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Fluorescent CF® Dyes CF®583 & CF®583R Dyes

CF®583 & CF®583R Dyes

  • CF®583 produces brighter conjugates than Cy®3.5
  • CF®583R is a superior green-excitable dye for STORM
  • Excellent water solubility
CF® Dye Spectrum:
CF®350 Dye
CF®350 is a blue fluorescent dye
CF®405S Dye
Improved brightness & photostability for the 405 nm laser line
CF®405M Dye
Improved brightness & photostability for the 405 nm laser line
CF®430 & CF®440 Dyes
Photostable 405 nm-excitable dyes with green fluorescence
CF®405L Dye
A 405 nm-excitable dye with green fluorescence emission
CF®450 Dye
A spectrally unique, 405 nm-excitable green dye
CF®488A Dye
A superior green fluorescent dye
CF®503R Dye
A spectrally unique green dye ideal for spectral flow cytometry
CF®514 Dye
Alternative green fluorescent dye
CF®532 Dye
A bright green fluorescent dye for the 532 nm laser
CF®543 Dye
An orange fluorescent dye ideal for the 543 nm laser
CF®535ST Dye
A red fluorescent dye designed for STORM super-resolution imaging
CF®550R Dye
A spectrally unique orange/red dye ideal for spectral flow cytometry
CF®555 Dye
A bright and photostable orange-red dye
CF®568 Dye
Outshines Alexa Fluor®568
CF®570
Bright red fluorescent dye
CF®583 & CF®583R Dyes
Bright red fluorescent dyes
CF®594 Dye
Truly the brightest deep red dye
CF®597R Dye
Unique green-excited dye for STORM
RPE-Astral™616
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®620R Dye
A bright and photostable far-red dye
CF®633 Dye
The best dye for 633/635 laser lines
CF®640R Dye
A highly photostable far-red dye
CF®647 Dye
A highly fluorescent far-red dye
CF®660C & CF®660R
Superior alternatives to Alexa Fluor® 660
CF®680 & CF®680R
Two outstanding 680 nm-excitable dyes
CF®700 Dye
Brighter alternative to Alexa Fluor® 700
CF®710 Dye
Near-infrared CF® Dye with exceptional photostability
CF®725 Dye
Near-infrared CF® Dye with exceptional photostability
CF®740 Dye
Near-infrared CF® Dye with exceptional photostability
CF®750 Dye
Near-infrared CF® Dye
CF®770 Dye
Near-infrared CF® Dye
CF®790 Dye
Near-infrared CF® Dye
APC-Astral™813
Novel long Stokes shift tandem dye for flow cytometry multiplexing
CF®800 Dye
Near-infrared CF® Dye
CF®820 Dye
Near-infrared CF® Dye
CF®850 Dye
Near-infrared CF® Dye
CF®870 Dye
Near-infrared CF® Dye

CF®583 and CF®583R are bright red fluorescent dyes with emission around 609 nm and excitation by 561–568 nm lasers. CF®583 is a Cy®3.5 alternative, while CF®583R, a rhodamine-based dye, is a Texas Red® alternative and a strong FRET acceptor with R-PE. Both dyes yield brighter antibody conjugates than similar dyes (Figures 3, 4) and offer excellent water solubility for better bioconjugation and preserved specificity. Available in amine-reactive forms and various conjugates.

See products available with CF®583 and CF®583R. Additional conjugates or reactive dyes may be added upon request; contact techsupport@biotium.com to inquire.

For bright and photostable red dyes with a wide selection of reactive dyes, labeling kits, and conjugates, see our CF®568, and CF®594 dyes.

CF®583 Technical Summary

  • Abs/Em maxima: 583/606 nm
  • Extinction coefficient: 150,000
  • Molecular weight: ~3127
  • Excitation laser line: 561 to 568 nm
  • Alternative for: Cy®3.5

CF®583R Technical Summary

  • Abs/Em maxima: 586/609 nm
  • Extinction coefficient: 100,000
  • Molecular weight: ~773
  • Excitation laser line: 561 to 568 nm
  • Alternative for: Cy®3.5, Texas Red®
Figure 1. Normalized absorption and emission spectra of CF®583 goat anti-mouse conjugate in PBS.
Figure 2. Normalized absorption and emission spectra of CF®583R goat anti-mouse conjugate in PBS.
Figure 3. CF®583 produces brighter conjugates at a lower degree of labeling than Cy®3.5. Relative fluorescence of goat anti-mouse conjugates of the indicated dy... See More
Figure 4. CF®583R produces brighter conjugates at a lower degree of labeling than Cy®3.5 and TexasRed®. Relative fluorescence of goat anti-mouse conjugates of t... See More

Superior Brightness over Cy®3.5

Thanks to its enhanced solubility, CF®583R delivers significantly lower non-specific background than Cy®3.5. In methanol-fixed HeLa cells, staining with CF®583R-conjugated secondary antibody without primary antibody showed minimal background—unlike Cy®3.5, which exhibited high non-specific signal. Choose CF®583R for brighter and more reliable fluorescence.

Figure 5. Due to its enhanced solubility, CF®583R has low non-specific background compared to Cy®3.5. Methanol-fixed HeLa cells were stained with mouse anti-tub... See More

Superior Performance for STORM Imaging

CF®583R and CF®597R also deliver outstanding dSTORM performance with superior uniformity versus other green-excited dyes. Available as single-labeled secondary antibodies and Mix-n-Stain™ STORM Kits ideal for super-resolution imaging. Explore our unique tools for super-resolution microscopy.

Figure 6. Comparison of typical (d)STORM images obtained in fixed COS-7 cells for microtubules immunolabeled by different dyes. (d)STORM was performed under sta... See More

Helpful Tools & Resources

Visualize and export the fluorescent dye spectrum of any CF® Dye or other dyes from Biotium.
Learn the history of fluorophores in life science research as well as the development and advantages of CF® Dyes.
Check out our handy fluorophore selection tool to help choose the best CF® Dye for your next experiment.
Download our single page reference guide for all CF® Dye fluorophores. Includes recommended channels, applications, relative brightness, and photostability.
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CF®594 Dye

CF is a registered trademark of Biotium, Inc. Alexa Fluor is a registered trademark of Thermo Fisher Scientific; Cy Dye is a registered trademark of Cytiva.

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