The AccuGreen™ High Sensitivity dsDNA Quantitation Solution is designed for use with handheld fluorometers such as the Qubit® fluorometers from Thermo Fisher. Unlike absorbance-based measurements, AccuGreen™ Dye is highly selective for double-stranded DNA over single-stranded DNA or RNA.
The linear range of the AccuGreen™ assay is 0.1 to 100 ng of DNA per tube. DNA samples with concentrations between 5 pg/uL and 100 ng/uL may be quantified using sample volumes between 1 and 20 uL (for example, 1 uL of 100 ng/uL is 100 ng total, and 20 uL of 5 pg/uL is 0.1 ng total, which will both fall within the linear range of the assay). If you use the most common sample volume of 10 uL, the starting sample concentration should be 10 pg/uL to 10 ng/uL.
The AccuGreen™ High Sensitivity dsDNA Quantitation Solution includes AccuGreen™ dye and assay buffer, but no DNA standards. It is intended for users who wish to use their own DNA standard. Biotium also offers the AccuGreen™ High Sensitivity dsDNA Quantitation Kit (catalog no. 31066), which includes dye, buffer, and a set of 0 and 10 ng/uL calf thymus dsDNA standards.
For a broad range assay to quantify DNA in the range of 0.2-100 ng/uL on the Qubit® fluorometer, see the AccuGreen™ Broad Range dsDNA Quantitation Kit (catalog no. 31069).
For high-sensitivity DNA quantitation using a fluorescent plate reader, we recommend using our AccuClear® or AccuBlue® NextGen dsDNA Quantitation Kits, which provide unprecedented sensitivity, accuracy and wide linear range. See the table below for details on our full line of dsDNA quantitation kits.
- Optimized for use with the Qubit® Fluorometer
- Uses the pre-programmed Qubit® dsDNA High Sensitivity assay setting
- Linear range of assay: 0.1-100 ng dsDNA
- Sample concentration range: 5 pg/uL to 100 ng/uL
- Green fluorescence (Ex/Em 502/523 nm)
- AccuGreen™ Dye, 200X
- DNA Quantitation Buffer, 20X Concentrate*
*Note: In order to go green and save on packaging and shipping, we have changed from a 1X to a 20X buffer.
All DNA Quantitation Kits
|dsDNA Quantitation Kit||Linear detection range|
(dsDNA in assay)
|Ex/Em (nm)||Suggested Instrument||Features
| AccuGreen High Sensitivity||0.1-100 ng||502/523||Qubit® fluorometer||Compare to the Qubit® dsDNA HS assay from Thermo Fisher
| AccuGreen Broad Range||2-1000 ng||500/530||Qubit® fluorometer||Compare to the Qubit® dsDNA BR assay from Thermo Fisher
Non-toxic & non-mutagentic DNA quantitation dye
| AccuBlue® NextGen||1 pg-3 ng*||468/507||Fluorescent Plate Reader||Most sensitive assay available for quantitation of precious or dilute samples
Optimal for sensitive applications such as NGS or digital PCR
* Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity
| AccuClear® Ultra High Sensitivity||30 pg-250 ng||468/507||Fluorescent Plate Reader||Versatile kit with high sensitivity and wide linear range
| AccuBlue® High Sensitivity||0.2-100 ng||485/530||Fluorescent Plate Reader||Non-toxic & non-mutagentic DNA quantitation dye
| AccuBlue® Broad Range||2-2000 ng||350/460||Fluorescent Plate Reader||Broad linear range with blue fluorescence
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