AccuGreen High Sensitivity dsDNA Quantitation Solution

DNA quantitation kits
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Catalog #: 31068, 31068-T
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Product Description

The AccuGreen™ High Sensitivity dsDNA Quantitation Solution is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ assay is specific for dsDNA, and can quantify DNA samples in the range of .01-10 ng/uL. For high-sensitivity DNA quantitation using a fluorescent plate reader, we recommend using our AccuClear™ or AccuBlue™ NextGen dsDNA Quantitation Kits, which provide unprecedented sensitivity, accuracy and wide linear range.

The AccuGreen™ High Sensitivity dsDNA Quantitation Solution includes AccuGreen™ dye and assay buffer. Biotium also offers the AccuGreen™ High Sensitivity dsDNA Quantitation Kit (catalog no. 31066), which includes dye, buffer, and a set of 0 and 10 ng/uL calf thymus dsDNA standards, for use in the Qubit®  pre-programmed dsDNA High Sensitivity assay.

Features

  • Optimized for use with the Qubit® Fluorometer
  • Uses the pre-programmed Qubit® dsDNA High Sensitivity assay setting
  • Linear range in assay: 0.1-100 ng dsDNA
  • Green fluorescence (Ex/Em 502/523 nm)

Components

  • AccuGreen™ Dye, 200X
  • AccuGreen™ Buffer, 1X

Kit sizes

  • The 500 assay kit (31068) contains enough reagents to perform 1,250 reactions, including standards (allowing the measurement of approximately 500 DNA samples)
  • The 100 assay trial kit (31068-T) contains enough reagents to perform 250 reactions, including standards (allowing the measurement of approximately 100 DNA samples)

Other DNA Quantitation Kits:

dsDNA Quantitation KitLinear detection range (dsDNA in assay)Ex/Em (nm)Features
AccuClear™ Ultra High Sensitivity30 pg-250 ng468/507This kit offers great sensitivity and the widest linear range.

AccuBlue™ Broad Range2-2000 ng350/460For quantitation of high concentration DNA in a broad linear range. Assay can be extended to 4000 ng with minor loss of linearity.

AccuBlue™ NextGen1 pg*-3 ng468/507Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity.
AccuBlue™ High Sensitivity0.2-100 ng485/530
AccuGreen™ High Sensitivity0.1-100 ng502/523Quantitation of DNA using the Qubit fluorometer from Thermo Fisher.

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Additional Information

Size

500 assays, 100 assays

MSDS (PDF): msds-31068

Protocol (PDF): PI-31068

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.

More information about product handling and shelf life