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What instruments can I use with your DNA/RNA quantitation kits? Do they work on Qubit®?

The AccuGreen™ DNA Quantitation Kits are designed for use on the Qubit® fluorometer. The AccuGreen™ High Sensitivity DNA Quantitation Kit is a direct replacement for the Qubit® dsDNA HS Assay Kit from Thermo Fisher Scientific. The AccuGreen™ Broad Range DNA Quantitation Kit is a replacement for the Qubit® dsDNA BR Assay Kit from Thermo Fisher Scientific.

The AccuBlue® Broad Range RNA Quantitation is optimized for use with the Qubit® fluorometer using the pre-programmed RNA Broad Range program. It also can be used with fluorescence microplate readers equipped for detecting far-red fluorescence (Ex/Em 630/660 nm). The kit has a wide linear range of 5-1000 ng RNA, spanning those of both the Quant-iT™ RNA and Quant-iT™ RNA Broad Range Kits from Thermo Fisher Scientific.

The AccuClear® and AccuBlue® DNA Quantitation Kits are designed for use with fluorescence 96-well plate readers. AccuClear®, AccuBlue® NextGen and AccuBlue® High Sensitivity kits require an instrument equipped to read green fluorescence emission (similar to FITC). These assays also can be used with fluorometers such as the Qubit® (Thermo Scientific) and QuantiFluor®-P (Promega). However, due to different linear ranges of the assays, not all of these assays are compatible with the pre-programmed DNA quantitation programs on these instruments. For users who own a Qubit® fluorometer, we recommend using our AccuGreen™ kits, which are designed for use on that instrument.

The AccuBlue® Broad Range Kit requires an instrument equipped to read blue fluorescence (Ex/Em 350/460 nm).

<div> Quant-iT and Qubit are trademarks or registered trademarks of Thermo Fisher Scientific. QuantiFluor is a registered trademark of Promega.</div>

 

Category: DNA Quantitation Kits

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