NucSpot® Live Cell Nuclear Stains are cell-membrane permeable DNA dyes that specifically stain nuclei in live or fixed cells. They have excellent specificity for DNA without the need for a wash step, and they have low toxicity for live cell imaging. The dyes are supplied as 1000X stock solutions in DMSO, and are supplied with a vial of verapamil, which may increase probe retention and staining in some cell types.
NucSpot® Live 488 stains nuclei with green fluorescence (Ex/Em 500/515 nm). NucSpot® Live 650 stains nuclei with far-red fluorescence (Ex/Em 650/675 nm) for detection in the Cy®5 channel. NucSpot® Live 650 dye also is compatible with super-resolution imaging by SIM or STED, and has been validated in STORM (Ref. 3). Note: NucSpot® Live 488 and NucSpot® Live 650 have dim blue fluorescence in the DAPI channel, and may not be suitable for multicolor imaging with blue probes.
NucSpot® Live Cell Nuclear Stains can be used to stain live gram-positive bacteria, but do not stain live gram-negative bacteria or yeast. See our Cellular Stains Table for more information on how our dyes stain various organisms.
Also see our wide selection of other Membrane and Organelle Stains, or download the Cellular Stains Brochure.
Cy5 is a registered trademark of GE Healthcare.