Buffers, Accessories & Background Quenchers

A blocking agent commonly used to minimize background noise in immunodetection procedures such as western blotting and immunostaining.

Gelatin from fish skin that can be added to blocking buffers to minimize non-specific antibody binding in immuno-detection procedures such as western blotting and cell staining.

Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.

Small polyethylene cell scrapers for harvesting cells or cell lysates from 96-, 48- and 24-well plates.

An alternative to plastic microcentrifuge tubes for up to 200 uL sample volumes.

These sterile 0.22 um filters are useful for filtering 100 uL to 1 mL of aqueous solutions with only 10 uL hold-up volume.

A concentrated 5X buffer solution for one step permeabilization and blocking for immunofluorescence staining for fluorescence microscopy.

Ready-to-use permeabilization buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.

Pens to draw a water repellent circle around slide mounted tissue to minimize the use of valuable reagents by keeping the liquid pooled in a single droplet. Available in a regular (4 mm tip) and mini (2.5 mm tip) sizes.

A polysorbate surfactant commonly used as a blocking and washing agent in Western blotting and other immunostaining assays.

Ultrafiltration devices for simple spin-column purification of proteins or other molecules by size separation with two molecular weight cut-off options: MWCO=10 kD (10K) and MWCO=3 kD (3K).

Suitable for use in molecular biology applications that require high quality, ultra-pure water.

A convenient 4X sample loading buffer for protein gel electrophoresis. The loading buffer is optimized for use with denaturing SDS-PAGE.

AntiFix™ Universal Antigen Retrieval Buffer provides excellent heat unmasking for a wide variety of targets at neutral pH, reducing the need for buffer optimization.

Unique hardset antifade that quenches lipofuscin autofluorescence. Choose with mounting medium with DAPI, far-red NucSpot® 640, or without nuclear stain.

Biotium’s hardset antifade mounting medium with NucSpot® 640 far-red nuclear counterstain.