The AccuBlue® NextGen dsDNA Quantitation Solution provides an unprecedented level of sensitivity in dsDNA detection. The AccuBlue® NextGen dsDNA Quantitation assay is the most sensitive and accurate DNA quantitation method for ultra low amounts of DNA. With a detection limit as low as 1 pg DNA in a 96-well plate reader assay (see Table 1), AccuBlue® NextGen outperforms rivals such as PicoGreen® in sensitivity and accuracy. Specially designed for scientists who need to quantify extremely dilute or precious DNA, AccuBlue® NextGen is ideal for use in preparing samples for applications such as Next Generation Sequencing (NGS) and digital PCR.
The AccuBlue® NextGen dsDNA quantitation assay is designed for use with fluorescence 96-well plate readers equipped with excitation and emission filters for detecting green fluorescence. The unique spectral properties of AccuBlue® NextGen Dye make it especially well-suited for use with instruments with blue LED excitation sources. Because the AccuBlue® NextGen assay is so sensitive, results will vary depending on many factors, such as microplate reader (see Table 1), assay volume, and pipet accuracy.
The AccuBlue® NextGen dsDNA Quantitation Solution contains AccuBlue® NextGen Dye, Enhancer, and assay buffer for 1000 96-well assays; the trial size kit contains enough reagents for 200 96-well assays. The Quantitation Solution does not include a DNA standard; see the AccuBlue® NextGen dsDNA Quantitation Kit, which includes a calf thymus dsDNA standard.
- AccuBlue® NextGen Dye, 400X
- AccuBlue® NextGen Enhancer, 100X
- AccuBlue® NextGen Buffer, 1X
- Linear range: 1-3000 pg dsDNA
- Green fluorescence (Ex/Em 460/507 nm)
- Unrivaled sensitivity
- Green fluorescent dye is a perfect match for blue LED excitation sources
- Green fluorescence detection compatible with fluorescence microplate readers, handheld fluorometers, and NanoDrop® fluorospectrophotometers.
|Plate reader||Assay format (assay volume)||Lowest DNA detected
|BMG CLARIOStar||96-well (200 uL)||1 pg
|BMG CLARIOStar||384-well (50 uL)||0.6 pg
|Biotek Synergy H1m||96-well (200 uL)||5 pg
|SpectraMax Gemini XS||96-well (200 uL)||5 pg
|SpectraMax i3||384-well (50 uL)||1.25 pg
Other DNA Quantitation Kits:
|dsDNA Quantitation Kit||Linear detection range|
(dsDNA in assay)
| AccuBlue® NextGen||1 pg-3 ng*||468/507||Most sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
| AccuClear® Ultra High Sensitivity||30 pg-250 ng||468/507||Versatile kit with high sensitivity and wide linear range
| AccuBlue® High Sensitivity||0.2-100 ng||485/530||Non-toxic & non-mutagentic membrane impermeable DNA dye
| AccuGreen High Sensitivity||0.1-100 ng||502/523||Quantitation of DNA using the Qubit® fluorometer from Thermo Fisher
| AccuBlue® Broad Range||2-2000 ng||350/460||Broad linear range with blue fluorescence
AccuBlue® NextGen technology is covered by U.S. patents. PicoGreen is a registered trademark of Thermo Fisher Scientific.