Chloride, pH, Zinc & Other Indicators

5(6)-CFDA (full name: 5-(and-6)-Carboxyfluorescein diacetate) is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, CFDA is hydrolyzed by intracellular esterases to carboxyfluorescein (see #51013 ).

Membrane-permeant AM ester form of Mag-Fluo-4, a fluorescent magnesium and calcium ion indicator that is an analog of Fluo-4.

Membrane-impermeant form of Mag-Fluo-4, a fluorescent magnesium and calcium ion indicator that is an analog of Fluo-4.

Nonionic detergent useful for solubilizing hydrophobic compounds to facilitate cell loading.

Zinquin is an UV-excitable, blue fluorescent zinc indicator. Zinc is believed to be involved in the suppression of apoptosis and thought to play important roles in many neural activities.

Zinquin is an UV-excitable, blue fluorescent zinc indicator. Zinquin ethyl ester is membrane-permeable and is hydrolyzed into Zinquin free acid once entering cells.

5-(and-6)-Carboxy-2′,7′-dichlorofluorescein diacetate, succinimidyl ester is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and nonfluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

5(6)-CFDA, SE (full name: 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester, also known as CFSE) is a useful fluorescent tracer that can passively diffuse into cells and covalently label intracellular proteins, resulting in long-term cell labeling. The reagent itself is colorless and non-fluorescent but becomes brightly green fluorescent once it is hydrolyzed by intracellular esterases.

Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic compounds like AM esters of calcium indicator dyes to facilitate cell loading.

Ready-to-use solution of Pluronic® F-127, a nonionic detergent useful for solubilizing hydrophobic dye molecules such as AM esters of calcium indicator dyes to facilitate cell loading.

MQAE (N-(Ethoxycarbonylmethyl)-6-methoxyquinolinium bromide) is an improved chloride indicator that has greater sensitivity to chloride than SPQ, and higher fluorescence quantum yield.

TPEN (Tetrakis-(2-pyridylmethyl)ethylenediamine) is an excellent heavy metal chelator while it does not affect calcium, magnesium, sodium, or potassium concentrations. Thus, TPEN is a useful tool to identify effects of heavy metals (zinc, iron, copper, manganese, etc.) on fluorescent indicators for other ions.

Flubi-2 is the hydrolyzed product of the site-directed fluorogenic probe Flubida-2 (#51022) and can be used as a standard for calibration.

The fluorescence of SPQ is specifically quenched by chloride via collision. Therefore, chloride concentration is measured by monitoring the degree of fluorescence decrease.

The pKa of 5-carboxy-2′,7′-dichlorosulfonefluorescein is near 4 and thus the dye is potentially an excellent pH indicator for acidic organelles.

6-CFDA is membrane-permeant and thus can be loaded into cells via incubation. Once inside cells, 6-CFDA is hydrolyzed by intracellular esterases to 6-carboxyfluorescein.