Viability PCR Reagents
Viability PCR is a powerful technology for the sensitive and rapid detection of viable microorganisms. Unlike time-consuming culturing methods, qPCR is a fast and sensitive method of detection. However, normal PCR does not distinguish between live and dead cells. With v-PCR using PMAxx™ or PMA, you get the speed, sensitivity and specificity of PCR, plus quantified viability. And because no culturing is required, you can even detect viable but not culturable (VBNC) bacteria. The v-PCR technology can be applied not only to bacteria but to other organisms like yeast, viruses and parasites.
As the inventor of both PMAxx™ and PMA v-PCR dyes, Biotium offers expertise, and the widest selection of v-PCR dyes, kits and accessory products.
To learn more about viability PCR, visit our technology page.
PMAxx™ Dye, 20 mM in H2O
40069-1ML, - 40069View allHide
Viability PCR dye, a new and improved version of the popular viability dye propidium monoazide (PMA).
PMA (Propidium Monoazide)
40013, - 40019View allHide
A photo-reactive DNA-binding dye typically used in viability PCR (v-PCR) of microorganisms like bacteria, viruses, and fungi.
Viability PCR Starter Kits
31075, 31075-X, 31076, - 31076-XView allHide
Kits designed to make it simple to start performing viability PCR with your choice of viability dye (PMA or PMAxx™).
PMA Enhancer for Gram Negative Bacteria, 5X Solution
This product improves PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria when performing viability PCR.
PMA-Lite™ 2.0 LED Photolysis Device
An optimized photoactivation device designed for photolysis of PMAxx™- and PMA- treated samples in viability PCR.
Glo-Plate™ 2.0 Blue LED Illuminator
A multi-functional blue LED light box that can be used for photolysis of PMAxx™ or PMA in viability PCR; or to develop the visible DNA gel stain DNAzure®.
PMA Real-Time PCR Bacterial Viability Kit – E. coli (uidA)
31050, - 31050-XView allHide
A kit designed to perform viability PCR on E. coli. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the uidA gene.
PMA Real-Time PCR Bacterial Viability Kit – E. coli O157:H7 (Z3276)
31037, - 31037-XView allHide
A kit designed to perform viability PCR on E. coli O157:H7. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the Z3276 gene.
PMA Real-Time PCR Bacterial Viability Kit – Legionella pneumophila (mip)
A kit designed to perform viability PCR on Legionella pneumophila. It contains the viability PCR dye PMA and primers to amplify the mip gene.
PMA Real-Time PCR Bacterial Viability Kit – Listeria monocytogenes (hly)
31051, - 31051-XView allHide
A kit designed to perform viability PCR on Listeria monocytogenes. It contains a viability dye (PMAxx™ or PMA) and primers to amplify the hly gene.
PMA Real-Time PCR Bacterial Viability Kit – Mycobacterium tuberculosis (groEL2)
A kit designed to perform viability PCR on Mycobacterium tuberculosis. It contains the viability PCR dye PMA and primers to amplify the groEL2 gene.
PMA Real-Time PCR Bacterial Viability Kit – Salmonella enterica (invA)
31033, - 31033-XView allHide
A kit designed to perform viability PCR on Salmonella enterica. It contains a viability PCR dye (PMAxx™ or PMA) and primers to amplify the invA gene.
PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (nuc)
A kit designed to perform viability PCR on Staphylococcus aureus. It contains the viability PCR dye PMA and primers to amplify the nuc gene.
PMA Real-Time PCR Bacterial Viability Kit – Staphylococcus aureus (mecA)
A kit designed to perform viability PCR on MRSA. It contains the viability PCR dye PMA and primers to amplify the mecA gene.
Ethidium Monoazide Bromide (EMA)
A photo-activated fluorescent nucleic acid binding dye used to selectively and covalently label dead cells in the presence of live cells for viability PCR, fluorescent microscopy, and flow cytometry.