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CellBrite NIR Cytoplasmic Membrane Dyes

Biotium’s CellBrite™ NIR Cytoplasmic Membrane Dyes are ready-to-use dye delivery solutions that can be added directly to normal culture media to uniformly label cells in suspension or adherent cultures.

CellBrite NIR Cytoplasmic Membrane Dyes
CellBrite NIR Cytoplasmic Membrane DyesCellBrite NIR Cytoplasmic Membrane DyesCellBrite NIR Cytoplasmic Membrane DyesCellBrite NIR Cytoplasmic Membrane Dyes
Catalog #

30070, 30077, 30078, - 30079View allHide

Size

100 uL

Dye color

CellBrite NIR680, CellBrite NIR790, CellBrite NIR770, CellBrite NIR750

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Product Description

Biotium’s CellBrite™ NIR Cytoplasmic Membrane Dyes are ready-to-use dye delivery solutions that can be added directly to normal culture media to uniformly label cells in suspension or adherent cultures.

Carbocyanine dyes label cytoplasmic membranes and intracellular membrane structures efficiently and permanently (1). They have been used as tracers in cell–cell fusion (2,3), cellular adhesion (4,5), and migration (6) applications due to their properties of low cytotoxicity and high resistance to intercellular transfer. However, the lipophilic nature of these dyes pose an obstacle to uniform cellular labeling. Although structurally related PKH dyes have been developed and optimized for cell labeling, the procedure requires multiple steps and subjects cells to an iso-osmotic mannitol loading medium (7,8).  A variety of carbocyanine dyes with different optical properties are available, allowing cell populations to be marked with distinct fluorescent colors for identification after mixing. Double labeling can identify cells that have fused or formed stable clusters.

CellBrite™ NIR Cytoplasmic Membrane Dyes are novel near-infrared carbocyanine dyes for labeling the cytoplasmic membranes of living cells. Due to their long emission wavelengths, near-infrared cell membrane stains can be used to label cells for near-infrared small animal imaging studies for non-invasive imaging of cell migration and cell homing (9). The dyes have long 18-carbon hydrophobic tails and an additional water-soluble group. These unique chemical structure elements make the dyes easy to dissolve while providing highly stable cytoplasmic membrane staining, unlike traditional carbocyanine dyes like DiI, DiO, and DiR, which are often difficult to dissolve or prone to precipitation during cell staining.

CellBrite™ NIR Cytoplasmic Membrane Dyes are available in the following colors: NIR680, NIR750, NIR770, and NIR790.

Biotium offers other CellBrite™ Cytoplasmic Membrane Stains in a variety of colors. For more information, download the product information sheet, or see Frequently Asked Questions about CellBrite™ Cytoplasmic Membrane Stains.

Product Information

Catalog No.Membrane DyeAbs/Em
30070CellBrite™ NIR680 683/724 nm
30077CellBrite™ NIR750 748/780 nm
30078CellBrite™ NIR770767/806 nm
30079CellBrite™ NIR790786/820 nm
 

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Support & Faq

CellBrite™ Cytoplasmic Membrane Stains

CellBrite cytoplasmic membrane stains are lipophilic carbocyanine dyes. These dyes undergo an increase in fluorescence when they insert into lipid bilayers. Lipophilic carbocyanine dyes stably label the plasma membrane and other intracellular membranes of cells. They also can be used to stain artificial lipid bilayers.

Category: CellBrite™ Cytoplasmic Membrane Stains

← FAQs

Lipophilic carbocyanine dyes have been used to stain neuronal cells in culture for several weeks, and in vivo for up to a year. The dyes do not appreciably affect cell viability, and do not readily transfer between cells with intact membranes, allowing cell migration and tracking studies in mixed populations. Stability of labeling may vary between cell types, depending on rates of membrane turnover or cell division.

Category: CellBrite™ Cytoplasmic Membrane Stains

← FAQs

Cells can be fixed with formaldehyde after labeling with CellBrite dyes. Lipophilic carbocyanine dyes like the CellBrite dyes have also been used to stain cells or tissues after formaldehyde fixation. Permeabilization of cells with detergents or solvents, or mounting medium containing glycerol may adversely affect staining. Permeabilization with digitonin (10 ug/mL to 1 mg/mL) has been reported to be compatible with lipophilic carbocyanine dye staining.

Category: CellBrite™ Cytoplasmic Membrane Stains

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Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions. Expedited shipment may be requested at time of checkout. Please note that products with recommended storage at 4°C or -20°C may ship at ambient temperature. This will not affect product performance. When you receive the product, place it under the recommended storage conditions.