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DNA Quantitation Kits
AccuBlue® High Sensitivity dsDNA Standards, Set of Eight

AccuBlue® High Sensitivity dsDNA Standards, Set of Eight

Stand-alone set of the eight double-stranded DNA standards provided with the AccuBlue® High Sensitivity dsDNA Quantitation Kit (31006). Calf thymus dsDNA at 0, 0.5, 1, 2, 4, 6, 8 and 10 ng/uL, 0.5 mL each.  

AccuBlue® High Sensitivity dsDNA Standards, Set of Eight

Protocols & Supporting Documents

PI-31006C, 31007C Download ( pdf )
SDS-31006C-31007C Download ( pdf )
DNA Quantitation Flyer Download ( pdf )
Molecular Biology Brochure Download ( pdf )
Learn more about
DNA Quantitation Kits Precise quantitation of purified dsDNA samples across a wide range of concentrations.

FAQs

DNA Quantitation Kits
What are the differences between the DNA quantitation kits? Which should I choose?

AccuGreen™ kits are designed for use with the Qubit® fluorometer. All of the other kits are designed for use with 96-well fluorescence plate readers. Our quantitation kits vary in the concentration ranges of dsDNA that they are able to detect. Some of the kits also have different fluorescence excitation/emission. Visit the DNA Quantitation Kit Technology Page or consult the table below to make your selection.

DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)		Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

Category: DNA Quantitation Kits

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What instruments can I use with your DNA quantitation kits? Do they work on Qubit®?

The AccuClear® and AccuBlue® DNA Quantitation kits are designed for use with fluorescence 96-well plate readers. AccuClear®, AccuBlue® NextGen and AccuBlue® High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). AccuBlue® Broad Range requires an instrument equipped to read blue fluorescence emission (Ex/Em 350/460 nm).

These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor™-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kits, which are designed for use on that instrument.

The AccuGreen™ High Sensitivity DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA High Sensitivity assay, and is a direct replacement for the Qubit® dsDNA HS Assay kit.

The AccuGreen™ Broad Range DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA Broad Range assay, and is a replacement for the Qubit® dsDNA BR Assay kit.

Category: DNA Quantitation Kits

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Which kits are the best alternatives for ThermoFishers Quant-iT™ and Qubit® kits?

For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue® NextGen dsDNA Quantitation Kit (detection range 2.5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format.

Also see our AccuClear® Ultra High Sensitivity dsDNA Quantation Kit, which is a great all-purpose choice: it has high sensitivity with a broad linear detection range (30 pg-250 ng dsDNA).

If you own a Qubit® fluorometer, we recommend our AccuGreen™ kits, which were designed for use with the Qubit® fluorometer.  AccuGreen™ High Sensitivity dsDNA Quantitation kit is a direct replacement for the Qubit® dsDNA HS Assay kit. AccuGreen™ Broad Range dsDNA Quantitation Kit is a replacement for the Qubit® dsDNA BR Assay kit.

Note that the lower limits of kit sensitivity may depend on the detection instrument used.

Category: DNA Quantitation Kits

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Product Description

Stand-alone set of the eight double-stranded DNA standards provided with the AccuBlue® High Sensitivity dsDNA Quantitation Kit (31006). Calf thymus dsDNA in 10 mM Tris pH 7.5, 1 mM EDTA, 2 mM sodium azide at 0, 0.5, 1, 2, 4, 6, 8 and 10 ng/uL, 0.5 mL each.

Other DNA Quantitation Kits:

DNA Quantitation Kits

dsDNA Quantitation KitLinear detection range
(dsDNA in assay)		Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity0.1-100 ng502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range2-1000 ng500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen1 pg-3 ng*468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity30 pg-250 ng468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity0.2-100 ng485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range2-2000 ng350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence

References

1. BMC Oral Health (2014), doi: 10.1186/1472-6831-14-157

2. Infection, Genetics and Evolution(2017), http://dx.doi.org/10.1016/j.meegid.2017.02.006

 

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