Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits
Mix-n-Stain™ small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step.
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Product Description
Mix-n-Stain™ small ligand labeling kits are designed for rapid labeling of low molecular weight (MW ~150 -5000) and high affinity biological ligands or substrates without a purification step. The ligand to be labeled must contain an aliphatic amine group that is biologically inert. The amino group will form a covalent linkage with the provided reactive CF® dyes. For example, suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligand derivatives that have an aliphatic amine. For more information, please download the Mix-n-Stain™ Small Ligand Labeling Kit Flyer and the Product Information Sheet.
Mix-n-Stain™ small ligand labeling kits for cell surface labeling are available in your choice of 10 CF® dye colors for labeling ligands for cell surface targets, and five CF® dye colors for ligands that bind intracellular targets. We also offer TMP-PEG3-Amine for making fluorescent TMP ligands. Also see our Mix-n-Stain™ Antibody Labeling Kits and other Reactive Dyes and Biotin Reagents.
Dye | Catalog no. | Spectrally similar dyes | Ex (nm) | Em (nm) | Staining |
---|---|---|---|---|---|
CF®405M | 92362 | Pacific Blue™, BD Horizon™ V450 | 408 | 452 | Surface |
CF®488A | 92350 | FITC, Alexa Fluor® 488 | 490 | 515 | Surface |
CF®568 | 92351 | Rhodamine Red, Alexa Fluor® 568 | 562 | 583 | Surface |
CF®594 | 92352 | Texas Red®, Alexa Fluor® 594 | 593 | 614 | Surface |
CF®633 | 92353 | Cy®5, Alexa Fluor® 633 | 630 | 650 | Surface |
CF®640R | 92354 | Cy®5, Alexa Fluor® 647 | 642 | 662 | Surface |
CF®647 | 92359 | Cy®5, Alexa Fluor® 647 | 650 | 665 | Surface |
CF®660C | 92360 | Alexa Fluor® 660 | 667 | 685 | Surface |
CF®680 | 92361 | Cy®5.5, Alexa Fluor® 660, IRDye®680LT | 681 | 698 | Surface |
CF®680R | 92355 | Cy®5.5, Alexa Fluor® 660, IRDye®680LT | 680 | 701 | Surface |
CF®408 | 92356 | Pacific Blue™, BD Horizon™ V450 | 408 | 450 | Intracellular |
CF®500 | 92357 | FITC, Alexa Fluor® 488 | 500 | 510 | Intracellular |
CF®540 | 92358 | Tetramethylrhodamine (TAMRA), TRITC, Alexa Fluor® 546, Cy®3 | 540 | 570 | Intracellular |
CF®555 | 92364 | Alexa Fluor® 555, Cy®3, TRITC | 555 | 585 | Intracellular |
CF®650 | 92363 | Cy®5, Alexa Fluor® 647 | 650 | 670 | Intracellular |