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AccuBlue NextGen dsDNA Quantitation Solution

AccuBlue NextGen dsDNA Quantitation Solution

The AccuBlue™ NextGen dsDNA Quantitation Solution provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue™ NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA.

AccuGreen High Sensitivity dsDNA Quantitation Solution

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Catalog #

31061, - 31061-TView allHide
Size

Trial Size, 1,000 assays
TECHNOLOGY HIGHLIGHT
AccuBlue™ AccuClear™ & AccuGreen™ dsDNA Quantitation Kits Precise quantitation of purified dsDNA samples across a wide range of concentrations.
Support & FAQ
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Product Description

The AccuBlue™ NextGen dsDNA Quantitation Solution provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue™ NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA. With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue™ NextGen outperforms rivals such as PicoGreen® in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue™ NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.

The AccuBlue™ NextGen dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuBlue™ NextGen Dye make it especially well-suited for use with instruments with blue LED excitation sources. Because the AccuBlue™ NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see Table 1), assay volume, and pipet accuracy.

The AccuBlue™ NextGen dsDNA Quantitation Solution contains AccuBlue™ NextGen Dye, Enhancer, and assay buffer for 1000 96-well assays; the trial size kit contains enough reagents for 200 96-well assays. The Quantitation Solution does not include a DNA standard; see the AccuBlue™ NextGen dsDNA Quantitation Kit, which includes a calf thymus dsDNA standard.

Kit components

  • AccuBlue™ NextGen Dye, 400X
  • AccuBlue™ NextGen Enhancer, 100X
  • AccuBlue™ NextGen Buffer, 1X

Features

  • Linear range: 1-3000 pg dsDNA
  • Green fluorescence (Ex/Em 460/507 nm)
  • Unrivaled sensitivity
  • Green fluorescent dye is a perfect match for blue LED excitation sources
  • Green fluorescence detection compatible with fluorescence microplate readers, handheld fluorometers, and  NanoDrop® fluorospectrophotometers.
readerassay format (assay volume)lowest DNA detected
BMG CLARIOStar96-well (200 uL)1 pg
BMG CLARIOStar384-well (50 uL)0.6 pg
Biotek Synergy H1m96-well (200 uL)5 pg
SpectraMax Gemini XS96-well (200 uL)5 pg
SpectraMax i3384-well (50 uL)1.25 pg

Other DNA Quantitation Kits:

dsDNA Quantitation KitLinear detection range (dsDNA in assay)Ex/Em (nm)Features
AccuBlue™ NextGen1 pg*-3 ng468/507Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity.
AccuClear™ Ultra High Sensitivity30 pg-250 ng468/507This kit offers great sensitivity and the widest linear range.

AccuBlue™ High Sensitivity0.2-100 ng485/530Quantitation of dsDNA from 0.2-100 ng, with a safer, membrane impermeable DNA dye.
AccuGreen™ High Sensitivity0.1-100 ng502/523Quantitation of DNA using the Qubit® fluorometer from Thermo Fisher.
AccuBlue™ Broad Range2-2000 ng350/460For quantitation of high concentration DNA in a broad linear range with blue fluorescence. Assay can be extended to 4000 ng with minor loss of linearity.

AccuBlue NextGen technology is covered by U.S. patents.

References

n/a

 

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    AccuBlue NextGen dsDNA Quantitation Kit

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    RNase-Free Calf Thymus DNA, 1 mg/mL

    This double-stranded Calf Thymus DNA is free of RNase activity, and is useful in applications where RNase contamination needs to be avoided. It has been prepared using Biotium's proprietary method to irreversibly inactivate RNase activity, and tested to confirm that there is no RNAse activity remaining.
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    AccuBlue High Sensitivity dsDNA Quantitation Kit with DNA Standards

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Support & Faq

DNA Quantitation Kits
What are the differences between the DNA quantitation kits? Which should I choose?

AccuGreen™ kits are designed for use with the Qubit® fluorometer. All of the other kits are designed for use with 96-well fluorescence plate readers. Our quantitation kits vary in the concentration ranges of dsDNA that they are able to detect. Some of the kits also have different fluorescence excitation/emission. Please see the AccuBlue, AccuClear and AccuGreen Technology Highlights to choose the kit that best fits your application.

Category: DNA Quantitation Kits

← FAQs
What instruments can I use with your DNA quantitation kits? Do they work on Qubit®?

The AccuClear™ and AccuBlue™ DNA Quantitation kits are designed for use with fluorescence 96-well plate readers. AccuClear™, AccuBlue™ NextGen and AccuBlue™ High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). AccuBlue™ Broad Range requires an instrument equipped to read blue fluorescence emission (Ex/Em 350/460 nm).

These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor™-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kit, which is designed for use on that instrument.

The AccuGreen™ High Sensitivity DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA High Sensitivity assay, and is a direct replacement for the Qubit® dsDNA HS Assay kit.

Category: DNA Quantitation Kits

← FAQs
Which kits are the best alternatives for ThermoFishers Quant-iT™ and Qubit® kits?

For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue™ NextGen dsDNA Quantitation Kit (detection range 2.5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format.

Also see our AccuClear™ Ultra High Sensitivity dsDNA Quantation Kit, which is a great all-purpose choice: it has high sensitivity with a broad linear detection range (30 pg-250 ng dsDNA).

If you own a Qubit® fluorometer, we recommend our AccuGreen™ High Sensitivity dsDNA Quantitation kit, which is designed for use with the Qubit® fluorometer. It is a direct replacement for the Qubit® dsDNA HS Assay kit.

Note that the lower limits of kit sensitivity may depend on the detection instrument used.

Category: DNA Quantitation Kits

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