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AccuBlue® NextGen dsDNA Quantitation Solution

A fluorescent dsDNA quantitation solution with a linear range of 1-3,000 pg DNA, an unprecedented level of sensitivity in dsDNA detection. Designed for use in a fluorescence microplate reader.

Product Attributes

Excitation/Emission

468/507 nm (with DNA)

Storage Conditions

Store at 2 to 8 °C

Note: Due to operating restrictions related to our COVID-19 response, primary antibody conjugates of R-PE, APC, PerCP, HRP, or AP are temporarily unavailable. All other antibody options are still available. We apologize for the inconvenience. Please see our easy-to-use Mix-n-Stain™ Antibody Labeling Kits for labeling antibodies with R-PE, APC, tandem dyes, and enzymes.
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Product Description

The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA, with a linear range from ~1 – 3,000 pg (depending on instrument capability). This assay is designed for use with fluorescence microplate readers. This solution does not come with a DNA standard; see the AccuBlue® NextGen Kit (31060), which contains a calf thymus DNA standard.

Features

  • Most sensitive assay on the market: outperforms rivals such as PicoGreen® in sensitivity and accuracy
  • Linear range: 1-3000 pg dsDNA (depending on plate reader capability)
  • Green fluorescent dye is a perfect match for blue LED excitation sources
  • Working solution is stable up to 6 months at 4C for extra convenience

Kit Components

  • AccuBlue® NextGen Dye, 400X
  • AccuBlue® NextGen Enhancer, 100X
  • DNA Quantitation Buffer, 20X*

*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.

With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue® NextGen outperforms rivals such as PicoGreen® in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue® NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.

Because the AccuBlue® NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see table below), assay volume, and pipet accuracy.

Plate Reader Detection Limits

Plate readerAssay format (assay volume)Lowest DNA detected
BMG CLARIOStar96-well (200 uL)1 pg
BMG CLARIOStar384-well (50 uL)0.6 pg
Biotek Synergy H1m96-well (200 uL)5 pg
SpectraMax Gemini XS96-well (200 uL)5 pg
SpectraMax i3384-well (50 uL)1.25 pg

Fluorescence-Based dsDNA Quantitation

AccuBlue®, AccuClear® and AccuGreen™ dsDNA quantitation assays allow precise quantitation of purified dsDNA samples across a wide range of concentrations and a variety of fluorescence detection instruments. Unlike absorbance-based nucleic acid quantitation, fluorescent DNA binding dyes are highly sensitive and selective for double-stranded DNA and provide a more accurate DNA concentration in the presence of contaminating RNA and other common contaminants including free nucleotides, protein, detergents and salts.

Biotium offers dsDNA quantitiation kits and solutions for different instruments and sample concentration ranges. See the table below and visit the DNA & RNA Quantitation Technology Page for details on our full line of dsDNA and RNA quantitation kits.

All DNA & RNA Quantitation Kits

Kit NameDNA or RNADye Ex/Em (nm)Suggested InstrumentFeatures
AccuGreen™ High Sensitivity DNADNA502/523Qubit® fluorometerCompare to the Qubit® dsDNA HS assay from Thermo Fisher
AccuGreen™ Broad Range DNADNA500/530Qubit® fluorometerCompare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
AccuBlue® NextGen DNADNA468/507Fluorescent Plate ReaderMost sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
AccuClear® Ultra High Sensitivity DNADNA468/507Fluorescent Plate ReaderVersatile kit with high sensitivity and wide linear range
AccuBlue® High Sensitivity DNADNA485/530Fluorescent Plate ReaderNon-toxic & non-mutagentic DNA quantitation dye
AccuBlue® Broad Range DNADNA350/460Fluorescent Plate ReaderBroad linear range with blue fluorescence
AccuBlue® Broad Range RNARNA650/670Fluorescent Plate Reader or Qubit® fluorometerThe widest linear range of available RNA quantitation kits, with exceptional accuracy, sensitivity, and high RNA selectivity

AccuBlue and AccuClear are registered trademarks of Biotium, Inc. AccuBlue, AccuClear and AccuGreen technologies are covered by granted U.S. and international patents. Qubit and PicoGreen are registered trademarks of Thermo Fisher Scientific.

References

1. Analytical Biochemistry (2016) 15 Oct pp74-79 doi:10.1016/j.ab.2016.07.022
2. J Assist Reprod Genet (2018) 35, 1497-1501 doi:10.1007/s10815-018-1223-4

 

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