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Flow Cytometry

See below for an array of probes, kits, and accessory products for use in your flow cytometry experiments. We offer a wide selection of dead cell selective stains, as well as probes for monitoring apoptosis, proliferation, cell viability, cell cycle, and membrane potential. Biotium also carries labeled primary antibodies, labeled secondary antibodies, antibody labeling kits, buffers, and other accessory products.

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Mix-n-Stain™ Nanobody Thiol Labeling Kits

From: $195 Sizes: 5-20 ug labeling, 20-50 ug labelingCatalog #:
92585, 92586, 92587, 92588, 92589, 92590, 92591, 92592, 92593, 92594, 92595, 92596, 92597, - 92598View allHide

Label 5-50 ug of VHH (also known as Nanobodies®) containing a single free cysteine with Biotium’s bright and photostable CF® Dyes, with no purification after labeling.

ExoBrite™ EV Stain Enhancer (100X)

From: $50 Sizes: 5 x 0.5 mL, 0.5 mLCatalog #:
28002-T, - 28002View allHide

A unique additive that can be added to extracellular vesicle (EV) stain reactions to improve the staining specificity for applications like flow cytometry.

Propidium Iodide (PI)

From: $63 Sizes: 10 mL (1 mg/mL in Water), 2 mL (50 ug/mL in Buffer), 100 mgCatalog #:
40016, 40017, - 40048View allHide

A membrane-impermeant nucleic acid intercalator. The dye is commonly used to selectively stain dead cells in a mixed live-dead population and also used as a counterstain in multicolor fluorescent assays.

Annexin V Conjugates

From: $117 Sizes: 100 uL, 500 uLCatalog #:
29126, 29085, 29001, 29003, 29004, 29005, 29008, 29009, 29010, 29011, 29012, 29013, 29014, 29069, 29045-100uL, 29045-500uL, 29057-100uL, 29083, - 29057-500uLView allHide

Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.

ExoBrite™ True EV Membrane Stains

From: $194 Sizes: 100 labelings, 500 labelingsCatalog #:
30137, 30137-T, 30136, 30136-T, 30129-T, 30130-T, 30129, - 30130View allHide

Lipophilic membrane dyes designed specifically for EV research and the best choice for general pan-EV staining. The dyes are validated to offer higher (near-complete) coverage of EVs with lower aggregation than other membrane dyes like PKH, DiO, and DiI.

NucSpot® 470 Nuclear Stain

From: $65 Sizes: 20 uL, 200 uLCatalog #:
40083-T, - 40083View allHide

Cell membrane-impermeant, nuclear-specific green counterstain. Suitable for fixed cells or staining dead cells in live cultures.

ExoBrite™ EV Surface Stain Sampler Kit, Green

From: $363 Sizes: 1 kitCatalog #: 30127

Kit includes each of ExoBrite™ 490/515 EV Surface Stains (CTB, WGA, and Annexin V) for assessing which stain offers the best coverage for EV samples of interest.

ViaFluor® SE Cell Proliferation Kits

From: $63 Sizes: Trial size (1 vial), Set of 10 vialsCatalog #:
30139, 30139-T, 30068-T, 30068, 30086-T, 30086, - 30050View allHide

Fixable cytoplasmic stains for monitoring cell division by flow cytometry. The dyes can also be used to track cell populations in co-culture.

ExoBrite™ WGA EV Staining Kits

From: $149 Sizes: 100 labelings, 500 labelingsCatalog #:
30123-T, 30123, 30124-T, 30124, 30125-T, 30125, 30126-T, - 30126View allHide

Fluorescent WGA conjugates that are optimized for bright staining of extracellular vesicles for flow cytometry.

Mix-n-Stain™ STORM CF® Dye Antibody Labeling Kits

From: $287 Sizes: 50 ug labelingCatalog #:
92549, 92552, 92557, 92556, 92555, 92554, 92553, 92551, - 92550View allHide

Label your antibody with one of our CF® Dyes specialized for STORM super-resolution microscopy. Labeling takes just 30 minutes without a purification step, and yields an antibody conjugate with a low degree of labeling optimal for STORM.

Recombinant Annexin V (Lyophilized)

From: $172 Sizes: 100 ug, 1 mg, 5 mgCatalog #:
29089-100ug, 29088-100ug, 29088, 29089, - 29089-5mgView allHide

Recombinant human Annexin V expressed from E. coli as a single, non-glycosylated polypeptide chain. The protein is available with no tag or a C-terminal 6X His tag.

ExoBrite™ Annexin EV Staining Kits

From: $154 Sizes: 100 labelings, 500 labelingsCatalog #:
30119-T, 30119, 30120-T, 30120, 30121-T, 30121, 30122-T, - 30122View allHide

Fluorescent Annexin V conjugates that are optimized for bright and low background staining of extracellular vesicles for flow cytometry.

Mix-n-Stain™ CF® Dye IgM Antibody Labeling Kits

From: $162 Sizes: 25 ug labeling, 100 ug labelingCatalog #:
92561, 92560, 92559, 92558, 92563, 92562, 92565, 92564, 92567, 92566, 92569, 92568, 92571, 92570, 92573, 92572, 92575, - 92574View allHide

Label your IgM antibody with one of our superior CF® Dyes in 15-30 minutes without a purification step.

ExoBrite™ CTB EV Staining Kits

From: $201 Sizes: 100 labelings, 500 labelingsCatalog #:
30111-T, 30111, 30112-T, 30112, 30113-T, 30113, 30114-T, - 30114View allHide

Fluorescent cholera toxin subunit B (CTB) conjugates that are optimized for bright and clean staining of extracellular vesicles for flow cytometry.

Oxazole Red (YO-PRO®-3), 1 mM in DMSO

From: $344 Sizes: 1 mLCatalog #: 40105

Oxazole Red, also known as YO-PRO®-3, is a far-red fluorescent, cell-impermeant nucleic acid stain that can be used to stain dead or fixed cells.

Oxazole Red Homodimer (YOYO®-3), 1 mM in DMSO

From: $515 Sizes: 200 uLCatalog #: 40106

Oxazole Red Homodimer, also known as YOYO®-3, is a far-red fluorescent, cell-impermeant nucleic acid stain that can be used to stain dead or fixed cells.