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CD4 Monoclonal Mouse Antibody (SK3) – Biotium Choice

CD4 (clone SK3) is a validated mouse monoclonal antibody that recognizes CD4, a membrane glycoprotein of T lymphocytes. The antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance.

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Product Attributes

Antibody number


Antibody reactivity (target)


Biotium Choice Antibody


Antibody type

Biotium Choice Flow Antibodies, Primary

Host species







IgG1, kappa

Molecular weight

55 kDa


L3T4; Leu3; Ly-4; Lymphocyte antigen CD4; p55; T cell antigen T4/LEU3; T cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

Human gene symbol


Entrez gene ID







Human peripheral blood T cells

Antibody target cellular localization

Plasma membrane

Verified antibody applications

Flow (surface) (verified)

Species reactivity


Antibody application notes

Recommended concentration for flow cytometry: 5 uL per million cells/0.1 mL

Positive control

Human peripheral blood mononuclear cells (PBMCs), CCRF-CEM or HL-60

Shipping condition

Room temperature

Storage Conditions

Store at 2 to 8 °C, Protect fluorescent conjugates from light

Shelf life

Guaranteed for at least 24 months from date of receipt when stored as recommended

Regulatory status

For research use only (RUO)

Antibody/conjugate formulation

Conjugates: PBS/0.1% BSA/0.05% azide

Antibody research areas


Volume per assay

5 uL/test

Cell/tissue expression

Lymphocytes, Myeloid cells

Tumor expression


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Catalog #
25 tests
100 tests
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Product Description

CD4 (clone SK3) is a mouse monoclonal antibody that recognizes the extracellular domain of CD4, a membrane glycoprotein of T lymphocytes. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. The antibody is available conjugated to CF® Dyes and Astral™ tandem dyes. They are supplied in PBS, 0.1% BSA, 0.05% azide.

CD4 interacts with major histocompatibility complex class II antigens and is also a receptor for the human immunodeficiency virus. This protein is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. The majority of peripheral T-cell lymphomas are derived from the T-helper/regulatory cell subset so that most mature T-cell neoplasms are CD4/CD8. Anti-CD4 is used in the immunohistochemical staining of lymphoproliferative disorders to evaluate tumors with CD4 aberrant expression.

As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody. View our full catalog of Biotium Choice antibodies, optimized and validated for the best results in flow cytometry.

CD4 Monoclonal Mouse Antibody (SK3) - Biotium Choice

ConjugationEx/EmConc.SizeCatalog No.Dye Features
CF®405S411/431 nm100 ug/mL25 tests (125 uL)P001-405S-125CF®405S Features
100 tests (500 uL)P001-405S-500
CF®405M416/452 nm100 ug/mL25 tests (125 uL)P001-405M-125CF®405M Features
100 tests (500 uL)P001-405M-500
CF®405L413/547 nm200 ug/mL25 tests (125 uL)P001-405L-125CF®405L Features
100 tests (500 uL)P001-405L-500
CF®488A490/516 nm100 ug/mL25 tests (125 uL)P001-488A-125CF®488A Features
100 tests (500 uL)P001-488A-500
CF®503R503/532 nm50 ug/mL25 tests (125 uL)P001-503R-125CF®503R Features
100 tests (500 uL)P001-503R-500
CF®514516/549 nm100 ug/mL25 tests (125 uL)P001-514-125CF®514 Features
100 tests (500 uL)P001-514-500
CF® 568562/584 nm50 ug/mL25 tests (125 uL)P001-568-125CF®568 Features
100 tests (500 uL)P001-568-500
CF® 575575/601 nm25 ug/mL25 tests (125 uL)P001-575-125
100 tests (500 uL)P001-575-500
RPE-Astral™616496, 546, 566/617 nm100 ug/mL25 tests (125 uL)P001-R616-125
100 tests (500 uL)P001-R616-500
CF®647Plus652/668 nm25 ug/mL25 tests (125 uL)P001-647P-125
100 tests (500 uL)P001-647P-500
CF®660C667/685 nm50 ug/mL25 tests (125 uL)P001-660C-125CF®660C Features
100 tests (500 uL)P001-660C-500
CF®700696/721 nm25 ug/mL25 tests (125 uL)P001-700-125CF®700 Features
100 tests (500 uL)P001-700-500
APC-Astral™813633, 638/813 nm50 ug/mL25 tests (125 uL)P001-A813-125
100 tests (500 uL)P001-A813-500
R-PE496, 546, 565/576 nm25 ug/mL25 tests (125 uL)P001-RPE-125
100 tests (500 uL)P001-RPE-500
APC651/660 nm50 ug/mL25 tests (125 uL)P001-APC-125
100 tests (500 uL)P001-APC-500