To be on the safe side, we recommend performing a small scale positive control experiment to confirm that the product still works for your application before processing a large number of samples or precious samples.

One exception that we are aware of is GelGreen™, which is more sensitive to light exposure than most of our other fluorescent dyes. If GelGreen™ is exposed to ambient light for a prolonged period of time (days to weeks), its color will change from dark orange to brick red. If this occurs, the GelGreen will no longer work for gel staining.

 


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What are the differences between the DNA quantitation kits? Which should I choose?

AccuGreen™ kits are designed for use with the Qubit® fluorometer. All of the other kits are designed for use with 96-well fluorescence plate readers. Our quantitation kits vary in the concentration ranges of dsDNA that they are able to detect. Some of the kits also have different fluorescence excitation/emission. Visit the DNA Quantitation Kit Technology Page or consult the table below to make your selection.

All DNA & RNA Quantitation Kits

Kit DNA
or RNA		Detection range
(in assay)*		Dye Ex/Em (nm)Suggested instrumentFeatures
AccuGreen™
High Sensitivity DNA		DNA0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range DNADNA2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen DNADNA1-3000 pg**468/507Fluorescence microplate readerMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
AccuClear® Ultra High Sensitivity DNADNA0.03-250 ng468/507Fluorescence microplate readerVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity DNADNA0.2-100 ng485/530Fluorescence microplate readerNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range DNADNA2-2000 ng350/460Fluorescence microplate readerBroad linear range with blue fluorescence
AccuBlue® Broad Range RNARNA5-1000 ng650/670Fluorescence microplate reader
or Qubit® fluorometer		The widest linear range of available RNA quantitation kits
Exceptional accuracy, sensitivity, and high RNA selectivity
* Standard assay volume is 200 uL
** AccuBlue® NextGen limit of detection is in the range of 1 pg to 5 pg depending on instrument sensitivity


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Product Description

Stand-alone set of the eight double-stranded DNA standards provided with the AccuBlue® High Sensitivity dsDNA Quantitation Kit (31006).

  • Calf thymus dsDNA in 1X Tris-EDTA
  • Set of eight standards at 0, 0.5, 1, 2, 4, 6, 8 and 10 ng/uL
  • 0.5 mL each

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA & RNA Quantitation Kits

Kit DNA
or RNA		Detection range
(in assay)*		Dye Ex/Em (nm)Suggested instrumentFeatures
AccuGreen™
High Sensitivity DNA		DNA0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range DNADNA2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen DNADNA1-3000 pg**468/507Fluorescence microplate readerMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
AccuClear® Ultra High Sensitivity DNADNA0.03-250 ng468/507Fluorescence microplate readerVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity DNADNA0.2-100 ng485/530Fluorescence microplate readerNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range DNADNA2-2000 ng350/460Fluorescence microplate readerBroad linear range with blue fluorescence
AccuBlue® Broad Range RNARNA5-1000 ng650/670Fluorescence microplate reader
or Qubit® fluorometer		The widest linear range of available RNA quantitation kits
Exceptional accuracy, sensitivity, and high RNA selectivity
* Standard assay volume is 200 uL
** AccuBlue® NextGen limit of detection is in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents.

References

1. BMC Oral Health (2014), doi: 10.1186/1472-6831-14-157
2. Infection, Genetics and Evolution(2017), http://dx.doi.org/10.1016/j.meegid.2017.02.006

 

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