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CD4 Monoclonal Mouse Antibody (SK3) – Biotium Choice

CD4 (clone SK3) is a validated mouse monoclonal antibody that recognizes CD4, a membrane glycoprotein of T lymphocytes. The antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance.

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Product Attributes

Antibody number

#P001

Antibody reactivity (target)

CD4

Biotium Choice Antibody

Primary

Antibody type

Biotium Choice Flow Antibodies, Primary

Host species

Mouse

Clonality

Monoclonal

Clone

SK3

Isotype

IgG1, kappa

Molecular weight

55 kDa

Synonyms

L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Human gene symbol

CD4

Entrez gene ID

920

SwissProt

P01730

Unigene

631659

Immunogen

Human peripheral blood T cells

Antibody target cellular localization

Plasma membrane

Verified antibody applications

Flow (surface) (verified)

Species reactivity

Human

Antibody application notes

Recommended concentration for flow cytometry: 5 uL per million cells/0.1 mL

Positive control

Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: PBS/0.1% BSA/0.05% azide

Antibody research areas

Immunology

Volume per assay

5 uL/test

Cell/tissue expression

Lymphocytes, Myeloid cells

Tumor expression

Leukemia/lymphoma

Conjugation
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Product Description

CD4 (clone SK3) is a mouse monoclonal antibody that recognizes the extracellular domain of CD4, a membrane glycoprotein of T lymphocytes. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. The antibody is available conjugated to CF® Dyes and Astral™ tandem dyes. They are supplied in PBS, 0.1% BSA, 0.05% azide.

CD4 interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. The majority of peripheral T-cell lymphomas are derived from the T-helper/regulatory cell subset so that most mature T-cell neoplasms are CD4/CD8. Anti-CD4 is used in the immunohistochemical staining of lymphoproliferative disorders to evaluate tumors with CD4 aberrant expression.

As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody. View our full catalog of Biotium Choice antibodies, optimized and validated for the best results in flow cytometry.

CD4 Monoclonal Mouse Antibody (SK3) - Biotium Choice

ConjugationEx/EmConc.SizeCatalog No.Dye Features
CF®405S411/431 nm100 ug/mL25 tests (125 uL)P001-405S-125CF®405S Features
100 tests (500 uL)P001-405S-500
CF®405M416/452 nm100 ug/mL25 tests (125 uL)P001-405M-125CF®405M Features
100 tests (500 uL)P001-405M-500
CF®405L413/547 nm200 ug/mL25 tests (125 uL)P001-405L-125CF®405L Features
100 tests (500 uL)P001-405L-500
CF®488A490/516 nm100 ug/mL25 tests (125 uL)P001-488A-125CF®488A Features
100 tests (500 uL)P001-488A-500
CF®503R503/532 nm50 ug/mL25 tests (125 uL)P001-503R-125CF®503R Features
100 tests (500 uL)P001-503R-500
CF®514516/549 nm100 ug/mL25 tests (125 uL)P001-514-125CF®514 Features
100 tests (500 uL)P001-514-500
CF® 568562/584 nm50 ug/mL25 tests (125 uL)P001-568-125CF®568 Features
100 tests (500 uL)P001-568-500
CF® 575575/601 nm25 ug/mL25 tests (125 uL)P001-575-125
100 tests (500 uL)P001-575-500
RPE-Astral™616496, 546, 566/617 nm100 ug/mL25 tests (125 uL)P001-R616-125
100 tests (500 uL)P001-R616-500
CF®647Plus652/668 nm25 ug/mL25 tests (125 uL)P001-647P-125
100 tests (500 uL)P001-647P-500
CF®660C667/685 nm50 ug/mL25 tests (125 uL)P001-660C-125CF®660C Features
100 tests (500 uL)P001-660C-500
CF®700696/721 nm25 ug/mL25 tests (125 uL)P001-700-125CF®700 Features
100 tests (500 uL)P001-700-500
APC-Astral™813633, 638/813 nm50 ug/mL25 tests (125 uL)P001-A813-125
100 tests (500 uL)P001-A813-500
R-PE496, 546, 565/576 nm25 ug/mL25 tests (125 uL)P001-RPE-125
100 tests (500 uL)P001-RPE-500