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Mix-n-Stain™ CF® Dye Small Ligand Labeling Kits

Mix-n-Stain™ small ligand labeling kits are designed for rapid labeling of low molecular weight (MW approx. 150 -5000) and relatively high affinity biological ligands (or substrates) without a purification step.

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Product Description

Mix-n-Stain™ small ligand labeling kits are designed for rapid labeling of low molecular weight (MW ~150 -5000) and high affinity biological ligands or substrates without a purification step.  The ligand to be labeled must contain an aliphatic amine group that is biologically inert.  The amino group will form a covalent linkage with the provided reactive CF® dyes.  For example, suitable ligands or substrates include SNAP-tag®, CLIP-tag™ and HaloTag® ligand derivatives that have an aliphatic amine. For more information, please download the Mix-n-Stain™ Small Ligand Labeling Kit Flyer and the Product Information Sheet.

Mix-n-Stain™ small ligand labeling kits for cell surface labeling are available in your choice of 10 CF® dye colors for labeling ligands for cell surface targets, and five CF® dye colors for ligands that bind intracellular targets. We also offer TMP-PEG3-Amine for making fluorescent TMP ligands. Also see our Mix-n-Stain™ Antibody Labeling Kits and other Reactive Dyes and Biotin Reagents.

DyeCatalog no.Spectrally similar dyesEx (nm)Em (nm)Staining
CF®405M92362Pacific Blue™, BD Horizon™ V450408452Surface
CF®488A92350FITC, Alexa Fluor® 488490515Surface
CF®56892351Rhodamine Red, Alexa Fluor® 568562583Surface
CF®59492352Texas Red®, Alexa Fluor® 594593614Surface
CF®63392353Cy®5, Alexa Fluor® 633630650Surface
CF®640R92354Cy®5, Alexa Fluor® 647642662Surface
CF®64792359Cy®5, Alexa Fluor® 647650665Surface
CF®660C92360Alexa Fluor® 660667685Surface
CF®68092361Cy®5.5, Alexa Fluor® 660, IRDye®680LT681698Surface
CF®680R92355Cy®5.5, Alexa Fluor® 660, IRDye®680LT680701Surface
CF®40892356Pacific Blue™, BD Horizon™ V450408450Intracellular
CF®50092357FITC, Alexa Fluor® 488500510Intracellular
CF®54092358Tetramethylrhodamine (TAMRA), TRITC, Alexa Fluor® 546, Cy®3540570Intracellular
CF®55592364Alexa Fluor® 555, Cy®3, TRITC555585Intracellular
CF®65092363Cy®5, Alexa Fluor® 647650670Intracellular
SNAP-tag and CLIP-tag are trademarks or registered trademarks of New England BioLabs; HaloTag is a registered trademark of Promega Corporation; Pacific Blue, Texas Red, and Alexa Fluor are trademarks or registered trademarks of Thermo Fisher Scientific; BD Horizon is a trademark of BD Biosciences; Cy Dye is a registered trademark of GE Healthcare; IRDye is a registered trademark of LI-COR Biosciences.

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