The AccuBlue® NextGen dsDNA Quantitation Solution provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA. With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue® NextGen outperforms rivals such as PicoGreen® in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue® NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.
The AccuBlue® NextGen dsDNA Quantitation Solution does not come with any DNA standards. It is intended customers who wish to use their own standards. We also offer the complete AccuBlue® NextGen dsDNA Quantitation Kit (catalog no. 31060) that comes with a 10 ng/uL dsDNA standard, with instructions for preparing a set of standards by serial dilution.
The AccuBlue® NextGen dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuBlue® NextGen Dye make it especially well-suited for use with instruments with blue LED excitation sources. Because the AccuBlue® NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see table below), assay volume, and pipet accuracy.
For DNA quantitation using the Qubit® fluorometer we recommend our AccuGreen™ High Sensitivity dsDNA Quantitation Kit (catalog no. 31066) and AccuGreen™ Broad Range dsDNA Quantitation Kit (catalog no. 31069), which were formulated specifically for that instrument.
- Linear range: 1-3000 pg dsDNA
- Unrivaled sensitivity
- Green fluorescent dye is a perfect match for blue LED excitation sources
- AccuBlue® NextGen Dye, 400X
- AccuBlue® NextGen Enhancer, 100X
- AccuBlue® NextGen Buffer, 1X
Plate Reader Detection Limits
|Plate reader||Assay format (assay volume)||Lowest DNA detected
|BMG CLARIOStar||96-well (200 uL)||1 pg
|BMG CLARIOStar||384-well (50 uL)||0.6 pg
|Biotek Synergy H1m||96-well (200 uL)||5 pg
|SpectraMax Gemini XS||96-well (200 uL)||5 pg
|SpectraMax i3||384-well (50 uL)||1.25 pg
DNA Quantitation Kits
|dsDNA Quantitation Kit||Linear detection range|
(dsDNA in assay)
|Ex/Em (nm)||Suggested Instrument||Features
| AccuGreen High Sensitivity||0.1-100 ng||502/523||Qubit® fluorometer||Compare to the Qubit® dsDNA HS assay from Thermo Fisher
| AccuGreen Broad Range||2-1000 ng||500/530||Qubit® fluorometer||Compare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
| AccuBlue® NextGen||1 pg-3 ng*||468/507||Fluorescent Plate Reader||Most sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
| AccuClear® Ultra High Sensitivity||30 pg-250 ng||468/507||Fluorescent Plate Reader||Versatile kit with high sensitivity and wide linear range
| AccuBlue® High Sensitivity||0.2-100 ng||485/530||Fluorescent Plate Reader||Non-toxic & non-mutagentic DNA quantitation dye
| AccuBlue® Broad Range||2-2000 ng||350/460||Fluorescent Plate Reader||Broad linear range with blue fluorescence
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.