AccuBlue® NextGen dsDNA Quantitation Solution
A fluorescent dsDNA quantitation solution with a linear range of 1-3,000 pg DNA, an unprecedented level of sensitivity in dsDNA detection. Designed for use in a fluorescence microplate reader.
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Product Description
The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA, with a linear range from ~1 – 3,000 pg (depending on instrument capability). This assay is designed for use with fluorescence microplate readers. This solution does not come with a DNA standard; see the AccuBlue® NextGen Kit (31060), which contains a calf thymus DNA standard.
Features
- Most sensitive assay on the market: outperforms rivals such as PicoGreen® in sensitivity and accuracy
- Linear range: 1-3000 pg dsDNA (depending on plate reader capability)
- Green fluorescent dye is a perfect match for blue LED excitation sources
- Working solution is stable up to 6 months at 4C for extra convenience
Kit Components
- AccuBlue® NextGen Dye, 400X
- AccuBlue® NextGen Enhancer, 100X
- DNA Quantitation Buffer, 20X*
*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.
With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue® NextGen outperforms rivals such as PicoGreen® in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue® NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.
Because the AccuBlue® NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see table below), assay volume, and pipet accuracy.
Plate Reader Detection Limits
| Plate reader | Assay format (assay volume) | Lowest DNA detected |
|---|---|---|
| BMG CLARIOStar | 96-well (200 uL) | 1 pg |
| BMG CLARIOStar | 384-well (50 uL) | 0.6 pg |
| Biotek Synergy H1m | 96-well (200 uL) | 5 pg |
| SpectraMax Gemini XS | 96-well (200 uL) | 5 pg |
| SpectraMax i3 | 384-well (50 uL) | 1.25 pg |
Fluorescence-Based dsDNA Quantitation
AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.
Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.
All DNA Quantitation Kits
| dsDNA Quantitation Kit | Linear detection range (dsDNA in assay) | Ex/Em (nm) | Suggested Instrument | Features |
|---|---|---|---|---|
| AccuGreen™ High Sensitivity | 0.1-100 ng | 502/523 | Qubit® fluorometer | Compare to the Qubit® dsDNA HS assay from Thermo Fisher |
| AccuGreen™ Broad Range | 2-1000 ng | 500/530 | Qubit® fluorometer | Compare to the Qubit® dsDNA BR assay from Thermo Fisher Non-toxic & non-mutagentic DNA quantitation dye |
| AccuBlue® NextGen | 1 pg-3 ng* | 468/507 | Fluorescent Plate Reader | Most sensitive assay available for quantitation of precious or dilute samples Optimal for sensitive applications such as NGS or digital PCR * Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity |
| AccuClear® Ultra High Sensitivity | 30 pg-250 ng | 468/507 | Fluorescent Plate Reader | Versatile kit with high sensitivity and wide linear range |
| AccuBlue® High Sensitivity | 0.2-100 ng | 485/530 | Fluorescent Plate Reader | Non-toxic & non-mutagentic DNA quantitation dye |
| AccuBlue® Broad Range | 2-2000 ng | 350/460 | Fluorescent Plate Reader | Broad linear range with blue fluorescence |
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and PicoGreen are registered trademarks of Thermo Fisher Scientific.
References
1. Analytical Biochemistry (2016) 15 Oct pp74-79 doi:10.1016/j.ab.2016.07.022
2. J Assist Reprod Genet (2018) 35, 1497-1501 doi:10.1007/s10815-018-1223-4