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AccuBlue® High Sensitivity dsDNA Standards, Set of Eight

Stand-alone set of the eight double-stranded DNA standards provided with the AccuBlue® High Sensitivity dsDNA Quantitation Kit (31006).

 

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Store at 2 to 8 °C

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Product Description

Stand-alone set of the eight double-stranded DNA standards provided with the AccuBlue® High Sensitivity dsDNA Quantitation Kit (31006).

  • Calf thymus dsDNA in 1X Tris-EDTA
  • Set of eight standards at 0, 0.5, 1, 2, 4, 6, 8 and 10 ng/uL
  • 0.5 mL each

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA & RNA Quantitation Kits

Kit NameDNA or RNADye Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity DNADNA502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range DNADNA500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen DNADNA468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity DNADNA468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity DNADNA485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range DNADNA350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence
AccuBlue® Broad Range RNARNA650/670Fluorescent Plate Reader or Qubit® fluorometerThe widest linear range of available RNA quantitation kits, with exceptional accuracy, sensitivity, and high RNA selectivity
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents.

References

1. BMC Oral Health (2014), doi: 10.1186/1472-6831-14-157
2. Infection, Genetics and Evolution(2017), http://dx.doi.org/10.1016/j.meegid.2017.02.006

 

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