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CellBrite NIR Cytoplasmic Membrane Dyes

Biotium’s CellBrite™ NIR Cytoplasmic Membrane Dyes are ready-to-use dye delivery solutions that can be added directly to normal culture media to uniformly label cells in suspension or adherent cultures.

CellBrite NIR Cytoplasmic Membrane Dyes
CellBrite NIR Cytoplasmic Membrane DyesCellBrite NIR Cytoplasmic Membrane DyesCellBrite NIR Cytoplasmic Membrane DyesCellBrite NIR Cytoplasmic Membrane Dyes
Catalog #

30070, 30077, 30078, - 30079View allHide

Size

100 uL

Dye color

CellBrite NIR680, CellBrite NIR790, CellBrite NIR770, CellBrite NIR750

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Product Description

Biotium’s CellBrite™ NIR Cytoplasmic Membrane Dyes are ready-to-use dye delivery solutions that can be added directly to normal culture media to uniformly label cells in suspension or adherent cultures.

Carbocyanine dyes label cytoplasmic membranes and intracellular membrane structures efficiently and permanently (1). They have been used as tracers in cell–cell fusion (2,3), cellular adhesion (4,5), and migration (6) applications due to their properties of low cytotoxicity and high resistance to intercellular transfer. However, the lipophilic nature of these dyes pose an obstacle to uniform cellular labeling. Although structurally related PKH dyes have been developed and optimized for cell labeling, the procedure requires multiple steps and subjects cells to an iso-osmotic mannitol loading medium (7,8).  A variety of carbocyanine dyes with different optical properties are available, allowing cell populations to be marked with distinct fluorescent colors for identification after mixing. Double labeling can identify cells that have fused or formed stable clusters.

CellBrite™ NIR Cytoplasmic Membrane Dyes are novel near-infrared carbocyanine dyes for labeling the cytoplasmic membranes of living cells. Due to their long emission wavelengths, near-infrared cell membrane stains can be used to label cells for near-infrared small animal imaging studies for non-invasive imaging of cell migration and cell homing (9). The dyes have long 18-carbon hydrophobic tails and an additional water-soluble group. These unique chemical structure elements make the dyes easy to dissolve while providing highly stable cytoplasmic membrane staining, unlike traditional carbocyanine dyes like DiI, DiO, and DiR, which are often difficult to dissolve or prone to precipitation during cell staining.

CellBrite™ NIR Cytoplasmic Membrane Dyes are available in the following colors: NIR680, NIR750, NIR770, and NIR790.

Biotium offers other CellBrite™ Cytoplasmic Membrane Stains in a variety of colors. For more information, download the product information sheet, or see Frequently Asked Questions about CellBrite™ Cytoplasmic Membrane Stains.

Product Information

Catalog No.Membrane DyeAbs/Em
30070CellBrite™ NIR680 683/724 nm
30077CellBrite™ NIR750 748/780 nm
30078CellBrite™ NIR770767/806 nm
30079CellBrite™ NIR790786/820 nm

References

Cat#30079
1. Science Advances (2017), doi: 10.1126/sciadv.1700521

 

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Support & Faq

CellBrite™ Cytoplasmic Membrane Stains

CellBrite™ and CellBrite™ Fix dyes are designed to label cell membranes. Original CellBrite™ dyes contain lipophilic carbocyanine dyes (DiB, Neuro-DiO, DiI, or DiD). These dyes accumulate in membrane lipids where they become fluorescent. Like other membrane dyes like VyBrant® DiO, VyBrant® DiI, ViBrant® DiR, CellMask™, CellVue® Claret, or PKH dyes, original CellBrite™ dyes can be fixed with formaldehyde, but are poorly retained after permeabilization with detergents or fixation with alcohol. CellBrite™ NIR dyes are CellBrite™ dyes with near-infrared fluorescence compatible with small animal NIR imaging systems.

CellBrite™ Fix dyes are a novel class of dye for labeling the cell surface. They are fluorogenic dyes that rapidly accumulate in the plasma membrane, where they react covalently with the cell surface. As a result, surface staining is well-retained after permeabilization or methanol fixation, with only a slight increase in intracellular fluorescence compared to formaldehyde fixation alone. CellBrite™ Fix dyes have better water solubility than classic lipophilic dyes, and as a result they yield much more uniform staining compared to lipophilic carbocyanine dyes like DiO and DiI. CellBrite™ Fix dyes are non-toxic and do not readily transfer between cells. They also can be used to stain yeast and bacteria (gram-positive or gram-negative).

Vybrant® and CellMask™ are trademarks of Thermo Fisher Scientific. CellVue® is a registered trademark of Millipore Sigma.

Category: CellBrite™ Cytoplasmic Membrane Stains

← FAQs

Original CellBrite™ Cytoplasmic Membrane Stains are lipophilic carbocyanine dyes. These dyes undergo an increase in fluorescence when they insert into lipid bilayers. Lipophilic carbocyanine dyes stably label the plasma membrane and other intracellular membranes of cells. They also can be used to stain fixed cells or artificial lipid bilayers.

CellBrite™ Fix Membrane Stains are fluorogenic membrane dyes that react covalently with proteins on the cell surface. They have better water solubility than original CellBrite™ dyes and as a result generally give more even staining of the cell surface compared to original CellBrite™ dyes. CellBrite™ Fix are well retained after fixation and permeabiliation or methanol fixation. However, CellBrite™ Fix dyes cannot be used to stain the plasma membrane of cells after they are already fixed (the dyes stain intracellular membranes of fixed cells).

For all membrane stains, if cells are returned to culture after staining, membrane internalization will occur over time, resulting in predominantly intracellular staining.

Category: CellBrite™ Cytoplasmic Membrane Stains

← FAQs

Lipophilic carbocyanine dyes like our original CellBrite™ Cytoplasmic Membrane Stains have been used to stain neuronal cells in culture for several weeks, and in vivo for up to a year. The dyes do not appreciably affect cell viability, and do not readily transfer between cells with intact membranes, allowing cell migration and tracking studies in mixed populations. Over time, the dyes will be internalized and become intracellular as membranes turn over. Long term stability of labeling may vary between cell types, depending on rates of membrane turnover or cell division.

CellBrite™ Fix Membrane Stains label the cell surface covalently. Like original CellBrite™ dyes, they are non-toxic and do not readily transfer between cells, but will be internalized and become intracellular as membranes turn over.

Category: CellBrite™ Cytoplasmic Membrane Stains

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Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.