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AccuGreen High Sensitivity dsDNA Quantitation Solution

The AccuGreen™ High Sensitivity dsDNA Quantitation Solution is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ assay is specific for dsDNA, and can quantify DNA samples in the range of .01-10 ng/uL.

AccuGreen High Sensitivity dsDNA Quantitation Solution
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Catalog #

31068, - 31068-TView allHide

Size

100 assays, 500 assays

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Product Description

The AccuGreen™ High Sensitivity dsDNA Quantitation Solution is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ assay is specific for dsDNA, and can quantify DNA samples in the range of .01-10 ng/uL. For high-sensitivity DNA quantitation using a fluorescent plate reader, we recommend using our AccuClear™ or AccuBlue™ NextGen dsDNA Quantitation Kits, which provide unprecedented sensitivity, accuracy and wide linear range.

The AccuGreen™ High Sensitivity dsDNA Quantitation Solution includes AccuGreen™ dye and assay buffer. Biotium also offers the AccuGreen™ High Sensitivity dsDNA Quantitation Kit (catalog no. 31066), which includes dye, buffer, and a set of 0 and 10 ng/uL calf thymus dsDNA standards, for use in the Qubit®  pre-programmed dsDNA High Sensitivity assay.

Features

  • Optimized for use with the Qubit® Fluorometer
  • Uses the pre-programmed Qubit® dsDNA High Sensitivity assay setting
  • Linear range in assay: 0.1-100 ng dsDNA
  • Green fluorescence (Ex/Em 502/523 nm)

Components

  • AccuGreen™ Dye, 200X
  • AccuGreen™ Buffer, 1X

Kit sizes

  • The 500 assay kit (31068) contains enough reagents to perform 1,250 reactions, including standards (allowing the measurement of approximately 500 DNA samples)
  • The 100 assay trial kit (31068-T) contains enough reagents to perform 250 reactions, including standards (allowing the measurement of approximately 100 DNA samples)

Other DNA Quantitation Kits:

dsDNA Quantitation KitLinear detection range (dsDNA in assay)Ex/Em (nm)Features
AccuClear™ Ultra High Sensitivity30 pg-250 ng468/507This kit offers great sensitivity and the widest linear range.

AccuBlue™ Broad Range2-2000 ng350/460For quantitation of high concentration DNA in a broad linear range with blue fluorescence. Assay can be extended to 4000 ng with minor loss of linearity.

AccuBlue™ NextGen1 pg*-3 ng468/507Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity.
AccuBlue™ High Sensitivity0.2-100 ng485/530Quantitation of dsDNA from 0.2-100 ng, with a safer, membrane impermeable DNA dye.
AccuGreen™ High Sensitivity0.1-100 ng502/523Quantitation of DNA using the Qubit fluorometer from Thermo Fisher.

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Support & faq

DNA Quantitation Kits

AccuGreen™ kits are designed for use with the Qubit® fluorometer. All of the other kits are designed for use with 96-well fluorescence plate readers. Our quantitation kits vary in the concentration ranges of dsDNA that they are able to detect. Some of the kits also have different fluorescence excitation/emission. Please see the AccuBlue, AccuClear and AccuGreen Technology Highlights to choose the kit that best fits your application.

Category: DNA Quantitation Kits

← FAQs

The AccuClear™ and AccuBlue™ DNA Quantitation kits are designed for use with fluorescence 96-well plate readers. AccuClear™, AccuBlue™ NextGen and AccuBlue™ High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). AccuBlue™ Broad Range requires an instrument equipped to read blue fluorescence emission (Ex/Em 350/460 nm).

These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor™-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kit, which is designed for use on that instrument.

The AccuGreen™ High Sensitivity DNA Quantitation kit is designed for use on the Qubit® fluorometer. It can be used in the preprogrammed Qubit® dsDNA High Sensitivity assay, and is a direct replacement for the Qubit® dsDNA HS Assay kit.

Category: DNA Quantitation Kits

← FAQs

For detection of picogram levels of DNA on a microplate reader, we recommend our AccuBlue™ NextGen dsDNA Quantitation Kit (detection range 2.5 pg-3 ng dsDNA) which has higher accuracy and sensitivity than PicoGreen® in a 96-well assay format.

Also see our AccuClear™ Ultra High Sensitivity dsDNA Quantation Kit, which is a great all-purpose choice: it has high sensitivity with a broad linear detection range (30 pg-250 ng dsDNA).

If you own a Qubit® fluorometer, we recommend our AccuGreen™ High Sensitivity dsDNA Quantitation kit, which is designed for use with the Qubit® fluorometer. It is a direct replacement for the Qubit® dsDNA HS Assay kit.

Note that the lower limits of kit sensitivity may depend on the detection instrument used.

Category: DNA Quantitation Kits

← FAQs

← FAQs

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Shipping/Shelf-life

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.