CD45 Mouse Monoclonal Antibody (2D1) – Biotium Choice
CD45 (clone 2D1) is a validated mouse monoclonal antibody that recognizes CD45, a transmembrane glycoprotein expressed in hematopoietic cells. The antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance.
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Product Description
CD45 (clone 2D1) is a mouse monoclonal antibody that recognizes transmembrane glycoprotein CD45. This antibody belongs to the Biotium Choice list of select antibodies that have been validated and optimized in-house for optimal performance. The antibody is available conjugated to CF® Dyes and Astral™ tandem dyes. They are supplied in PBS, 0.1% BSA, 0.05% azide.
CD45, also designated CD45R and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. This MAb reacts with all isoforms of CD45R expressed by all hematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes (Workshop IV). Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
As an isotype control, we recommend Isotype Control, Monoclonal Mouse IgG1 Kappa (IGG1/1331) for this antibody.
Discover Sharper Signals and Unmatched Panel Flexibility with Biotium Choice Antibodies
Powered by CF® Dyes and Astral Leap™ Tandem Dyes
Biotium Choice antibodies are carefully curated and validated in-house to offer exceptional signal-to-noise. Labeled with our advanced CF® Dyes and Astral Leap™ tandem dyes, they are our top-recommended antibodies for flow cytometry and other applications.
Biotium Choice Antibody Features
- Robust and validated clones against common targets
- Developed and optimized for flow cytometry and other applications
- Conjugated to bright, photostable CF® Dyes for superior signal and clarity
- Also available with Astral Leap™ tandem dyes for expanding multiplexing
- New antibody clones and dye conjugates continuously in development
View our full catalog of Biotium Choice antibodies
Identifying Biotium validated antibodies
Biotium scientists validate and optimize select antibodies in our catalog, marked with a check mark next to the product name. This symbol indicates the antibody is highly recommended for the specified application(s).
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CD45 Monoclonal Mouse Antibody (2D1) - Biotium Choice
Conjugation | Ex/Em | Conc. | Size | Catalog No. | Dye Features |
---|---|---|---|---|---|
CF®405M | 416/452 nm | 200 ug/mL | 25 tests (125 uL) | P012-405M-125 | CF®405M Features |
100 tests (500 uL) | P012-405M-500 | ||||
CF®488A | 490/516 nm | 200 ug/mL | 25 tests (125 uL) | P012-488A-125 | CF®488A Features |
100 tests (500 uL) | P012-488A-500 | ||||
RPE-Astral™616 | 496, 546, 566/617 nm | 100 ug/mL | 25 tests (125 uL) | P012-R616-125 | |
100 tests (500 uL) | P012-R616-500 | ||||
CF®647Plus | 652/668 nm | 100 ug/mL | 25 tests (125 uL) | P012-647P-125 | |
100 tests (500 uL) | P012-647P-500 | ||||
CF®820 | 822/835 nm | 100 ug/mL | 25 tests (125 uL) | P012-820-125 | CF®820 Features |
100 tests (500 uL) | P012-820-500 | ||||
APC-Astral™813 | 633, 638/813 nm | 100 ug/mL | 25 tests (125 uL) | P012-A813-125 | |
100 tests (500 uL) | P012-A813-500 | ||||
R-PE | 496, 546, 565/576 nm | 50 ug/mL | 25 tests (125 uL) | P012-RPE-125 | |
100 tests (500 uL) | P012-RPE-500 | ||||
APC | 651/660 nm | 100 ug/mL | 25 tests (125 uL) | P012-APC-125 | |
100 tests (500 uL) | P012-APC-500 |