AccuBlue® NextGen dsDNA Quantitation Solution
The AccuBlue® NextGen dsDNA Quantitation Solution provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA.
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The AccuBlue® NextGen dsDNA Quantitation Solution provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA. With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue® NextGen outperforms rivals such as PicoGreen® in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue® NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.
The AccuBlue® NextGen dsDNA Quantitation Solution does not come with any DNA standards. It is intended customers who wish to use their own standards. We also offer the complete AccuBlue® NextGen dsDNA Quantitation Kit (catalog no. 31060) that comes with a 10 ng/uL dsDNA standard, with instructions for preparing a set of standards by serial dilution.
The AccuBlue® NextGen dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuBlue® NextGen Dye make it especially well-suited for use with instruments with blue LED excitation sources. Because the AccuBlue® NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see table below), assay volume, and pipet accuracy.
For DNA quantitation using the Qubit® fluorometer we recommend our AccuGreen™ High Sensitivity dsDNA Quantitation Kit (catalog no. 31066) and AccuGreen™ Broad Range dsDNA Quantitation Kit (catalog no. 31069), which were formulated specifically for that instrument.
Features
- Linear range: 1-3000 pg dsDNA
- Unrivaled sensitivity
- Green fluorescent dye is a perfect match for blue LED excitation sources
Components
- AccuBlue® NextGen Dye, 400X
- AccuBlue® NextGen Enhancer, 100X
- DNA Quantitation Buffer, 20X*
*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.
Plate Reader Detection Limits
| Plate reader | Assay format (assay volume) | Lowest DNA detected |
|---|---|---|
| BMG CLARIOStar | 96-well (200 uL) | 1 pg |
| BMG CLARIOStar | 384-well (50 uL) | 0.6 pg |
| Biotek Synergy H1m | 96-well (200 uL) | 5 pg |
| SpectraMax Gemini XS | 96-well (200 uL) | 5 pg |
| SpectraMax i3 | 384-well (50 uL) | 1.25 pg |
All DNA Quantitation Kits
| dsDNA Quantitation Kit | Linear detection range (dsDNA in assay) | Ex/Em (nm) | Suggested Instrument | Features |
|---|---|---|---|---|
| AccuGreen™ High Sensitivity | 0.1-100 ng | 502/523 | Qubit® fluorometer | Compare to the Qubit® dsDNA HS assay from Thermo Fisher |
| AccuGreen™ Broad Range | 2-1000 ng | 500/530 | Qubit® fluorometer | Compare to the Qubit® dsDNA BR assay from Thermo Fisher Non-toxic & non-mutagentic DNA quantitation dye |
| AccuBlue® NextGen | 1 pg-3 ng* | 468/507 | Fluorescent Plate Reader | Most sensitive assay available for quantitation of precious or dilute samples Optimal for sensitive applications such as NGS or digital PCR * Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity |
| AccuClear® Ultra High Sensitivity | 30 pg-250 ng | 468/507 | Fluorescent Plate Reader | Versatile kit with high sensitivity and wide linear range |
| AccuBlue® High Sensitivity | 0.2-100 ng | 485/530 | Fluorescent Plate Reader | Non-toxic & non-mutagentic DNA quantitation dye |
| AccuBlue® Broad Range | 2-2000 ng | 350/460 | Fluorescent Plate Reader | Broad linear range with blue fluorescence |
AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and Quant-iT are registered trademarks of Thermo Fisher Scientific.
References
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